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SASDJB7 – SAM2 Riboswitch 1 mM MgCl2

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 1mM MgCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.5 nm

SASDJC7 – SAM2 Riboswitch 2 mM MgCl2

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.2 nm

SASDJD7 – SAM2 Riboswitch 3 mM MgCl2

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3 mM MgCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.1 nm

SASDJE7 – SAM2 Riboswitch 3.75 mM MgCl2

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3.75 mM MgCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.1 nm

SASDJF7 – SAM2 Riboswitch 0.25 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 0.25 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.7 nm

SASDJG7 – SAM2 Riboswitch 1 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 1 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDJH7 – SAM2 Riboswitch 2 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 2 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDJJ7 – SAM2 Riboswitch 3 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDJK7 – SAM2 Riboswitch 3.75 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3.75 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDSX6 – Aquifex aeolicus domain of unknown function 507 at 1 mg/mL

DUF507 family protein experimental SAS data
CUSTOM IN-HOUSE model
Sample: DUF507 family protein monomer, 22 kDa Aquifex aeolicus (strain … protein
Buffer: 20 mM Hepes pH 7.2, 400 mM NaCl, pH: 7.2
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 7
Crystal structure of domain of unknown function 507 (DUF507) reveals a new protein fold. Sci Rep 13(1):13496 (2023)
McKay CE, Cheng J, Tanner JJ
RgGuinier 2.3 nm
Dmax 9.0 nm
VolumePorod 39 nm3