|
|
|
Sample: |
Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) monomer, 14 kDa Homo sapiens protein
Polyubiquitin-B monomer, 9 kDa Fukomys damarensis protein
|
Buffer: |
100 mM NaCl, pH: 6.8
|
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2023 Mar 17
|
Conformational signatures induced by ubiquitin modification in the amyloid-forming tau repeat domain.
Proc Natl Acad Sci U S A 122(15):e2425831122 (2025)
Viola G, Trivellato D, Laitaoja M, Jänis J, Felli IC, D'Onofrio M, Mollica L, Giachin G, Assfalg M
|
RgGuinier |
3.3 |
nm |
Dmax |
13.3 |
nm |
VolumePorod |
41 |
nm3 |
|
|
|
|
|
Sample: |
Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) monomer, 14 kDa Homo sapiens protein
Polyubiquitin-B monomer, 9 kDa Fukomys damarensis protein
|
Buffer: |
100 mM NaCl, pH: 6.8
|
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2023 Mar 17
|
Conformational signatures induced by ubiquitin modification in the amyloid-forming tau repeat domain.
Proc Natl Acad Sci U S A 122(15):e2425831122 (2025)
Viola G, Trivellato D, Laitaoja M, Jänis J, Felli IC, D'Onofrio M, Mollica L, Giachin G, Assfalg M
|
RgGuinier |
3.1 |
nm |
Dmax |
12.1 |
nm |
VolumePorod |
33 |
nm3 |
|
|
|
|
|
Sample: |
Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) monomer, 14 kDa Homo sapiens protein
Polyubiquitin-B monomer, 9 kDa Fukomys damarensis protein
Polyubiquitin-B monomer, 9 kDa Fukomys damarensis protein
|
Buffer: |
100 mM NaCl, pH: 6.8
|
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2023 Mar 17
|
Conformational signatures induced by ubiquitin modification in the amyloid-forming tau repeat domain.
Proc Natl Acad Sci U S A 122(15):e2425831122 (2025)
Viola G, Trivellato D, Laitaoja M, Jänis J, Felli IC, D'Onofrio M, Mollica L, Giachin G, Assfalg M
|
RgGuinier |
3.3 |
nm |
Dmax |
12.2 |
nm |
VolumePorod |
59 |
nm3 |
|
|
|
|
|
Sample: |
Human immunoglobulin gamma 2 (IgG2) SAP1.3 F(ab')2 - heavy chain C226S dimer, 52 kDa Homo sapiens protein
Human immunoglobulin gamma 2 (IgG2) SAP1.3 F(ab')2 - kappa light chain C214S dimer, 48 kDa Homo sapiens protein
|
Buffer: |
50 mM HEPES, 150 mM KCl, pH: 7.5
|
Experiment: |
SAXS
data collected at BM29, ESRF on 2022 Jun 8
|
Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.
Nat Commun 16(1):3495 (2025)
Elliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I
|
RgGuinier |
3.8 |
nm |
Dmax |
12.9 |
nm |
VolumePorod |
130 |
nm3 |
|
|
|
|
|
Sample: |
Human immunoglobulin gamma 2 (IgG2) SAP1.3 F(ab')2 - heavy chain C227S dimer, 52 kDa protein
Human immunoglobulin gamma 2 (IgG2) SAP1.3 F(ab')2 - kappa light chain C214S dimer, 48 kDa Homo sapiens protein
|
Buffer: |
50 mM HEPES, 150 mM KCl, pH: 7.5
|
Experiment: |
SAXS
data collected at BM29, ESRF on 2022 Jun 8
|
Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.
Nat Commun 16(1):3495 (2025)
Elliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I
|
RgGuinier |
3.9 |
nm |
Dmax |
13.7 |
nm |
VolumePorod |
135 |
nm3 |
|
|
|
|
|
Sample: |
Human immunoglobulin gamma 2 (IgG2) SAP1.3 F(ab')2 - heavy chain C226S/C227S dimer, 52 kDa Homo sapiens protein
Human immunoglobulin gamma 2 (IgG2) SAP1.3 F(ab')2 - kappa light chain dimer, 48 kDa Homo sapiens protein
|
Buffer: |
50 mM HEPES, 150 mM KCl, pH: 7.5
|
Experiment: |
SAXS
data collected at BM29, ESRF on 2022 Jun 8
|
Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.
Nat Commun 16(1):3495 (2025)
Elliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I
|
RgGuinier |
4.2 |
nm |
Dmax |
15.1 |
nm |
VolumePorod |
140 |
nm3 |
|
|
|
|
|
Sample: |
Human immunoglobulin gamma 2 (IgG2) SAP1.3 F(ab')2 - kappa light chain dimer, 48 kDa Homo sapiens protein
Human immunoglobulin gamma 2 (IgG2) SAP1.3 F(ab')2 - heavy chain dimer, 52 kDa Homo sapiens protein
|
Buffer: |
50 mM HEPES, 150 mM KCl, pH: 7.5
|
Experiment: |
SAXS
data collected at BM29, ESRF on 2022 Jun 8
|
Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.
Nat Commun 16(1):3495 (2025)
Elliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I
|
RgGuinier |
3.8 |
nm |
Dmax |
12.8 |
nm |
VolumePorod |
126 |
nm3 |
|
|
|
|
|
Sample: |
Human immunoglobulin gamma 1 (IgG1) SAP1.3 F(ab')2 - heavy chain dimer, 51 kDa Homo sapiens protein
Human immunoglobulin gamma 1 (IgG1) SAP1.3 F(ab')2 - kappa light chain dimer, 48 kDa Homo sapiens protein
|
Buffer: |
50 mM HEPES, 150 mM KCl, pH: 7.5
|
Experiment: |
SAXS
data collected at BM29, ESRF on 2022 Jun 8
|
Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.
Nat Commun 16(1):3495 (2025)
Elliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I
|
RgGuinier |
4.5 |
nm |
Dmax |
16.3 |
nm |
VolumePorod |
139 |
nm3 |
|
|
|
|
|
Sample: |
Human immunoglobulin gamma 1 (IgG1) ChiLob 7/4 F(ab')2 - heavy chain dimer, 51 kDa Homo sapiens protein
Human immunoglobulin gamma 1 (IgG1) ChiLob 7/4 F(ab')2 - kappa light chain dimer, 47 kDa Homo sapiens protein
|
Buffer: |
50 mM HEPES, 150 mM KCl, pH: 7.5
|
Experiment: |
SAXS
data collected at BM29, ESRF on 2022 Apr 12
|
Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.
Nat Commun 16(1):3495 (2025)
Elliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I
|
RgGuinier |
4.6 |
nm |
Dmax |
17.4 |
nm |
VolumePorod |
133 |
nm3 |
|
|
|
|
|
Sample: |
Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab')2 - heavy chain dimer, 52 kDa Homo sapiens protein
Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab')2 - kappa light chain dimer, 47 kDa Homo sapiens protein
|
Buffer: |
50 mM HEPES, 150 mM KCl, pH: 7.5
|
Experiment: |
SAXS
data collected at BM29, ESRF on 2022 Jun 8
|
Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.
Nat Commun 16(1):3495 (2025)
Elliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I
|
RgGuinier |
3.9 |
nm |
Dmax |
13.7 |
nm |
VolumePorod |
123 |
nm3 |
|
|