SASDGR9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mg/mL heparin (SEC-SAXS)

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 4.2 nm
Dmax 16.8 nm

SASDGS9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 2.4 nm
Dmax 8.3 nm

SASDGT9 – Truncated Apolipoprotein E4 (1-191) bound to 110 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 2.7 nm
Dmax 8.3 nm

SASDFJ3 – Maltose binding protein-SpoIVB peptidase fusion (MBP-SpoIVB)

SpoIVB peptidase (MBP fusion) experimental SAS data
CHIMERA model
Sample: SpoIVB peptidase (MBP fusion) monomer, 80 kDa Bacillus subtilis protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% glycerol, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Jul 12
Solution Structure of SpoIVB Reveals Mechanism of PDZ Domain-Regulated Protease Activity. Front Microbiol 10:1232 (2019)
Xie X, Guo N, Xue G, Xie D, Yuan C, Harrison J, Li J, Jiang L, Huang M
RgGuinier 3.7 nm
Dmax 15.6 nm
VolumePorod 96 nm3

SASDFV8 – Apolipoprotein E2 (ApoE2) bound to 4 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data
Apolipoprotein E2 Heparin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 6.2 nm
Dmax 20.7 nm
VolumePorod 504 nm3

SASDFW8 – Apolipoprotein E2 (ApoE2) bound to 12 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data
Apolipoprotein E2 Heparin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 6.4 nm
Dmax 21.7 nm
VolumePorod 531 nm3

SASDFX8 – Apolipoprotein E2 (ApoE2) bound to 37 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data
Apolipoprotein E2 Heparin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 6.5 nm
Dmax 23.4 nm
VolumePorod 584 nm3

SASDFY8 – Apolipoprotein E2 (ApoE2) bound to 110 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data
Apolipoprotein E2 Heparin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 7.1 nm
Dmax 24.0 nm
VolumePorod 666 nm3

SASDFZ8 – Apolipoprotein E2 (ApoE2) bound to 330 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data
Apolipoprotein E2 Heparin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 7.2 nm
Dmax 24.5 nm
VolumePorod 696 nm3

SASDG25 – DNA repair protein Rad5 (with 6xhis and twin-strep tags)

DNA repair protein RAD5 experimental SAS data
OTHER [STATIC IMAGE] model
Sample: DNA repair protein RAD5 monomer, 138 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris, 150 mM KCl, 5 mM DTT, 5% glycerol, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Mar 6
Conformational flexibility of fork-remodeling helicase Rad5 shown by full-ensemble hybrid methods. PLoS One 14(10):e0223875 (2019)
Gildenberg MS, Washington MT
RgGuinier 4.7 nm
Dmax 17.8 nm
VolumePorod 254 nm3

4728 hits found.