Small Angle Scattering Biological Data Bank SASBDB

SASDMC6 – 2KQG

cKit promoter GQ parallel experimental SAS data

Sample:	cKit promoter GQ parallel monomer, 7 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20

Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO

R_g^Guinier	1.6	nm
D_max	3.2	nm
Volume^Porod	14	nm³

SASDMD6 – 2LBY

cMyc promoter GQ parallel experimental SAS data

Sample:	cMyc promoter GQ parallel monomer, 6 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20

R_g^Guinier	1.2	nm
D_max	3.9	nm
Volume^Porod	7	nm³

SASDME6 – 6NEB

cMyc promoter GQ 1:6:1 loop parallel experimental SAS data

Sample:	cMyc promoter GQ 1:6:1 loop parallel monomer, 9 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20

R_g^Guinier	1.4	nm
D_max	4.5	nm
Volume^Porod	10	nm³

SASDMF6 – 1XAV

cMyc promoter modified GQ parallel experimental SAS data

Sample:	cMyc promoter modified GQ parallel monomer, 7 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 11

R_g^Guinier	1.3	nm
D_max	4.1	nm
Volume^Porod	9	nm³

SASDMG6 – 5CMX

two-tetrad antiparallel GQ with duplex junction experimental SAS data

Sample:	two-tetrad antiparallel GQ with duplex junction monomer, 10 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 3

R_g^Guinier	1.5	nm
D_max	5.2	nm
Volume^Porod	12	nm³

SASDMH6 – 2M27

VEGF promoter GQ parallel experimental SAS data

Sample:	VEGF promoter GQ parallel monomer, 7 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20

R_g^Guinier	1.3	nm
D_max	3.8	nm
Volume^Porod	8	nm³

SASDMJ6 – cMyc-8

cMyc promoter 8-tract G-quadruplex experimental SAS data

Sample:	cMyc promoter 8-tract G-quadruplex monomer, 11 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 19

R_g^Guinier	1.4	nm
D_max	4.6	nm
Volume^Porod	13	nm³

SASDMK6 – cKit-8

cKit promoter 8-tract G-quadruplex experimental SAS data

Sample:	cKit promoter 8-tract G-quadruplex monomer, 12 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 19

R_g^Guinier	1.5	nm
D_max	4.7	nm
Volume^Porod	15	nm³

SASDML6 – kRas-8

kRas promoter 8-tract G-quadruplex experimental SAS data

Sample:	kRas promoter 8-tract G-quadruplex monomer, 14 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 19

R_g^Guinier	1.7	nm
D_max	5.6	nm
Volume^Porod	16	nm³

SASDMM6 – cMyc-12

cMyc promoter 12-tract G-quadruplex experimental SAS data

cMyc promoter 12-tract G-quadruplex Kratky plot

Sample:	cMyc promoter 12-tract G-quadruplex monomer, 23 kDa synthetic construct DNA
Buffer:	6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 22

R_g^Guinier	2.3	nm
D_max	8.4	nm
Volume^Porod	29	nm³

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