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SASDSN9 – Buffer over-subtracted hen egg white lysozyme in 1 mol% ethylammonium nitrate

Lysozyme C experimental SAS data
Lysozyme C Kratky plot
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 1 mol% ethylammonium nitrate, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 27
Scattering approaches to unravel protein solution behaviors in ionic liquids and deep eutectic solvents: From basic principles to recent developments Advances in Colloid and Interface Science :103242 (2024)
Han Q, Veríssimo N, Bryant S, Martin A, Huang Y, Pereira J, Santos-Ebinuma V, Zhai J, Bryant G, Drummond C, Greaves T
RgGuinier 1.6 nm
Dmax 6.2 nm
VolumePorod 28 nm3

SASDSP9 – Buffer under-substracted hen egg white lysozyme in 1mol% ethylammonium nitrate

Lysozyme C experimental SAS data
Lysozyme C Kratky plot
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 1 mol% ethylammonium nitrate, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 27
Scattering approaches to unravel protein solution behaviors in ionic liquids and deep eutectic solvents: From basic principles to recent developments Advances in Colloid and Interface Science :103242 (2024)
Han Q, Veríssimo N, Bryant S, Martin A, Huang Y, Pereira J, Santos-Ebinuma V, Zhai J, Bryant G, Drummond C, Greaves T
RgGuinier 1.6 nm
Dmax 6.2 nm
VolumePorod 23 nm3

SASDSW8 – Apo form of vaccinia virus polymerase holoenzyme E9-A20-D4

DNA polymeraseDNA polymerase processivity factor component OPG148Uracil-DNA glycosylase experimental SAS data
DNA polymerase DNA polymerase processivity factor component OPG148 Uracil-DNA glycosylase Kratky plot
Sample: DNA polymerase monomer, 117 kDa Vaccinia virus (strain … protein
DNA polymerase processivity factor component OPG148 monomer, 49 kDa Vaccinia virus (strain … protein
Uracil-DNA glycosylase monomer, 27 kDa Vaccinia virus (strain … protein
Buffer: 200 mM NaCl, 20 mM Tris-HCl and 1 mM TCEP, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 25
Structure and flexibility of the DNA polymerase holoenzyme of vaccinia virus. PLoS Pathog 20(5):e1011652 (2024)
Burmeister WP, Boutin L, Balestra AC, Gröger H, Ballandras-Colas A, Hutin S, Kraft C, Grimm C, Böttcher B, Fischer U, Tarbouriech N, Iseni F
RgGuinier 5.0 nm
Dmax 18.0 nm
VolumePorod 350 nm3

SASDU93 – multimodular GH16_3 containing a CBM and a catalytic domain

Glycosyl hydrolase, family 16 experimental SAS data
DADIMODO model
Sample: Glycosyl hydrolase, family 16 monomer, 58 kDa Christiangramia forsetii (strain … protein
Buffer: 10 mM MOPS, 100 mM NaCl, pH: 7.8
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 1
Unveiling the role of novel carbohydrate-binding modules in laminarin interaction of multimodular proteins from marine Bacteroidota during phytoplankton blooms. Environ Microbiol 26(5):e16624 (2024)
Zühlke MK, Ficko-Blean E, Bartosik D, Terrapon N, Jeudy A, Jam M, Wang F, Welsch N, Dürwald A, Martin LT, Larocque R, Jouanneau D, Eisenack T, Thomas F, Trautwein-Schult A, Teeling H, Becher D, Schweder T, Czjzek M
RgGuinier 3.8 nm
Dmax 12.9 nm
VolumePorod 76 nm3

SASDUA3 – Surface glycan binding protein (Cf-SGBP) from Christiangramia forsetii

PKD domain-containing protein experimental SAS data
DADIMODO model
Sample: PKD domain-containing protein monomer, 96 kDa Christiangramia forsetii (strain … protein
Buffer: 10 mM MOPS, 100 mM NaCl, pH: 7.8
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 1
Unveiling the role of novel carbohydrate-binding modules in laminarin interaction of multimodular proteins from marine Bacteroidota during phytoplankton blooms. Environ Microbiol 26(5):e16624 (2024)
Zühlke MK, Ficko-Blean E, Bartosik D, Terrapon N, Jeudy A, Jam M, Wang F, Welsch N, Dürwald A, Martin LT, Larocque R, Jouanneau D, Eisenack T, Thomas F, Trautwein-Schult A, Teeling H, Becher D, Schweder T, Czjzek M
RgGuinier 5.0 nm
Dmax 17.8 nm
VolumePorod 138 nm3

SASDUX7 – Aspergillus niger beta-glucosidase (bgl1), partially deglycosylated with Endoglycosidase H

beta-glucosidase experimental SAS data
GASBOR model
Sample: Beta-glucosidase dimer, 187 kDa Aspergillus niger protein
Buffer: 50 mM sodium acetate, pH: 5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Feb 15
Aspergillus niger beta-glucosidase (bgl1)
Estella Yee
RgGuinier 4.2 nm
Dmax 14.0 nm
VolumePorod 253 nm3

SASDV52 – Aspergillus niger beta-glucosidase (bgl1), native, glycosylated

beta-glucosidase experimental SAS data
DAMMIN model
Sample: Beta-glucosidase dimer, 187 kDa Aspergillus niger protein
Buffer: 50 mM sodium acetate, pH: 5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jun 4
Aspergillus niger beta-glucosidase (bgl1)
Estella Yee
RgGuinier 4.4 nm
Dmax 13.9 nm
VolumePorod 277 nm3

SASDT75 – Trypanosoma brucei gambiense invariant surface glycoprotein 43 (ISG43)

T. brucei spp.-specific protein experimental SAS data
BILBOMD model
Sample: T. brucei spp.-specific protein monomer, 38 kDa Trypanosoma brucei gambiense … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
Sülzen H, Volkov AN, Geens R, Zahedifard F, Stijlemans B, Zoltner M, Magez S, Sterckx YG, Zoll S
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 53 nm3

SASDT85 – Trypanosoma brucei gambiense invariant surface glycoprotein 64 (ISG64)

64 kDa invariant surface glycoprotein, putative experimental SAS data
BILBOMD model
Sample: 64 kDa invariant surface glycoprotein, putative monomer, 40 kDa Trypanosoma brucei gambiense … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
Sülzen H, Volkov AN, Geens R, Zahedifard F, Stijlemans B, Zoltner M, Magez S, Sterckx YG, Zoll S
RgGuinier 3.1 nm
Dmax 11.2 nm
VolumePorod 56 nm3

SASDT95 – Trypanosoma brucei gambiense invariant surface glycoprotein 75 (ISG75)

ISG75 experimental SAS data
BILBOMD model
Sample: ISG75 monomer, 50 kDa Trypanosoma brucei gambiense protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 25
Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
Sülzen H, Volkov AN, Geens R, Zahedifard F, Stijlemans B, Zoltner M, Magez S, Sterckx YG, Zoll S
RgGuinier 3.2 nm
Dmax 11.1 nm
VolumePorod 85 nm3