Small Angle Scattering Biological Data Bank SASBDB

SASDJJ6 – Sarcomeric intrinsically disordered protein FATZ-1 (N-FATZ-1)

N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data

Sarcomeric intrinsically disordered protein FATZ-1 (N-FATZ-1) Rg histogram

Sample:	N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 20 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 29

Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin. Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto ...

R_g^Guinier	3.5	nm
D_max	14.1	nm
Volume^Porod	46	nm³

SASDJK6 – Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1)

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data

Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1) Rg histogram

Sample:	Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2017 Jul 18

R_g^Guinier	3.9	nm
D_max	17.3	nm
Volume^Porod	66	nm³

SASDJL6 – Sarcomeric F-actin crosslinking protein α-actinin-2 (spectrin repeat rod domain, rod-α-actinin-2)

Rod domain of α-actinin-2 experimental SAS data

Sample:	Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Dec 5

R_g^Guinier	6.7	nm
D_max	27.2	nm
Volume^Porod	214	nm³

SASDJM6 – Sarcomeric F-actin crosslinking protein α-actinin-2 (half-dimer, hd)

Half dimer of α-actinin-2 experimental SAS data

Sarcomeric F-actin crosslinking protein α-actinin-2 (half-dimer, hd) Rg histogram

Sample:	Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 18

R_g^Guinier	5.3	nm
D_max	22.0	nm
Volume^Porod	172	nm³

SASDJN6 – Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1)

Rod domain of α-actinin-2Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data

Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1) Rg histogram

Sample:	Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) dimer, 43 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 18

R_g^Guinier	7.6	nm
D_max	28.4	nm
Volume^Porod	371	nm³

SASDJP6 – Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (hd-α-actinin-2/Δ91-FATZ-1)

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2)Half dimer of α-actinin-2 experimental SAS data

Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (hd-α-actinin-2/Δ91-FATZ-1) Rg histogram

Sample:	Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 18

R_g^Guinier	5.8	nm
D_max	22.5	nm
Volume^Porod	242	nm³

SASDJ85 – Zinc transporter 1 – hZnT1-CTD

Zinc transporter 1 experimental SAS data

Sample:	Zinc transporter 1 dimer, 37 kDa Homo sapiens protein
Buffer:	20mM Tris, 150mM NaCl, 1mM TCEP, pH: 7.6
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 14

Heterologous Expression and Biochemical Characterization of the Human Zinc Transporter 1 (ZnT1) and Its Soluble C-Terminal Domain Frontiers in Chemistry 9 (2021)
Cotrim C, Jarrott R, Whitten A, Choudhury H, Drew D, Martin J

R_g^Guinier	2.7	nm
D_max	10.0	nm
Volume^Porod	50	nm³

SASDKQ9 – Chaperone protein IpgC, symmetric dimer from Shigella flexneri

Chaperone protein IpgC experimental SAS data

Sample:	Chaperone protein IpgC dimer, 39 kDa Shigella flexneri protein
Buffer:	20 mM HEPES, 100 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2012 Dec 10

Structural Insights of Shigella Translocator IpaB and Its Chaperone IpgC in Solution Frontiers in Cellular and Infection Microbiology 11 (2021)
Ferrari M, Charova S, Sansonetti P, Mylonas E, Gazi A

R_g^Guinier	2.7	nm
D_max	9.0	nm
Volume^Porod	56	nm³

SASDKR9 – The chaperone protein IpgC/invasin IpaB heterodimer complex from Shigella flexneri

T3SS Chaperone protein IpgCInvasin IpaB experimental SAS data

Sample:	T3SS Chaperone protein IpgC monomer, 20 kDa Shigella flexneri protein Invasin IpaB monomer, 62 kDa Shigella flexneri protein
Buffer:	20 mM HEPES, 100 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2012 Dec 10

R_g^Guinier	3.9	nm
D_max	14.0	nm
Volume^Porod	165	nm³

SASDKA2 – Two titin I27 domains linked with RS linker

Two titin I27 domains linked with RS linker experimental SAS data

Two titin I27 domains linked with RS linker Rg histogram

Sample:	Two titin I27 domains linked with RS linker monomer, 23 kDa protein
Buffer:	50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S

R_g^Guinier

3.0

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