SASDHN5 – Apolipoprotein-D plus sphingomyelin

Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 9.9 nm
VolumePorod 171 nm3

SASDJ46 – Fep1-rec-GATA

GATA-type iron responsive transcription factor Fep1 reconstituted24-mer double strand DNA from the GATA promoter experimental SAS data
GATA-type iron responsive transcription factor Fep1 reconstituted 24-mer double strand DNA from the GATA promoter Kratky plot
Sample: GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein
24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.5 nm
Dmax 13.1 nm
VolumePorod 73 nm3

SASDJ66 – GATA double strand DNA

24-mer double strand DNA from the GATA promoter experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 10 nm3

SASDJ86 – Fep1 wild type

GATA-type iron responsive transcription factor Fep1 experimental SAS data
Fep1 wild type Rg histogram
Sample: GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.5 nm
Dmax 11.8 nm
VolumePorod 41 nm3

SASDJC5 – LINC complex between the SUN domain of SUN1 and KASH domain of Nesprin-4 - SUN1-KASH4 6:6 complex

SUN domain-containing protein 1Nesprin-4 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: SUN domain-containing protein 1 hexamer, 135 kDa Homo sapiens protein
Nesprin-4 hexamer, 20 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 21
A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies. Elife 10 (2021)
Gurusaran M, Davies OR
RgGuinier 4.0 nm
Dmax 13.5 nm
VolumePorod 240 nm3

SASDJD5 – LINC complex between the SUN domain of SUN1 and KASH domain of KASH5 - SUN1-KASH5 6:6 complex

SUN domain-containing protein 1Protein KASH5 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: SUN domain-containing protein 1 hexamer, 135 kDa Homo sapiens protein
Protein KASH5 hexamer, 20 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Dec 17
A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies. Elife 10 (2021)
Gurusaran M, Davies OR
RgGuinier 3.8 nm
Dmax 13.5 nm
VolumePorod 244 nm3

SASDJE5 – LINC complex between the SUN domain of SUN1 and KASH domain of Nesprin-1 - SUN1-KASH1 6:6 complex

SUN domain-containing protein 1Nesprin-1 experimental SAS data
CORAL model
Sample: SUN domain-containing protein 1 hexamer, 135 kDa Homo sapiens protein
Nesprin-1 hexamer, 22 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Dec 17
A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies. Elife 10 (2021)
Gurusaran M, Davies OR
RgGuinier 3.9 nm
Dmax 13.0 nm
VolumePorod 254 nm3

SASDJF5 – SUN domain of SUN1 harbouring mutation I673E - monomer

SUN domain-containing protein 1, I673E experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: SUN domain-containing protein 1, I673E monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jul 29
A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies. Elife 10 (2021)
Gurusaran M, Davies OR
RgGuinier 2.1 nm
Dmax 8.2 nm
VolumePorod 42 nm3

SASDJ56 – Fep1-GATA

GATA-type iron responsive transcription factor Fep124-mer double strand DNA from the GATA promoter experimental SAS data
GATA-type iron responsive transcription factor Fep1 24-mer double strand DNA from the GATA promoter Kratky plot
Sample: GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein
24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 85 nm3

SASDJ76 – Fep1 wild type with [2Fe2S] cluster reconstituted

GATA-type iron responsive transcription factor Fep1 reconstituted experimental SAS data
Fep1 wild type with [2Fe2S] cluster reconstituted Rg histogram
Sample: GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.8 nm
Dmax 16.5 nm
VolumePorod 53 nm3

5170 hits found.