SASDFB6 – The periplasmically localised protease PqqL from Escherichia coli

Zinc protease PqqL experimental SAS data
DAMMIF model
Sample: Zinc protease PqqL monomer, 102 kDa Escherichia coli protein
Buffer: 20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jun 14
Protease-associated import systems are widespread in Gram-negative bacteria. PLoS Genet 15(10):e1008435 (2019)
Grinter R, Leung PM, Wijeyewickrema LC, Littler D, Beckham S, Pike RN, Walker D, Greening C, Lithgow T
RgGuinier 4.0 nm
Dmax 13.7 nm
VolumePorod 207 nm3

SASDG26 – Wild type 4-hydroxy-tetrahydrodipicolinate synthase

4-hydroxy-tetrahydrodipicolinate synthase experimental SAS data
DAMFILT model
Sample: 4-hydroxy-tetrahydrodipicolinate synthase tetramer, 131 kDa Campylobacter jejuni protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Aug 2
Asparagine-84, a regulatory allosteric site residue, helps maintain the quaternary structure of Campylobacter jejuni dihydrodipicolinate synthase. J Struct Biol :107409 (2019)
Majdi Yazdi M, Saran S, Mrozowich T, Lehnert C, Patel TR, Sanders DAR, Palmer DRJ
RgGuinier 3.4 nm
Dmax 9.0 nm
VolumePorod 188 nm3

SASDG36 – 4-hydroxy-tetrahydrodipicolinate synthase (N84D mutant)

4-hydroxy-tetrahydrodipicolinate synthase (N84D mutant) experimental SAS data
DAMFILT model
Sample: 4-hydroxy-tetrahydrodipicolinate synthase (N84D mutant) dimer, 65 kDa Campylobacter jejuni protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Aug 2
Asparagine-84, a regulatory allosteric site residue, helps maintain the quaternary structure of Campylobacter jejuni dihydrodipicolinate synthase. J Struct Biol :107409 (2019)
Majdi Yazdi M, Saran S, Mrozowich T, Lehnert C, Patel TR, Sanders DAR, Palmer DRJ
RgGuinier 3.1 nm
Dmax 9.5 nm
VolumePorod 108 nm3

SASDG46 – 4-hydroxy-tetrahydrodipicolinate synthase (N84A mutant)

4-hydroxy-tetrahydrodipicolinate synthase (N84A mutant) experimental SAS data
DAMFILT model
Sample: 4-hydroxy-tetrahydrodipicolinate synthase (N84A mutant) , 33 kDa Campylobacter jejuni protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Aug 2
Asparagine-84, a regulatory allosteric site residue, helps maintain the quaternary structure of Campylobacter jejuni dihydrodipicolinate synthase. J Struct Biol :107409 (2019)
Majdi Yazdi M, Saran S, Mrozowich T, Lehnert C, Patel TR, Sanders DAR, Palmer DRJ
RgGuinier 3.3 nm
Dmax 8.9 nm
VolumePorod 163 nm3

SASDH25 – Actinobacterial phosphodiesterase targeting cyclic di-AMP (AtaC)

Alkaline phosphodiesterase I or Nucleotide pyrophosphatase experimental SAS data
Alkaline phosphodiesterase I or Nucleotide pyrophosphatase Kratky plot
Sample: Alkaline phosphodiesterase I or Nucleotide pyrophosphatase monomer, 46 kDa Streptomyces venezuelae protein
Buffer: 20 mM HEPES, 200mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 17
c-di-AMP hydrolysis by a novel type of phosphodiesterase promotes differentiation of multicellular bacteria (2019)
Latoscha A, Drexler D, Al-Bassam M, Kaever V, Findlay K, Witte G, Tschowri N
RgGuinier 2.4 nm
Dmax 7.8 nm
VolumePorod 74 nm3

SASDH35 – Soluble cyclic di-AMP binding protein CpeA (vnz_28055), regulator of CpeB

Cyclic di-AMP binding protein (Putative regulatory, ligand-binding protein) experimental SAS data
Cyclic di-AMP binding protein (Putative regulatory, ligand-binding protein) Kratky plot
Sample: Cyclic di-AMP binding protein (Putative regulatory, ligand-binding protein) dimer, 38 kDa Streptomyces venezuelae protein
Buffer: 200 mM NaCl, 30 mM NaPi, 5% (v/v) glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 4
c-di-AMP hydrolysis by a novel type of phosphodiesterase promotes differentiation of multicellular bacteria (2019)
Latoscha A, Drexler D, Al-Bassam M, Kaever V, Findlay K, Witte G, Tschowri N
RgGuinier 2.8 nm
Dmax 9.2 nm
VolumePorod 69 nm3

SASDHA2 – Soluble guanylate cyclase (unbound)

Soluble guanylyl cyclase alpha-1 subunitSoluble guanylyl cyclase beta-1 subunit experimental SAS data
BILBOMD model
Sample: Soluble guanylyl cyclase alpha-1 subunit monomer, 78 kDa Manduca sexta protein
Soluble guanylyl cyclase beta-1 subunit monomer, 68 kDa Manduca sexta protein
Buffer: 50 mM KH2PO4, 150 mM NaCl, 2% glycerol,, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 3
Allosteric activation of the nitric oxide receptor soluble guanylate cyclase mapped by cryo-electron microscopy. Elife 8 (2019)
Horst BG, Yokom AL, Rosenberg DJ, Morris KL, Hammel M, Hurley JH, Marletta MA
RgGuinier 4.3 nm
Dmax 13.3 nm
VolumePorod 230 nm3

SASDHB2 – Soluble guanylate cyclase (nitric oxide, NO, bound)

Soluble guanylyl cyclase alpha-1 subunitSoluble guanylyl cyclase beta-1 subunit experimental SAS data
BILBOMD model
Sample: Soluble guanylyl cyclase alpha-1 subunit monomer, 78 kDa Manduca sexta protein
Soluble guanylyl cyclase beta-1 subunit monomer, 68 kDa Manduca sexta protein
Buffer: 50 mM KH2PO4,150 mM NaCl, 2% glycerol, 500 µM NO,, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 3
Allosteric activation of the nitric oxide receptor soluble guanylate cyclase mapped by cryo-electron microscopy. Elife 8 (2019)
Horst BG, Yokom AL, Rosenberg DJ, Morris KL, Hammel M, Hurley JH, Marletta MA
RgGuinier 4.4 nm
Dmax 14.2 nm
VolumePorod 218 nm3

SASDF58 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 10mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data
DNA-directed RNA polymerase subunit delta Kratky plot
Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zi...
RgGuinier 3.5 nm
Dmax 14.0 nm
VolumePorod 38 nm3

SASDF68 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 200 mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data
DNA-directed RNA polymerase subunit delta Kratky plot
Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zi...
RgGuinier 3.9 nm
Dmax 20.0 nm
VolumePorod 56 nm3

4692 hits found.