Small Angle Scattering Biological Data Bank SASBDB

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SASDV52 – Aspergillus niger beta-glucosidase (bgl1), native, glycosylated

Sample:	Beta-glucosidase dimer, 187 kDa Aspergillus niger protein
Buffer:	50 mM sodium acetate, pH: 5
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jun 4

Aspergillus niger beta-glucosidase (bgl1)
Estella Yee

R_g^Guinier	4.4	nm
D_max	13.9	nm
Volume^Porod	277	nm³

SASDT75 – Trypanosoma brucei gambiense invariant surface glycoprotein 43 (ISG43)

T. brucei spp.-specific protein experimental SAS data

Sample:	T. brucei spp.-specific protein monomer, 38 kDa Trypanosoma brucei gambiense … protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
Sülzen H, Volkov AN, Geens R, Zahedifard F, Stijlemans B, Zoltner M, Magez S, Sterckx YG, Zoll S

R_g^Guinier	3.2	nm
D_max	11.5	nm
Volume^Porod	53	nm³

SASDT85 – Trypanosoma brucei gambiense invariant surface glycoprotein 64 (ISG64)

64 kDa invariant surface glycoprotein, putative experimental SAS data

Sample:	64 kDa invariant surface glycoprotein, putative monomer, 40 kDa Trypanosoma brucei gambiense … protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

R_g^Guinier	3.1	nm
D_max	11.2	nm
Volume^Porod	56	nm³

SASDT95 – Trypanosoma brucei gambiense invariant surface glycoprotein 75 (ISG75)

Sample:	ISG75 monomer, 50 kDa Trypanosoma brucei gambiense protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Nov 25

R_g^Guinier	3.2	nm
D_max	11.1	nm
Volume^Porod	85	nm³

SASDTA5 – Trypanosoma brucei gambiense variable surface glycoprotein LiTat 1.5

Variable surface glycoprotein LiTat 1.5 experimental SAS data

Sample:	Variable surface glycoprotein LiTat 1.5 dimer, 98 kDa Trypanosoma brucei gambiense protein
Buffer:	50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

R_g^Guinier	4.4	nm
D_max	17.2	nm
Volume^Porod	170	nm³

SASDTB5 – Trypanosoma brucei gambiense variant surface glycoprotein 3.1

Variant surface glycoprotein 3.1 experimental SAS data

Sample:	Variant surface glycoprotein 3.1 dimer, 103 kDa Trypanosoma brucei gambiense protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

R_g^Guinier	4.7	nm
D_max	17.0	nm
Volume^Porod	149	nm³

SASDS56 – Nucleotide binding domain, NBD (ABC transporter domain) from the RTX toxin ABC transporter RtxB

RTX toxin transporter experimental SAS data

Sample:	RTX toxin transporter monomer, 28 kDa Kingella kingae ATCC … protein
Buffer:	100 mM HEPES, 10 % glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20

Type 1 secretion necessitates a tight interplay between all domains of the ABC transporter Scientific Reports 14(1) (2024)
Anlauf M, Bilsing F, Reiners J, Spitz O, Hachani E, Smits S, Schmitt L

R_g^Guinier	2.2	nm
D_max	7.6	nm
Volume^Porod	48	nm³

SASDQS9 – A ssr1698 Dri1 hemoprotein, H21A variant + heme

Ssr1698 protein (H21A) experimental SAS data

Sample:	Ssr1698 protein (H21A) dimer, 22 kDa Synechocystis sp. (strain … protein
Buffer:	50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Dec 7

A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE

R_g^Guinier	2.0	nm
D_max	6.5	nm
Volume^Porod	28	nm³

SASDRD5 – A ssr1698 Dri1 hemoprotein, H79A:R90A variant + heme

Ssr1698 protein (H79A:R90A) experimental SAS data

Sample:	Ssr1698 protein (H79A:R90A) dimer, 22 kDa Synechocystis sp. (strain … protein
Buffer:	50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Mar 30

R_g^Guinier	2.0	nm
D_max	6.3	nm
Volume^Porod	28	nm³

SASDRE5 – A ssr1698 Dri1 hemoprotein, H79A:R90A variant

Sample:	Ssr1698 protein (H79A:R90A) monomer, 11 kDa Synechocystis sp. (strain … protein
Buffer:	50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Mar 30

R_g^Guinier	1.6	nm
D_max	5.5	nm
Volume^Porod	12	nm³

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