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SASDTU7 – Lactoferrin binding protein B (C-terminal alpha helical domain)

Transferrin-binding protein B experimental SAS data
Transferrin-binding protein B Kratky plot
Sample: Transferrin-binding protein B monomer, 32 kDa Moraxella bovis protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 23
A secreted bacterial protein protects bacteria from cationic antimicrobial peptides by entrapment in phase separated droplets PNAS Nexus (2024)
Ostan N, Cole G, Wang F, Reichheld S, Moore G, Pan C, Yu R, Lai C, Sharpe S, Lee H, Schryvers A, Moraes T
RgGuinier 3.2 nm
Dmax 11.9 nm
VolumePorod 49 nm3

SASDUS3 – Dark-adapted photocobilin diguanylate cyclase fusion from Acidimicrobiaceae bacterium

GGDEF domain-containing protein experimental SAS data
DAMMIF model
Sample: GGDEF domain-containing protein dimer, 132 kDa Acidimicrobiaceae bacterium protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jan 21
Photocobilins integrate B(12) and bilin photochemistry for enzyme control. Nat Commun 15(1):2740 (2024)
Zhang S, Jeffreys LN, Poddar H, Yu Y, Liu C, Patel K, Johannissen LO, Zhu L, Cliff MJ, Yan C, Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS
RgGuinier 4.1 nm
Dmax 14.9 nm
VolumePorod 194 nm3

SASDUT3 – Light-adapted photocobilin diguanylate cyclase fusion from Acidimicrobiaceae bacterium

GGDEF domain-containing protein experimental SAS data
DAMMIF model
Sample: GGDEF domain-containing protein dimer, 132 kDa Acidimicrobiaceae bacterium protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jan 21
Photocobilins integrate B(12) and bilin photochemistry for enzyme control. Nat Commun 15(1):2740 (2024)
Zhang S, Jeffreys LN, Poddar H, Yu Y, Liu C, Patel K, Johannissen LO, Zhu L, Cliff MJ, Yan C, Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS
RgGuinier 5.0 nm
Dmax 18.5 nm
VolumePorod 170 nm3

SASDUG3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) monomer

Endoglucanase-7 experimental SAS data
Endoglucanase-7 Kratky plot
Sample: Endoglucanase-7 monomer, 30 kDa Colletotrichum orbiculare (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 1
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 3.0 nm
Dmax 12.0 nm
VolumePorod 50 nm3

SASDUH3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) dimer

Endoglucanase-7 experimental SAS data
Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) dimer Rg histogram
Sample: Endoglucanase-7 dimer, 60 kDa Colletotrichum orbiculare (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 1
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 4.8 nm
Dmax 21.7 nm
VolumePorod 83 nm3

SASDUJ3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) deltaX283

Endoglucanase-7 experimental SAS data
Endoglucanase-7 Kratky plot
Sample: Endoglucanase-7 monomer, 27 kDa Colletotrichum orbiculare (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Feb 1
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 1.8 nm
Dmax 6.3 nm
VolumePorod 32 nm3

SASDUK3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) C286A*

Endoglucanase-7 (C286A) experimental SAS data
Endoglucanase-7 (C286A) Kratky plot
Sample: Endoglucanase-7 (C286A) monomer, 30 kDa Colletotrichum orbiculare (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Feb 1
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 3.0 nm
Dmax 11.6 nm
VolumePorod 48 nm3

SASDUL3 – Colletotrichum higginsianum Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) monomer

Glycosyl hydrolase family 61 experimental SAS data
Glycosyl hydrolase family 61 Kratky plot
Sample: Glycosyl hydrolase family 61 monomer, 30 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 9
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 4.1 nm
Dmax 14.6 nm
VolumePorod 85 nm3

SASDUM3 – Colletotrichum higginsianum Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) dimer

Glycosyl hydrolase family 61 experimental SAS data
Glycosyl hydrolase family 61 Kratky plot
Sample: Glycosyl hydrolase family 61 dimer, 61 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 9
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 6.2 nm
Dmax 24.5 nm
VolumePorod 206 nm3

SASDUN3 – Colletotrichum higginsianum Auxiliary Activity Family 9B Lytic Polysaccharide Monooxygenase (AA9B LPMO) monomer

Endoglucanase-4 experimental SAS data
Endoglucanase-4 Kratky plot
Sample: Endoglucanase-4 monomer, 33 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Feb 17
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 3.9 nm
Dmax 17.0 nm
VolumePorod 68 nm3