Small Angle Scattering Biological Data Bank SASBDB

SASDNC6 – Ox40 RNA (untranslated region, UTR), alternative decay element (ADEshort)

Ox40 3 UTR ADEshort experimental SAS data

Sample:	Ox40 3 UTR ADEshort monomer, 7 kDa Mus musculus RNA
Buffer:	50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7

NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
Tants JN, Becker LM, McNicoll F, Müller-McNicoll M, Schlundt A

R_g^Guinier	1.7	nm
D_max	9.0	nm
Volume^Porod	12	nm³

SASDM36 – c-Myc12 DnaseI Treated G-quadruplex

c-Myc 12-tract G-quadruplex monomer Dnase I treated experimental SAS data

Sample:	c-Myc 12-tract G-quadruplex monomer Dnase I treated monomer, 22 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Sep 29

Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO

R_g^Guinier	2.0	nm
D_max	6.7	nm
Volume^Porod	25	nm³

SASDM46 – c-Kit12 DNase I Digest

c-Kit 12-tract G-quadruplex monomer Dnase I treated experimental SAS data

c-Kit 12-tract G-quadruplex monomer Dnase I treated Kratky plot

Sample:	c-Kit 12-tract G-quadruplex monomer Dnase I treated monomer, 20 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Sep 29

R_g^Guinier	1.6	nm
D_max	5.7	nm
Volume^Porod	16	nm³

SASDM56 – 2KZD

TERT promoter G-quadruplex antiparallel experimental SAS data

Sample:	TERT promoter G-quadruplex antiparallel monomer, 6 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 13

R_g^Guinier	1.2	nm
D_max	3.6	nm
Volume^Porod	7	nm³

SASDM66 – 2KZE

TERT promoter G-quadruplex parallel experimental SAS data

Sample:	TERT promoter G-quadruplex parallel monomer, 6 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Aug 11

R_g^Guinier	1.3	nm
D_max	4.2	nm
Volume^Porod	8	nm³

SASDM76 – 201D

DNA oligonucleotide G4(T4G4)3 experimental SAS data

Sample:	DNA oligonucleotide G4(T4G4)3 monomer, 9 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 12

R_g^Guinier	1.3	nm
D_max	4.1	nm
Volume^Porod	10	nm³

SASDM86 – 6GH0

cKIT 2-tetrad promoter GQ antiparallel experimental SAS data

Sample:	cKIT 2-tetrad promoter GQ antiparallel monomer, 7 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 21

R_g^Guinier	1.0	nm
D_max	3.1	nm
Volume^Porod	8	nm³

SASDM96 – 2GKU

human telomere 24mer hybrid-1 experimental SAS data

Sample:	human telomere 24mer hybrid-1 monomer, 8 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 11

R_g^Guinier	1.2	nm
D_max	3.8	nm
Volume^Porod	8	nm³

SASDMA6 – 5I2V

kRas promoter GQ hybrid experimental SAS data

Sample:	kRas promoter GQ hybrid monomer, 7 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20

R_g^Guinier	1.3	nm
D_max	4.9	nm
Volume^Porod	10	nm³

SASDMB6 – 6L92

WNT promoter GQ hybrid form experimental SAS data

Sample:	WNT promoter GQ hybrid form monomer, 7 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 21

R_g^Guinier	1.2	nm
D_max	3.9	nm
Volume^Porod	8	nm³

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