Small Angle Scattering Biological Data Bank SASBDB

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SASDSU6 – Palivizumab IgG

Sample:	Palivizumab IgG monomer, 145 kDa Commercial (Synagis) protein
Buffer:	PBS (137 mM NaCl, 2.7 mM KCl, 12 mM HPO4 2−/H2PO4 −), pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14

Respiratory syncytial virus (RSV)-approved monoclonal antibody Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors Journal of Biological Chemistry :105270 (2023)
Ettich J, Wittich C, Moll J, Behnke K, Floss D, Reiners J, Christmann A, Lang P, Smits S, Kolmar H, Scheller J

R_g^Guinier	5.0	nm
D_max	16.8	nm
Volume^Porod	252	nm³

SASDSV6 – Nanobody AIP1 VHH

Anti-Idiotypic-Palivizumab-Nanobody1 experimental SAS data

Sample:	Anti-Idiotypic-Palivizumab-Nanobody1 monomer, 20 kDa Lama glama protein
Buffer:	PBS (137 mM NaCl, 2.7 mM KCl, 12 mM HPO4 2−/H2PO4 −), pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14

R_g^Guinier	2.6	nm
D_max	8.8	nm
Volume^Porod	31	nm³

SASDSW6 – Palivizumab Nanobody AIP1VHH complex

Palivizumab IgGAnti-Idiotypic-Palivizumab-Nanobody1 experimental SAS data

Sample:	Palivizumab IgG monomer, 145 kDa Commercial (Synagis) protein Anti-Idiotypic-Palivizumab-Nanobody1 monomer, 20 kDa Lama glama protein
Buffer:	PBS (137 mM NaCl, 2.7 mM KCl, 12 mM HPO4 2−/H2PO4 −), pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14

R_g^Guinier	6.0	nm
D_max	20.4	nm

SASDJ67 – SAM2 Riboswitch 20 mM NaCl

S-adenosylmethionine riboswitch II experimental SAS data

S-adenosylmethionine riboswitch II Kratky plot

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

3.5

SASDJ77 – SAM2 Riboswitch 50 mM NaCl

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 50 mM NaCl, 20 μM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

3.3

SASDJ87 – SAM2 Riboswitch 100 mM NaCl

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 100 mM NaCl, 20 μM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

3.0

SASDJ97 – SAM2 Riboswitch 200 mM NaCl

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 200 mM NaCl, 20μM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

3.0

SASDJA7 – SAM2 Riboswitch 0.5 mM MgCl2

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 0.5 mM MgCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

3.2

SASDJB7 – SAM2 Riboswitch 1 mM MgCl2

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 1mM MgCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

2.5

SASDJC7 – SAM2 Riboswitch 2 mM MgCl2

Sample:	S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer:	20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 2 mM MgCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9

SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge

R_g^Guinier

2.2

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