Small Angle Scattering Biological Data Bank SASBDB

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SASDRV4 – Mycobacterium marinum phosphoserine phosphatase (MmSerB2) dimer

phosphoserine phosphatase experimental SAS data

Sample:	Phosphoserine phosphatase dimer, 88 kDa Mycobacterium marinum (strain … protein
Buffer:	50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2021 Oct 6

A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J

R_g^Guinier	3.2	nm
D_max	9.9	nm
Volume^Porod	138	nm³

SASDRW4 – Mycobacterium avium phosphoserine phosphatase (MaSerB) dimer

Phosphoserine phosphatase (G31R, G152E) experimental SAS data

Sample:	Phosphoserine phosphatase (G31R, G152E) dimer, 88 kDa Mycobacterium avium (strain … protein
Buffer:	50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2021 Oct 6

A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J

R_g^Guinier	3.3	nm
D_max	10.1	nm
Volume^Porod	138	nm³

SASDLC7 – Polyribonucleotide nucleotidyltransferase (PNPase): apo form

Polyribonucleotide nucleotidyltransferase experimental SAS data

Sample:	Polyribonucleotide nucleotidyltransferase trimer, 246 kDa Campylobacter jejuni subsp. … protein
Buffer:	20 mM Tris-HCl, 10 mM NAH2PO4, 60 mM KCl, 1 mM MgCl2, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Mar 17

Structure and function of Campylobacter jejuni polynucleotide phosphorylase (PNPase): Insights into the role of this RNase in pathogenicity. Biochimie (2023)
Bárria C, Athayde D, Hernandez G, Fonseca L, Casinhas J, Cordeiro TN, Archer M, Arraiano CM, Brito JA, Matos RG

R_g^Guinier	3.9	nm
D_max	11.0	nm
Volume^Porod	310	nm³

SASDLD7 – Polyribonucleotide nucleotidyltransferase (PNPase): 2-AMP complex

Sample:	Polyribonucleotide nucleotidyltransferase trimer, 237 kDa Campylobacter jejuni subsp. … protein
Buffer:	20 mM Tris.HCl, 10 mM NAH2PO4, 60 mM KCl, 1 mM MgCl2, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Mar 17

R_g^Guinier	3.9	nm
D_max	11.0	nm
Volume^Porod	315	nm³

SASDLE7 – Polyribonucleotide nucleotidyltransferase (PNPase): 2-GMP complex

Sample:	Polyribonucleotide nucleotidyltransferase trimer, 237 kDa Campylobacter jejuni subsp. … protein
Buffer:	20 mM Tris.HCl, 10 mM NAH2PO4, 60 mM KCl, 1mM MgCl2, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Mar 17

R_g^Guinier	3.9	nm
D_max	11.0	nm
Volume^Porod	312	nm³

SASDRV7 – SARS-CoV-2 5' untranslated region stem-loop 4 RNA (5_SL4) in phosphate buffer

stem-loop 4 from SARS-CoV-2 5'-UTR experimental SAS data

stem-loop 4 from SARS-CoV-2 5'-UTR Kratky plot

Sample:	Stem-loop 4 from SARS-CoV-2 5'-UTR monomer, 14 kDa SARS coronavirus 2-Wuhan RNA
Buffer:	25 mM potassium phosphate pH 6.5, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7

High-resolution structure of stem-loop 4 from the 5'-UTR of SARS-CoV-2 solved by solution state NMR. Nucleic Acids Res (2023)
Vögele J, Hymon D, Martins J, Ferner J, Jonker HRA, Hargrove AE, Weigand JE, Wacker A, Schwalbe H, Wöhnert J, Duchardt-Ferner E

R_g^Guinier	2.1	nm
D_max	7.0	nm
Volume^Porod	22	nm³

SASDR25 – Periplasmic domain of cholera toxin transcriptional activator ToxR

Cholera toxin transcriptional activator experimental SAS data

Sample:	Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Buffer:	50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6

Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T

R_g^Guinier	1.6	nm
D_max	6.0	nm
Volume^Porod	16	nm³

SASDR35 – Periplasmic domain of cholera transmembrane regulatory protein ToxS

Transmembrane regulatory protein ToxS experimental SAS data

Sample:	Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
Buffer:	50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6

R_g^Guinier	2.3	nm
D_max	7.4	nm
Volume^Porod	55	nm³

SASDR45 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS

Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxS experimental SAS data

Sample:	Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
Buffer:	50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6

R_g^Guinier	2.1	nm
D_max	6.6	nm
Volume^Porod	51	nm³

SASDR55 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS bound to bile salt

Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxSbile acid: sodium cholate hydrate experimental SAS data

Sample:	Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein Bile acid: sodium cholate hydrate monomer, 0 kDa
Buffer:	50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Jul 7

R_g^Guinier	2.4	nm
D_max	6.6	nm
Volume^Porod	60	nm³

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