SASDH29 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM phosphate, pH 6.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM phosphate, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.7 nm
VolumePorod 240 nm3

SASDHG4 – NopAA, a type three effector from Sinorhizobium fredii USDA257 whith xyloglucanase activity

Type III effector NopAA experimental SAS data
ALLOSMOD model
Sample: Type III effector NopAA monomer, 31 kDa Sinorhizobium fredii USDA257 protein
Buffer: PBS, 150 mM NaCl, 10% glycerol, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 17
Structural and enzymatic characterisation of the Type III effector NopAA (=GunA) from Sinorhizobium fredii USDA257 reveals a Xyloglucan hydrolase activity. Sci Rep 10(1):9932 (2020)
Dorival J, Philys S, Giuntini E, Brailly R, de Ruyck J, Czjzek M, Biondi E, Bompard C
RgGuinier 2.4 nm
Dmax 9.9 nm
VolumePorod 38 nm3

SASDH55 – Modified RNA stem loop IV poliovirus IRES (SLIVm)

modified stem loop IV poliovirus IRES, nucleotides 278-398 experimental SAS data
modified stem loop IV poliovirus IRES, nucleotides 278-398 Kratky plot
Sample: modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Dec 2
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 3.5 nm
Dmax 12.0 nm
VolumePorod 94 nm3

SASDH65 – Poly(rC)-binding protein 2 (PCBP2)

Poly(rC)-binding protein 2 experimental SAS data
Poly(rC)-binding protein 2 Kratky plot
Sample: Poly(rC)-binding protein 2 monomer, 40 kDa Homo sapiens protein
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jul 16
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 3.3 nm
Dmax 13.2 nm
VolumePorod 76 nm3

SASDH75 – Poly(rC)-binding protein 2 (PCBP2) with bound modified RNA stem loop IV poliovirus IRES (SLIVm) - PCBP2/SLIVm complex

Poly(rC)-binding protein 2modified stem loop IV poliovirus IRES, nucleotides 278-398 experimental SAS data
Poly(rC)-binding protein 2 modified stem loop IV poliovirus IRES, nucleotides 278-398 Kratky plot
Sample: Poly(rC)-binding protein 2 monomer, 40 kDa Homo sapiens protein
modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Dec 16
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 3.7 nm
Dmax 11.5 nm
VolumePorod 162 nm3

SASDH85 – Truncated poly(rC)-binding protein 2 (PCBP2-ΔKH3) with bound modified RNA stem loop IV poliovirus IRES (SLIVm)

modified stem loop IV poliovirus IRES, nucleotides 278-398Truncated poly(rC)-binding protein 2 (ΔKH3)Truncated poly(rC)-binding protein 2 (ΔKH3) experimental SAS data
modified stem loop IV poliovirus IRES, nucleotides 278-398 Truncated poly(rC)-binding protein 2 (ΔKH3) Truncated poly(rC)-binding protein 2 (ΔKH3) Kratky plot
Sample: modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA
Truncated poly(rC)-binding protein 2 (ΔKH3) monomer, 28 kDa Homo sapiens protein
Truncated poly(rC)-binding protein 2 (ΔKH3) monomer, 28 kDa Homo sapiens protein
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jul 1
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 3.8 nm
Dmax 12.2 nm
VolumePorod 165 nm3

SASDH95 – Truncated Poly(rC)-binding protein 2 (PCBP2-ΔKH3)

Truncated poly(rC)-binding protein 2 (ΔKH3) experimental SAS data
Truncated poly(rC)-binding protein 2 (ΔKH3) Kratky plot
Sample: Truncated poly(rC)-binding protein 2 (ΔKH3) monomer, 28 kDa Homo sapiens protein
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jan 1
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 2.6 nm
Dmax 9.2 nm
VolumePorod 66 nm3

SASDHA5 – Truncated poly(rC)-binding protein 2 (PCBP2-ΔKH1-KH2) with bound modified RNA stem loop IV poliovirus IRES (SLIVm)

modified stem loop IV poliovirus IRES, nucleotides 278-398Truncated poly(rC)-binding protein 2 (ΔKH1-KH2) experimental SAS data
modified stem loop IV poliovirus IRES, nucleotides 278-398 Truncated poly(rC)-binding protein 2 (ΔKH1-KH2) Kratky plot
Sample: modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA
Truncated poly(rC)-binding protein 2 (ΔKH1-KH2) monomer, 18 kDa Homo sapiens protein
Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jul 1
Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
RgGuinier 3.5 nm
Dmax 12.2 nm
VolumePorod 126 nm3

SASDHW2 – The Legionella pneumophila type II secretion system substrate NttE

Uncharacterized protein experimental SAS data
GASBOR model
Sample: Uncharacterized protein dimer, 66 kDa Legionella pneumophila protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 18
Structure, Dynamics and Cellular Insight Into Novel Substrates of the Legionella pneumophila Type II Secretion System Frontiers in Molecular Biosciences 7 (2020)
Portlock T, Tyson J, Dantu S, Rehman S, White R, McIntire I, Sewell L, Richardson K, Shaw R, Pandini A, Cianciotto N, Garnett J
RgGuinier 2.9 nm
Dmax 9.4 nm
VolumePorod 114 nm3

SASDFN6 – DNA-binding protein HU-alpha

DNA-binding protein HU-alpha experimental SAS data
CHIMERA model
Sample: DNA-binding protein HU-alpha octamer, 77 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 May 27
Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 3.2 nm
Dmax 10.7 nm

4780 hits found.