Small Angle Scattering Biological Data Bank SASBDB

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SASDPZ5 – Gcf1p protein bound to DNA at 1.0 mg/mL

Sample:	Gcf1p monomer, 26 kDa Candida albicans (strain … protein Af2_20 DNA monomer, 12 kDa DNA
Buffer:	25 mM Tris, 20 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Aug 30

Structural analysis of the Candida albicans mitochondrial DNA maintenance factor Gcf1p reveals a dynamic DNA-bridging mechanism. Nucleic Acids Res (2023)
Tarrés-Solé A, Battistini F, Gerhold JM, Piétrement O, Martínez-García B, Ruiz-López E, Lyonnais S, Bernadó P, Roca J, Orozco M, Le Cam E, Sedman J, Solà M

R_g^Guinier	3.6	nm
D_max	16.0	nm
Volume^Porod	92	nm³

SASDP26 – Gcf1p protein bound to DNA at 2.0 mg/mL

Sample:	Gcf1p monomer, 26 kDa Candida albicans (strain … protein Af2_20 DNA monomer, 12 kDa DNA
Buffer:	25 mM Tris, 20 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Aug 30

R_g^Guinier	3.9	nm
D_max	16.0	nm
Volume^Porod	133	nm³

SASDP36 – N-terminal truncated Gcf1p (amino acids 59-245) bound to DNA

Af2_20 DNAGcf1p(Δ58) experimental SAS data

Sample:	Af2_20 DNA monomer, 12 kDa DNA Gcf1p(Δ58) monomer, 22 kDa Candida albicans (strain … protein
Buffer:	25 mM Tris, 20 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Aug 30

R_g^Guinier	2.6	nm
D_max	10.5	nm
Volume^Porod	52	nm³

SASDQJ6 – Monkeypox viral synthetic DNA fragment (DNA sequence 1, MP1)

Monekypox DNA sequence 1 experimental SAS data

Sample:	Monekypox DNA sequence 1 monomer, 6 kDa Monkeypox virus DNA
Buffer:	20 mM HEPES, 100 mM KCl, and 0.2 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Sep 23

Mapping and characterization of G‐quadruplexes in monkeypox genomes Journal of Medical Virology 95(5) (2023)
Pereira H, Gemmill D, Siddiqui M, Vasudeva G, Patel T

R_g^Guinier	1.7	nm
D_max	4.4	nm
Volume^Porod	14	nm³

SASDQK6 – Monkeypox viral synthetic DNA fragment (DNA sequence 2, MP2)

Sample:	Monekypox DNA sequence 1 monomer, 6 kDa Monkeypox virus DNA
Buffer:	20 mM HEPES, 100 mM KCl, and 0.2 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Sep 23

Mapping and characterization of G‐quadruplexes in monkeypox genomes Journal of Medical Virology 95(5) (2023)
Pereira H, Gemmill D, Siddiqui M, Vasudeva G, Patel T

R_g^Guinier	1.6	nm
D_max	4.5	nm
Volume^Porod	11	nm³

SASDQL6 – Monkeypox viral synthetic DNA fragment (DNA sequence 1, MP1 mutant)

Monekypox DNA sequence 1 mutant experimental SAS data

Sample:	Monekypox DNA sequence 1 mutant monomer, 6 kDa Monkeypox virus DNA
Buffer:	20 mM HEPES, 100 mM KCl, and 0.2 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Sep 23

Mapping and characterization of G‐quadruplexes in monkeypox genomes Journal of Medical Virology 95(5) (2023)
Pereira H, Gemmill D, Siddiqui M, Vasudeva G, Patel T

R_g^Guinier	1.9	nm
D_max	5.5	nm
Volume^Porod	16	nm³

SASDQM6 – Monkeypox viral synthetic DNA fragment (DNA sequence 2, MP2 mutant)

Monekypox DNA sequence 2 mutant experimental SAS data

Sample:	Monekypox DNA sequence 2 mutant monomer, 6 kDa Monkeypox virus DNA
Buffer:	20 mM HEPES, 100 mM KCl, and 0.2 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Sep 23

Mapping and characterization of G‐quadruplexes in monkeypox genomes Journal of Medical Virology 95(5) (2023)
Pereira H, Gemmill D, Siddiqui M, Vasudeva G, Patel T

R_g^Guinier	1.9	nm
D_max	6.0	nm
Volume^Porod	8	nm³

SASDRR4 – Diffusive GAC rRNA + Mg: Equilibrium final

50S ribosomal protein L11 experimental SAS data

Sample:	50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L

R_g^Guinier	2.2	nm
D_max	11.0	nm
Volume^Porod	27	nm³

SASDRG8 – Astaxanthin-binding protein orange1 (AstaPo1) complexed with zeaxanthin

Astaxanthin binding fasciclin family protein experimental SAS data

Sample:	Astaxanthin binding fasciclin family protein monomer, 22 kDa Coelastrella astaxanthina protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2021 Sep 24

Structural basis for the ligand promiscuity of the neofunctionalized, carotenoid-binding fasciclin domain protein AstaP. Commun Biol 6(1):471 (2023)
Kornilov FD, Slonimskiy YB, Lunegova DA, Egorkin NA, Savitskaya AG, Kleymenov SY, Maksimov EG, Goncharuk SA, Mineev KS, Sluchanko NN

R_g^Guinier	2.1	nm
D_max	8.0	nm
Volume^Porod	43	nm³

SASDRH8 – Astaxanthin-binding protein orange1 (AstaPo1) complexed with canthaxanthin

Sample:	Astaxanthin binding fasciclin family protein monomer, 22 kDa Coelastrella astaxanthina protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2021 Sep 24

R_g^Guinier	2.1	nm
D_max	8.5	nm
Volume^Porod	46	nm³

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