Small Angle Scattering Biological Data Bank SASBDB

SASDAD4 – Full length GtBP3

Sample:	full length CtBP3 tetramer, 170 kDa protein
Buffer:	25 mM Tris/HCl 250 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III on 2004 Nov 18

The C-terminal domain of the transcriptional corepressor CtBP is intrinsically unstructured. Protein Sci 15(5):1042-50 (2006)
Nardini M, Svergun D, Konarev PV, Spanò S, Fasano M, Bracco C, Pesce A, Donadini A, Cericola C, Secundo F, Luini A, Corda D, Bolognesi M

R_g^Guinier	5.1	nm
D_max	19.0	nm
Volume^Porod	330	nm³

SASDAE4 – C-terminal CtBP3

Sample:	C-term part CtBP3 dimer, 52 kDa protein
Buffer:	25 mM Tris/HCl 250 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III on 2004 Nov 18

R_g^Guinier	5.5	nm
D_max	20.0	nm
Volume^Porod	126	nm³

SASDC52 – Bruton tyrosine kinase

Tyrosine-protein kinase BTK (R28C mutant) experimental SAS data

Sample:	Tyrosine-protein kinase BTK (R28C mutant) monomer, 76 kDa Homo sapiens protein
Buffer:	20 mM HEPES 200 mM NaCl, 2 mM DTT and 1 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III on 2002 Apr 2

Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering. EMBO J 22(18):4616-24 (2003)
Márquez JA, Smith CI, Petoukhov MV, Lo Surdo P, Mattsson PT, Knekt M, Westlund A, Scheffzek K, Saraste M, Svergun DI

R_g^Guinier	5.0	nm
D_max	20.0	nm
Volume^Porod	130	nm³

SASDAX2 – Pyruvate decarboxylase (PDC) from Z. mobilis

Pyruvate decarboxylase experimental SAS data

Sample:	Pyruvate decarboxylase tetramer, 244 kDa Zymomonas mobilis protein
Buffer:	100 mM Sodium Citrate, 17% Glycerol, 22.5% PEG 1500, pH: 6
Experiment:	SAXS data collected at EMBL X33, DORIS III on 1998 Nov 3

Crystal versus solution structures of thiamine diphosphate-dependent enzymes. J Biol Chem 275(1):297-302 (2000)
Svergun DI, Petoukhov MV, Koch MH, König S

R_g^Guinier	3.9	nm
D_max	11.0	nm

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