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SASDS99 – Full length SARS Non-structural protein 10 (Nsp10)

Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 16 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 6
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.9 nm
VolumePorod 19 nm3

SASDSA9 – Full length MERS Non-structural protein 10 (Nsp10)

Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Middle East respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 6
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.6 nm
VolumePorod 19 nm3

SASDSU6 – Palivizumab IgG

Palivizumab IgG experimental SAS data
CORAL model
Sample: Palivizumab IgG monomer, 145 kDa Commercial (Synagis) protein
Buffer: PBS (137 mM NaCl, 2.7 mM KCl, 12 mM HPO4 2−/H2PO4 −), pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14
Respiratory syncytial virus (RSV)-approved monoclonal antibody Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors Journal of Biological Chemistry :105270 (2023)
Ettich J, Wittich C, Moll J, Behnke K, Floss D, Reiners J, Christmann A, Lang P, Smits S, Kolmar H, Scheller J
RgGuinier 5.0 nm
Dmax 16.8 nm
VolumePorod 252 nm3

SASDSV6 – Nanobody AIP1 VHH

Anti-Idiotypic-Palivizumab-Nanobody1 experimental SAS data
CORAL model
Sample: Anti-Idiotypic-Palivizumab-Nanobody1 monomer, 20 kDa Lama glama protein
Buffer: PBS (137 mM NaCl, 2.7 mM KCl, 12 mM HPO4 2−/H2PO4 −), pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14
Respiratory syncytial virus (RSV)-approved monoclonal antibody Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors Journal of Biological Chemistry :105270 (2023)
Ettich J, Wittich C, Moll J, Behnke K, Floss D, Reiners J, Christmann A, Lang P, Smits S, Kolmar H, Scheller J
RgGuinier 2.6 nm
Dmax 8.8 nm
VolumePorod 31 nm3

SASDSW6 – Palivizumab Nanobody AIP1VHH complex

Palivizumab IgGAnti-Idiotypic-Palivizumab-Nanobody1 experimental SAS data
CORAL model
Sample: Palivizumab IgG monomer, 145 kDa Commercial (Synagis) protein
Anti-Idiotypic-Palivizumab-Nanobody1 monomer, 20 kDa Lama glama protein
Buffer: PBS (137 mM NaCl, 2.7 mM KCl, 12 mM HPO4 2−/H2PO4 −), pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14
Respiratory syncytial virus (RSV)-approved monoclonal antibody Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors Journal of Biological Chemistry :105270 (2023)
Ettich J, Wittich C, Moll J, Behnke K, Floss D, Reiners J, Christmann A, Lang P, Smits S, Kolmar H, Scheller J
RgGuinier 6.0 nm
Dmax 20.4 nm

SASDJ67 – SAM2 Riboswitch 20 mM NaCl

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 3.5 nm

SASDJ77 – SAM2 Riboswitch 50 mM NaCl

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 50 mM NaCl, 20 μM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 3.3 nm

SASDJ87 – SAM2 Riboswitch 100 mM NaCl

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 100 mM NaCl, 20 μM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 3.0 nm

SASDJ97 – SAM2 Riboswitch 200 mM NaCl

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 200 mM NaCl, 20μM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 3.0 nm

SASDJA7 – SAM2 Riboswitch 0.5 mM MgCl2

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 0.5 mM MgCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 3.2 nm