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SASDRF2 – Gamma cyclodextrin - 12.5 mg/ml aqueous solution measured to 238 inverse nm

Gamma Cyclodextrin experimental SAS data
Gamma Cyclodextrin Kratky plot
Sample: Gamma Cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.7 nm

SASDQ37 – N-terminal GST-like domain of Plasmodium berghei cytosolic glutamyl-tRNA synthetase

Glutamate--tRNA ligase experimental SAS data
OLIGOMER model
Sample: Glutamate--tRNA ligase dimer, 57 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 3.0 nm
Dmax 11.5 nm
VolumePorod 96 nm3

SASDQ47 – N-terminal GST-like domain of Plasmodium berghei tRNA import protein

tRNA import protein tRIP experimental SAS data
CORAL model
Sample: TRNA import protein tRIP dimer, 49 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 2.9 nm
Dmax 11.0 nm
VolumePorod 68 nm3

SASDQ57 – The scaffold of Plasmodium berghei multi aminoacyl-tRNA synthetase complexes

Chimeric construct containing the GST-like domains of P. berghei tRNA import protein and glutamyl-tRNA synthetase experimental SAS data
CORAL model
Sample: Chimeric construct containing the GST-like domains of P. berghei tRNA import protein and glutamyl-tRNA synthetase dimer, 105 kDa Plasmodium berghei ANKA protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 3.9 nm
Dmax 14.1 nm
VolumePorod 145 nm3

SASDQ67 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, Q-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and glutaminyl-tRNA synthetase

Glutamine--tRNA ligaseGlutamate--tRNA ligasetRNA import protein tRIP experimental SAS data
CORAL model
Sample: Glutamine--tRNA ligase dimer, 46 kDa Plasmodium berghei (strain … protein
Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
TRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 4.6 nm
Dmax 17.1 nm
VolumePorod 198 nm3

SASDQ77 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, M-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and methionyl-tRNA synthetase

Methionine--tRNA ligasetRNA import protein tRIPGlutamate--tRNA ligase experimental SAS data
CORAL model
Sample: Methionine--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
TRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein
Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 4.7 nm
Dmax 18.3 nm
VolumePorod 217 nm3

SASDWA7 – Dihydroneopterin aldolase from H.pylori (HpDHNA:Pterin)

Dihydroneopterin aldolase experimental SAS data
DAMMIN model
Sample: Dihydroneopterin aldolase tetramer, 55 kDa Helicobacter pylori (strain … protein
Buffer: 25 mM Tris-HCl pH 7.5 and 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Apr 5
Structure of Helicobacter pylori dihydroneopterin aldolase suggests a fragment-based strategy for isozyme-specific inhibitor design. Curr Res Struct Biol 5:100095 (2023)
Shaw GX, Fan L, Cherry S, Shi G, Tropea JE, Ji X
RgGuinier 2.5 nm
Dmax 7.3 nm
VolumePorod 77 nm3

SASDQB7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region)

Non-structural protein V experimental SAS data
Non-structural protein V Kratky plot
Sample: Non-structural protein V monomer, 15 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
Nilsson J, Baroudi H, Gondelaud F, Pesce G, Bignon C, Ptchelkine D, Chamieh J, Cottet H, Kajava A, Longhi S
RgGuinier 3.7 nm
Dmax 19.0 nm
VolumePorod 48 nm3

SASDQC7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region) mutated YYY --> AAA

Non-structural protein V (Y211A, Y212A, Y213A mutant) experimental SAS data
Non-structural protein V (Y211A, Y212A, Y213A mutant) Kratky plot
Sample: Non-structural protein V (Y211A, Y212A, Y213A mutant) monomer, 15 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
Nilsson J, Baroudi H, Gondelaud F, Pesce G, Bignon C, Ptchelkine D, Chamieh J, Cottet H, Kajava A, Longhi S
RgGuinier 4.0 nm
Dmax 19.0 nm
VolumePorod 49 nm3

SASDQD7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region, C-terminal truncated)

Non-structural protein V experimental SAS data
Non-structural protein V Kratky plot
Sample: Non-structural protein V monomer, 9 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
Nilsson J, Baroudi H, Gondelaud F, Pesce G, Bignon C, Ptchelkine D, Chamieh J, Cottet H, Kajava A, Longhi S
RgGuinier 2.8 nm
Dmax 13.0 nm
VolumePorod 22 nm3