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SASDRY6 – lantibiotic dehydratase MadB bound to truncated maddinglicin with a full leader peptide (Leader3)

Thiopeptide-type bacteriocin biosynthesis domain containing proteinLantibiotic, gallidermin/nisin family experimental SAS data
GASBOR model
Sample: Thiopeptide-type bacteriocin biosynthesis domain containing protein dimer, 250 kDa Clostridium sp. Maddingley … protein
Lantibiotic, gallidermin/nisin family monomer, 3 kDa Clostridium sp. Maddingley … protein
Buffer: 50 mM HEPES, 200 mM NaCl, 1 % (v/v) Glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 3
Structural insights into the substrate binding mechanism of the class I dehydratase MadB Communications Biology 8(1) (2025)
Knospe C, Ortiz J, Reiners J, Kedrov A, Gertzen C, Smits S, Schmitt L
RgGuinier 4.5 nm
Dmax 13.8 nm
VolumePorod 445 nm3

SASDPT5 – Penicilin-Binding Protein 5 - PBP5∆1-39

Penicillin-Binding Protein 5 - PBP5∆1-39 experimental SAS data
DAMMIF model
Sample: Penicillin-Binding Protein 5 - PBP5∆1-39 monomer, 75 kDa Clostridioides difficile protein
Buffer: 100mM Tris·HCl, 150mM NaCl, 1mM EDTA, 1mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
SAXS of the Penicillin-Binding Protein 5 from Clostridioides difficile
Guillem Hernandez
RgGuinier 3.8 nm
Dmax 15.0 nm
VolumePorod 109 nm3

SASDPU5 – Murine hepatitis virus (MHV) coronavirus putative packaging signal RNA

MHV coronavirus putative packaging signal RNA experimental SAS data
MHV coronavirus putative packaging signal RNA Kratky plot
Sample: MHV coronavirus putative packaging signal RNA monomer, 31 kDa Murine coronavirus RNA
Buffer: 70 mM KCl, 50 μM EDTA, 25 mM HEPES, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2021 Jun 10
MHV Coronavirus Packaging Signal RNA and N protein
Suzette Pabit
RgGuinier 3.9 nm
Dmax 12.0 nm
VolumePorod 58 nm3

SASDPV5 – Murine hepatitis virus (MHV) coronavirus putative packaging signal RNA (SEC-SAXS)

MHV coronavirus putative packaging signal RNA experimental SAS data
MHV coronavirus putative packaging signal RNA Kratky plot
Sample: MHV coronavirus putative packaging signal RNA monomer, 31 kDa Murine coronavirus RNA
Buffer: 70 mM KCl, 50 μM EDTA, 25 mM HEPES, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jul 16
MHV Coronavirus Packaging Signal RNA and N protein
Suzette Pabit
RgGuinier 3.9 nm
Dmax 12.3 nm
VolumePorod 55 nm3

SASDPW5 – Murine hepatitis virus (MHV) coronavirus putative packaging signal RNA (SAXS/WAXS)

MHV coronavirus putative packaging signal RNA experimental SAS data
MHV coronavirus putative packaging signal RNA Kratky plot
Sample: MHV coronavirus putative packaging signal RNA monomer, 31 kDa Murine coronavirus RNA
Buffer: 50 mM NaCl, 50 μM EDTA, 10 mM MOPS, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2020 Sep 10
MHV Coronavirus Packaging Signal RNA and N protein
Suzette Pabit
RgGuinier 3.8 nm
Dmax 13.3 nm
VolumePorod 58 nm3

SASDPX5 – Murine hepatitis virus (MHV) coronavirus nucleocapsid protein N

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein monomer, 50 kDa Murine coronavirus (strain … protein
Buffer: 70 mM KCl, 50 μM EDTA, 25 mM HEPES, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2021 Jun 10
MHV Coronavirus Packaging Signal RNA and N protein
Suzette Pabit
RgGuinier 5.1 nm
Dmax 11.9 nm
VolumePorod 91 nm3

SASDQY7 – StyI glutathione S-transferase

GST N-terminal domain-containing protein experimental SAS data
CORAL model
Sample: GST N-terminal domain-containing protein dimer, 60 kDa Gordonia rubripertincta protein
Buffer: 10 mM Tris, 300 mM NaCl,, pH: 8
Experiment: SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2022 Jan 20
Glutathione S -Transferase Mediated Epoxide Conversion: Functional and Structural Properties of an Enantioselective Catalyst ACS Catalysis :12308-12324 (2025)
Haarmann M, Scholz M, Lienkamp A, Burnik J, Justesen B, Reiners J, Maier A, Eggerichs D, Vocke K, Smits S, Günther Pomorski T, Hofmann E, Tischler D
RgGuinier 2.7 nm
Dmax 9.2 nm
VolumePorod 91 nm3

SASDW94 – Horse apoferritin from AF4-SAXS measurements

Ferritin light chain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ferritin light chain 24-mer, 480 kDa Equus caballus protein
Buffer: phosphate buffered saline, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19
AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline. J Synchrotron Radiat (2025)
Da Vela S, Bartels K, Franke D, Soloviov D, Gräwert T, Molodenskiy D, Kolb B, Wilhelmy C, Drexel R, Meier F, Haas H, Langguth P, Graewert MA
RgGuinier 5.4 nm
Dmax 13.3 nm
VolumePorod 632 nm3

SASDWB4 – Sweet potato beta-amylase from AF4-SAXS measurement

Beta-amylase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Beta-amylase tetramer, 224 kDa Ipomoea batatas protein
Buffer: phosphate buffered saline, 1% glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Apr 13
AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline. J Synchrotron Radiat (2025)
Da Vela S, Bartels K, Franke D, Soloviov D, Gräwert T, Molodenskiy D, Kolb B, Wilhelmy C, Drexel R, Meier F, Haas H, Langguth P, Graewert MA
RgGuinier 4.2 nm
Dmax 12.6 nm
VolumePorod 297 nm3

SASDX42 – IgA peptidase (A31-I1166; active site mutant E540A)

IgA protease (E540A) experimental SAS data
IgA peptidase (A31-I1166; active site mutant E540A) Rg histogram
Sample: IgA protease (E540A) monomer, 128 kDa Thomasclavelia ramosa protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 23
Biochemical and structural characterization of the human gut microbiome metallopeptidase IgAse provides insight into its unique specificity for the Fab' region of IgA1 and IgA2. PLoS Pathog 21(7):e1013292 (2025)
Ramírez-Larrota JS, Juyoux P, Guerra P, Eckhard U, Gomis-Rüth FX
RgGuinier 5.7 nm
Dmax 27.9 nm
VolumePorod 190 nm3