Small Angle Scattering Biological Data Bank SASBDB

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SASDTC9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 500 µs post temperature jump (44 °C - 50 °C)

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data

CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28

Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
Bennett AL, Edwards R, Kosheleva I, Saunders C, Bililign Y, Williams A, Bubphamala P, Manosouri K, Anasti K, Saunders KO, Alam SM, Haynes BF, Acharya P, Henderson R

R_g^Guinier	4.3	nm
D_max	15.3	nm
Volume^Porod	728	nm³

SASDTD9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 1 ms (20 Hz) post temperature jump (44 °C - 50 °C)

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28

R_g^Guinier	4.3	nm
D_max	15.3	nm
Volume^Porod	742	nm³

SASDTE9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 1 ms (5 Hz) post temperature jump (44 °C - 50 °C)

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 29

R_g^Guinier	4.1	nm
D_max	15.4	nm
Volume^Porod	706	nm³

SASDTF9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 10 ms post temperature jump (44 °C - 50 °C)

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 29

R_g^Guinier	4.1	nm
D_max	15.4	nm
Volume^Porod	706	nm³

SASDTG9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 100 ms post temperature jump (44 °C - 50 °C)

Sample:	CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 29

R_g^Guinier	4.1	nm
D_max	15.5	nm
Volume^Porod	706	nm³

SASDTH9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate at 30 °C

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 28

R_g^Guinier	4.1	nm
D_max	15.3	nm
Volume^Porod	768	nm³

SASDTJ9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate at 35 °C

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 28

R_g^Guinier	5.3	nm
D_max	15.3	nm
Volume^Porod	765	nm³

SASDTK9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate at 40 °C

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 28

R_g^Guinier	5.3	nm
D_max	15.3	nm
Volume^Porod	764	nm³

SASDTL9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate at 44 °C

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 28

R_g^Guinier	5.2	nm
D_max	15.2	nm
Volume^Porod	728	nm³

SASDTM9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 5 µs post temperature jump (44 °C - 50 °C)

Sample:	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer:	15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment:	SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30

R_g^Guinier	5.3	nm
D_max	15.1	nm
Volume^Porod	750	nm³

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