Small Angle Scattering Biological Data Bank SASBDB

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SASDUN7 – Lcl C-terminal domain K520A mutant

Sample:	HbP1 (K520A) trimer, 56 kDa Legionella pneumophila protein
Buffer:	20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Jul 29

The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans. Nat Commun 15(1):4912 (2024)
Rehman S, Antonovic AK, McIntire IE, Zheng H, Cleaver L, Baczynska M, Adams CO, Portlock T, Richardson K, Shaw R, Oregioni A, Mastroianni G, Whittaker SB, Kelly G, Lorenz CD, Fornili A, Cianciotto NP, Garnett JA

R_g^Guinier	2.8	nm
D_max	9.8	nm
Volume^Porod	100	nm³

SASDUP7 – Lcl C-terminal domain D521A mutant

Sample:	HbP1 (D521A) trimer, 56 kDa Legionella pneumophila protein
Buffer:	20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Jul 29

R_g^Guinier	2.8	nm
D_max	9.8	nm
Volume^Porod	108	nm³

SASDUQ7 – Lcl C-terminal domain K526A mutant

Sample:	HbP1 (K526A) trimer, 56 kDa Legionella pneumophila protein
Buffer:	20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Jul 29

R_g^Guinier	2.8	nm
D_max	9.5	nm
Volume^Porod	104	nm³

SASDPC4 – Complex of Rhodopseudomonas palustris pAgo with TIR-like effector protein

TIR domain-containing proteinUncharacterized protein (PIWI) experimental SAS data

Sample:	TIR domain-containing protein monomer, 57 kDa Rhodopseudomonas palustris (strain … protein Uncharacterized protein (PIWI) monomer, 56 kDa Rhodopseudomonas palustris (strain … protein
Buffer:	20 mM Tris-HCl, pH8.0, 200 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Sep 27

Complex of Rhodopseudomonas palustris pAgo with TIR-like effector protein
Elena Manakova

R_g^Guinier	3.6	nm
D_max	12.0	nm
Volume^Porod	192	nm³

SASDSP7 – 5’-terminal stem-loop 1 RNA element of SARS-CoV-2 (5_SL1)

Sample:	5_SL1 monomer, 9 kDa Severe acute respiratory … RNA
Buffer:	50 mM BisTris, 25 mM NaCl, pH: 6.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Dec 1

The 5'-terminal stem-loop RNA element of SARS-CoV-2 features highly dynamic structural elements that are sensitive to differences in cellular pH. Nucleic Acids Res (2024)
Toews S, Wacker A, Faison EM, Duchardt-Ferner E, Richter C, Mathieu D, Bottaro S, Zhang Q, Schwalbe H

R_g^Guinier	1.7	nm
D_max	5.5	nm
Volume^Porod	16	nm³

SASDSM9 – Hen egg white lysozyme in 1 mol% ethylammonium nitrate

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	1 mol% ethylammonium nitrate, pH: 8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 27

Scattering approaches to unravel protein solution behaviors in ionic liquids and deep eutectic solvents: From basic principles to recent developments Advances in Colloid and Interface Science :103242 (2024)
Han Q, Veríssimo N, Bryant S, Martin A, Huang Y, Pereira J, Santos-Ebinuma V, Zhai J, Bryant G, Drummond C, Greaves T

R_g^Guinier	1.6	nm
D_max	6.2	nm
Volume^Porod	20	nm³

SASDSN9 – Buffer over-subtracted hen egg white lysozyme in 1 mol% ethylammonium nitrate

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	1 mol% ethylammonium nitrate, pH: 8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 27

R_g^Guinier	1.6	nm
D_max	6.2	nm
Volume^Porod	28	nm³

SASDSP9 – Buffer under-substracted hen egg white lysozyme in 1mol% ethylammonium nitrate

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	1 mol% ethylammonium nitrate, pH: 8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 27

R_g^Guinier	1.6	nm
D_max	6.2	nm
Volume^Porod	23	nm³

SASDSW8 – Apo form of vaccinia virus polymerase holoenzyme E9-A20-D4

DNA polymeraseDNA polymerase processivity factor component OPG148Uracil-DNA glycosylase experimental SAS data

DNA polymerase DNA polymerase processivity factor component OPG148 Uracil-DNA glycosylase Kratky plot

Sample:	DNA polymerase monomer, 117 kDa Vaccinia virus (strain … protein DNA polymerase processivity factor component OPG148 monomer, 49 kDa Vaccinia virus (strain … protein Uracil-DNA glycosylase monomer, 27 kDa Vaccinia virus (strain … protein
Buffer:	200 mM NaCl, 20 mM Tris-HCl and 1 mM TCEP, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jan 25

Structure and flexibility of the DNA polymerase holoenzyme of vaccinia virus. PLoS Pathog 20(5):e1011652 (2024)
Burmeister WP, Boutin L, Balestra AC, Gröger H, Ballandras-Colas A, Hutin S, Kraft C, Grimm C, Böttcher B, Fischer U, Tarbouriech N, Iseni F

R_g^Guinier	5.0	nm
D_max	18.0	nm
Volume^Porod	350	nm³

SASDU93 – multimodular GH16_3 containing a CBM and a catalytic domain

Glycosyl hydrolase, family 16 experimental SAS data

Sample:	Glycosyl hydrolase, family 16 monomer, 58 kDa Christiangramia forsetii (strain … protein
Buffer:	10 mM MOPS, 100 mM NaCl, pH: 7.8
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Dec 1

Unveiling the role of novel carbohydrate-binding modules in laminarin interaction of multimodular proteins from marine Bacteroidota during phytoplankton blooms. Environ Microbiol 26(5):e16624 (2024)
Zühlke MK, Ficko-Blean E, Bartosik D, Terrapon N, Jeudy A, Jam M, Wang F, Welsch N, Dürwald A, Martin LT, Larocque R, Jouanneau D, Eisenack T, Thomas F, Trautwein-Schult A, Teeling H, Becher D, Schweder T, Czjzek M

R_g^Guinier	3.8	nm
D_max	12.9	nm
Volume^Porod	76	nm³

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