Small Angle Scattering Biological Data Bank SASBDB

SASDF78 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 400 mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data

DNA-directed RNA polymerase subunit delta Kratky plot

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zi...

R_g^Guinier	4.2	nm
D_max	20.0	nm
Volume^Porod	63	nm³

SASDF88 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 800 mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.5	nm
D_max	21.0	nm
Volume^Porod	77	nm³

SASDF98 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 10mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data

DNA-directed RNA polymerase subunit delta - mutant Kratky plot

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.3	nm
D_max	19.5	nm
Volume^Porod	58	nm³

SASDFA8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 200mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.6	nm
D_max	22.0	nm
Volume^Porod	76	nm³

SASDFB8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 400mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.5	nm
D_max	24.0	nm
Volume^Porod	78	nm³

SASDFC8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 800mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.5	nm
D_max	21.0	nm
Volume^Porod	74	nm³

SASDGD4 – Mixed lineage leukemia protein-1 complex, MLL1-WDR5-ASH2L-RBBP5(2-381)

Retinoblastoma-binding protein 5Histone-lysine N-methyltransferase 2AWD repeat-containing protein 5Set1/Ash2 histone methyltransferase complex subunit ASH2 experimental SAS data

Sample:	Retinoblastoma-binding protein 5 monomer, 42 kDa Homo sapiens protein Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein WD repeat-containing protein 5 monomer, 34 kDa Homo sapiens protein Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein
Buffer:	300 mM NaCl, 25mM Tris-HCl, 4% glycerol, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Jun 22

The internal interaction in RBBP5 regulates assembly and activity of MLL1 methyltransferase complex. Nucleic Acids Res (2019)
Han J, Li T, Li Y, Li M, Wang X, Peng C, Su C, Li N, Li Y, Xu Y, Chen Y

R_g^Guinier	5.7	nm
D_max	18.6	nm
Volume^Porod	360	nm³

SASDGE4 – Mixed lineage leukemia protein-1 complex, MLL1-WDR5-ASH2L-RBBP5(2-480)

Histone-lysine N-methyltransferase 2AWD repeat-containing protein 5Set1/Ash2 histone methyltransferase complex subunit ASH2Retinoblastoma-binding protein 5 experimental SAS data

Sample:	Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein WD repeat-containing protein 5 monomer, 34 kDa Homo sapiens protein Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein Retinoblastoma-binding protein 5 monomer, 53 kDa Homo sapiens protein
Buffer:	300 mM NaCl, 25mM Tris-HCl, 4% glycerol, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Jun 22

R_g^Guinier	5.0	nm
D_max	15.3	nm
Volume^Porod	256	nm³

SASDGF4 – Mixed lineage leukemia protein-1 complex, MLL1-WDR5-ASH2L-RBBP5(2-480)L399A/L400A/I457A/L459A

Sample:	Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein WD repeat-containing protein 5 monomer, 34 kDa Homo sapiens protein Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein Retinoblastoma-binding protein 5 monomer, 53 kDa Homo sapiens protein
Buffer:	300 mM NaCl, 25mM Tris-HCl, 4% glycerol, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Jun 22

R_g^Guinier	5.3	nm
D_max	17.2	nm
Volume^Porod	313	nm³

SASDGG4 – Mixed lineage leukemia protein-1 complex, MLL1-WDR5-ASH2L-RBBP5(2-538)

Sample:	Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein WD repeat-containing protein 5 monomer, 34 kDa Homo sapiens protein Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein Retinoblastoma-binding protein 5 monomer, 59 kDa Homo sapiens protein
Buffer:	300 mM NaCl, 25mM Tris-HCl, 4% glycerol, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Jun 22

R_g^Guinier	5.0	nm
D_max	15.5	nm
Volume^Porod	282	nm³

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