Small Angle Scattering Biological Data Bank SASBDB

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SASDTN9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 10 µs post temperature jump (44 °C - 50 °C)

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30

Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
Bennett AL, Edwards R, Kosheleva I, Saunders C, Bililign Y, Williams A, Bubphamala P, Manosouri K, Anasti K, Saunders KO, Alam SM, Haynes BF, Acharya P, Henderson R

R_g^Guinier	5.3	nm
D_max	15.1	nm
Volume^Porod	750	nm³

SASDTP9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 50 µs post temperature jump (44 °C - 50 °C)

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30

R_g^Guinier	5.3	nm
D_max	15.1	nm
Volume^Porod	752	nm³

SASDTQ9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 100 µs post temperature jump (44 °C - 50 °C)

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30

R_g^Guinier	5.3	nm
D_max	15.1	nm
Volume^Porod	755	nm³

SASDTR9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 250 µs post temperature jump (44 °C - 50 °C)

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30

R_g^Guinier	5.3	nm
D_max	15.3	nm
Volume^Porod	741	nm³

SASDTS9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 500 µs post temperature jump (44 °C - 50 °C)

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30

R_g^Guinier	5.3	nm
D_max	15.1	nm
Volume^Porod	750	nm³

SASDTT9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 750 µs post temperature jump (44 °C - 50 °C)

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30

R_g^Guinier	5.3	nm
D_max	15.3	nm
Volume^Porod	739	nm³

SASDTU9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 1 ms post temperature jump (44 °C - 50 °C)

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30

R_g^Guinier	5.3	nm
D_max	15.1	nm
Volume^Porod	751	nm³

SASDTV9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 10 ms post temperature jump (44 °C - 50 °C)

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30

R_g^Guinier	5.3	nm
D_max	15.5	nm
Volume^Porod	733	nm³

SASDTW9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 100 ms post temperature jump (44 °C - 50 °C)

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30

R_g^Guinier	5.3	nm
D_max	15.3	nm
Volume^Porod	732	nm³

SASDTX9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate including the 4.1 stabilization mutations at 25 °C

CH505TF.6R.SOSIP.664.v4.1 Env glycoprotein experimental SAS data

CH505TF.6R.SOSIP.664.v4.1 Env glycoprotein Kratky plot

Sample:	CH505TF.6R.SOSIP.664.v4.1 Env glycoprotein trimer, 218 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1

R_g^Guinier	5.1	nm
D_max	18.2	nm
Volume^Porod	718	nm³

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