Small Angle Scattering Biological Data Bank SASBDB

SASDLR2 – PfMDH L-lactate dehydrogenase bound to inhibitor 2a

L-lactate dehydrogenase experimental SAS data

Sample:	L-lactate dehydrogenase tetramer, 141 kDa Plasmodium falciparum protein
Buffer:	100 mM Na-phosphate buffer, 400 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Xenocs Xeuss 2.0 with MetalJet, Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University on 2019 Jul 3

A fragment-based approach identifies an allosteric pocket that impacts malate dehydrogenase activity Communications Biology 4(1) (2021)
Reyes Romero A, Lunev S, Popowicz G, Calderone V, Gentili M, Sattler M, Plewka J, Taube M, Kozak M, Holak T, Dömling A, Groves M

R_g^Guinier	3.4	nm
D_max	10.5	nm
Volume^Porod	244	nm³

SASDLS2 – PfMDH L-lactate dehydrogenase bound to inhibitor 6a

Sample:	L-lactate dehydrogenase tetramer, 141 kDa Plasmodium falciparum protein
Buffer:	100 mM Na-phosphate buffer, 400 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Xenocs Xeuss 2.0 with MetalJet, Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University on 2019 Jul 3

R_g^Guinier	3.6	nm
D_max	11.4	nm
Volume^Porod	223	nm³

SASDJ38 – Monomeric Jagged1 high-affinity full ectodomain

Sample:	Protein jagged-1 monomer, 115 kDa Mus musculus protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Dec 12

Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC

R_g^Guinier	7.4	nm
D_max	24.0	nm
Volume^Porod	375	nm³

SASDJ48 – Jagged1 high-affinity full ectodomain 5.3 µM

Sample:	Protein jagged-1 , 115 kDa Mus musculus protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Dec 11

R_g^Guinier	8.1	nm
D_max	30.0	nm
Volume^Porod	430	nm³

SASDJ58 – Jagged1 high-affinity full ectodomain 11 µM

Sample:	Protein jagged-1 , 115 kDa Mus musculus protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Dec 11

R_g^Guinier	8.9	nm
D_max	33.0	nm
Volume^Porod	505	nm³

SASDJ68 – Jagged1 high-affinity full ectodomain 21 µM

Sample:	Protein jagged-1 , 115 kDa Mus musculus protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Dec 11

R_g^Guinier	9.6	nm
D_max	42.0	nm
Volume^Porod	715	nm³

SASDJ78 – Jagged1 high-affinity full ectodomain 42 µM

Sample:	Protein jagged-1 , 115 kDa Mus musculus protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Dec 11

R_g^Guinier	10.2	nm
D_max	43.0	nm
Volume^Porod	900	nm³

SASDJ88 – Jagged1 EGF8-11 29 µM

Protein jagged-1 EGF8-11 experimental SAS data

Sample:	Protein jagged-1 EGF8-11 , 21 kDa Mus musculus protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Dec 11

R_g^Guinier	3.3	nm
D_max	11.0	nm
Volume^Porod	26	nm³

SASDJ98 – Jagged1 EGF8-11 58 µM

Sample:	Protein jagged-1 EGF8-11 , 21 kDa Mus musculus protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Dec 11

R_g^Guinier	3.2	nm
D_max	11.5	nm
Volume^Porod	31	nm³

SASDJA8 – Jagged1 EGF8-11 115 µM

Sample:	Protein jagged-1 EGF8-11 , 21 kDa Mus musculus protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Dec 11

R_g^Guinier	3.1	nm
D_max	11.5	nm
Volume^Porod	34	nm³

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