Small Angle Scattering Biological Data Bank SASBDB

SASDJU9 – Mammalian cell entry protein 1A (Mce1A36-148)

Mce-family protein Mce1A monomer experimental SAS data

Sample:	Mce-family protein Mce1A monomer monomer, 17 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris, 350 mM NaCl, 10% Glycerol, pH: 8.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 May 10

Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis IUCrJ 8(5) (2021)
Asthana P, Singh D, Pedersen J, Hynönen M, Sulu R, Murthy A, Laitaoja M, Jänis J, Riley L, Venkatesan R

R_g^Guinier	2.1	nm
D_max	8.7	nm
Volume^Porod	29	nm³

SASDJV9 – Mammalian cell entry protein 4A (Mce4A39-140)

Mce-family protein Mce4A experimental SAS data

Sample:	Mce-family protein Mce4A monomer, 15 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50mM MOPS, 350mM NaCl, 10% Glycerol, pH: 7
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 May 10

R_g^Guinier	2.1	nm
D_max	7.8	nm
Volume^Porod	22	nm³

SASDJW9 – Mammalian cell entry protein 4A (Mce4A36-400)

Mce-family protein Mce4An-Dodecyl-β-D-Maltopyranoside experimental SAS data

Sample:	Mce-family protein Mce4A monomer, 44 kDa Mycobacterium tuberculosis (strain … protein n-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
Buffer:	50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 13

R_g^Guinier	5.7	nm
D_max	21.5	nm
Volume^Porod	446	nm³

SASDJX9 – Mammalian cell entry protein 4A (Mce4A121-400)

n-Dodecyl-β-D-MaltopyranosideMce-family protein Mce4A experimental SAS data

Sample:	n-Dodecyl-β-D-Maltopyranoside 0, 102 kDa Mce-family protein Mce4A monomer, 35 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Nov 28

R_g^Guinier	5.0	nm
D_max	19.1	nm
Volume^Porod	278	nm³

SASDJY9 – Mammalian cell entry protein 4A (Mce4A39-320)

Sample:	n-Dodecyl-β-D-Maltopyranoside 0, 102 kDa Mce-family protein Mce4A monomer, 34 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 May 2

R_g^Guinier	4.8	nm
D_max	18.2	nm
Volume^Porod	189	nm³

SASDJZ9 – Mammalian cell entry protein 1A (Mce1A38-325)

Mce-family protein Mce1An-Dodecyl-β-D-Maltopyranoside experimental SAS data

Sample:	Mce-family protein Mce1A monomer, 36 kDa Mycobacterium tuberculosis protein n-Dodecyl-β-D-Maltopyranoside 0, 82 kDa
Buffer:	50 mM Tris, 500 mM NaCl, 10% Glycerol, 5 mM DDM, 1 mM β-ME, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 May 2

R_g^Guinier	4.6	nm
D_max	17.5	nm
Volume^Porod	151	nm³

SASDK22 – Mammalian cell entry protein 1A (Mce1A126-454)

Sample:	Mce-family protein Mce1A monomer, 39 kDa Mycobacterium tuberculosis protein n-Dodecyl-β-D-Maltopyranoside 0, 123 kDa
Buffer:	50 mM Tris, 350 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Nov 28

R_g^Guinier	5.5	nm
D_max	22.0	nm
Volume^Porod	216	nm³

SASDK32 – Mammalian cell entry protein 1A (Mce1A38-454)

Sample:	Mce-family protein Mce1A monomer, 48 kDa Mycobacterium tuberculosis protein n-Dodecyl-β-D-Maltopyranoside 0, 112 kDa
Buffer:	50 mM Tris, 500 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 13

R_g^Guinier	5.3	nm
D_max	21.6	nm
Volume^Porod	362	nm³

SASDKJ4 – SARS-CoV-2 nsp7 and nsp8

Replicase polyprotein 1a - nsp7Replicase polyprotein 1a - nsp8 experimental SAS data

Sample:	Replicase polyprotein 1a - nsp7 , 18 kDa Severe acute respiratory … protein Replicase polyprotein 1a - nsp8 , 44 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, pH 8.0, 100 mM NaCl, 4 mM DTT, 4 mM MgCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 19

Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes Science Advances 7(10):eabf1004 (2021)
Krichel B, Bylapudi G, Schmidt C, Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C

R_g^Guinier	3.4	nm
D_max	13.5	nm
Volume^Porod	92	nm³

SASDFZ7 – Full-length myotilin with N-terminally fused thioredoxin tag

Full-length myotilin with N-terminally fused thioredoxin tag Rg histogram

Sample:	his-trx-myotilin monomer, 70 kDa Homo sapiens protein
Buffer:	20 mM Tris, 400 mM NaCl, 250 mM arginine, 5% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jun 11

Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L

R_g^Guinier	5.1	nm
D_max	16.7	nm
Volume^Porod	131	nm³

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