|
|
|
Sample: |
Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
|
Buffer: |
25 mM Tris 100 mM NaCl, pH: 7.5
|
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2020 May 1
|
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2
Nature Communications 11(1) (2020)
Custódio T, Das H, Sheward D, Hanke L, Pazicky S, Pieprzyk J, Sorgenfrei M, Schroer M, Gruzinov A, Jeffries C, Graewert M, Svergun D, Dobrev N, Remans K, Seeger M, McInerney G, Murrell B, Hällberg B, ...
|
RgGuinier |
3.0 |
nm |
Dmax |
13.1 |
nm |
VolumePorod |
64 |
nm3 |
|
|
|
|
|
Sample: |
Synthetic nanobody Sybody 23 monomer, 16 kDa synthetic construct protein
Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
|
Buffer: |
25 mM Tris 100 mM NaCl, pH: 7.5
|
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2020 May 10
|
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2
Nature Communications 11(1) (2020)
Custódio T, Das H, Sheward D, Hanke L, Pazicky S, Pieprzyk J, Sorgenfrei M, Schroer M, Gruzinov A, Jeffries C, Graewert M, Svergun D, Dobrev N, Remans K, Seeger M, McInerney G, Murrell B, Hällberg B, ...
|
RgGuinier |
3.5 |
nm |
Dmax |
15.1 |
nm |
VolumePorod |
87 |
nm3 |
|
|
|
|
|
Sample: |
Candidatus Glomeribacter gigasporarum cyclodipeptide synthase monomer, 34 kDa Candidatus Glomeribacter gigasporarum protein
E. coli Phe-tRNAPhe monomer, 25 kDa Escherichia coli RNA
|
Buffer: |
10 mM MOPS pH6.7; 200 mM NaCl, 8 mM MgCl2, pH: 6.7
|
Experiment: |
SAXS
data collected at SWING, SOLEIL on 2016 Oct 2
|
Structural basis of the interaction between cyclodipeptide synthases and aminoacylated tRNA substrates.
RNA 26(11):1589-1602 (2020)
Bourgeois G, Seguin J, Babin M, Gondry M, Mechulam Y, Schmitt E
|
RgGuinier |
3.3 |
nm |
Dmax |
14.0 |
nm |
VolumePorod |
77 |
nm3 |
|
|
|
|
|
Sample: |
primary microRNA pri-miR16-1 monomer, 36 kDa Homo sapiens RNA
|
Buffer: |
50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, pH: 7.5
|
Experiment: |
SAXS
data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12
|
Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures
Biophysical Journal (2020)
Pabit S, Chen Y, Usher E, Cook E, Pollack L, Showalter S
|
RgGuinier |
4.4 |
nm |
Dmax |
17.2 |
nm |
VolumePorod |
74 |
nm3 |
|
|
|
|
|
Sample: |
primary microRNA pri-miR16-1 complexed with DGCR8-core protein monomer, 36 kDa Homo sapiens RNA
Microprocessor complex subunit DGCR8 monomer, 26 kDa Homo sapiens protein
|
Buffer: |
50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, 50% sucrose, DGCR8-core, pH: 7.5
|
Experiment: |
SAXS
data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12
|
Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures
Biophysical Journal (2020)
Pabit S, Chen Y, Usher E, Cook E, Pollack L, Showalter S
|
RgGuinier |
5.0 |
nm |
Dmax |
17.2 |
nm |
VolumePorod |
125 |
nm3 |
|
|
|
|
|
Sample: |
Apoferritin light chain 24-mer, 479 kDa Equus caballus protein
|
Buffer: |
50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
|
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2019 Apr 5
|
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data
Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
|
RgGuinier |
5.4 |
nm |
Dmax |
12.5 |
nm |
VolumePorod |
679 |
nm3 |
|
|
|
|
|
Sample: |
Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
|
Buffer: |
50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
|
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2019 Apr 5
|
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data
Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
|
RgGuinier |
1.8 |
nm |
Dmax |
5.1 |
nm |
VolumePorod |
37 |
nm3 |
|
|
|
|
|
Sample: |
Bovine serum albumin monomer, 66 kDa Bos taurus protein
|
Buffer: |
50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
|
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2019 Apr 5
|
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data
Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
|
RgGuinier |
2.8 |
nm |
Dmax |
8.0 |
nm |
VolumePorod |
98 |
nm3 |
|
|
|
|
|
Sample: |
Bovine serum albumin dimer, 133 kDa Bos taurus protein
|
Buffer: |
50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
|
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2019 Apr 5
|
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data
Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
|
RgGuinier |
4.0 |
nm |
Dmax |
13.2 |
nm |
VolumePorod |
211 |
nm3 |
|
|
|
|
|
Sample: |
Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae protein
|
Buffer: |
50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
|
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2019 Apr 5
|
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data
Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
|
RgGuinier |
3.3 |
nm |
Dmax |
9.3 |
nm |
VolumePorod |
201 |
nm3 |
|
|