Structural role of essential light chains in the apicomplexan glideosome.

Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C, Commun Biol 3(1):568 (2020) Europe PMC

SASDH94 – Toxoplasma gondii myosin essential light chain 2 (TgELC2) bound to myosin A C-terminus

Myosin essential light chain 2
Myosin A
MWI(0) 16 kDa
MWexpected 18 kDa
VPorod 28 nm3
log I(s) 2.12×10-2 2.12×10-3 2.12×10-4 2.12×10-5
Myosin essential light chain 2 Myosin A small angle scattering data  s, nm-1
ln I(s)
Myosin essential light chain 2 Myosin A Guinier plot ln 2.13×10-2 Rg: 1.7 nm 0 (1.7 nm)-2 s2
(sRg)2I(s)/I(0)
Myosin essential light chain 2 Myosin A Kratky plot 1.104 0 3 sRg
p(r)
Myosin essential light chain 2 Myosin A pair distance distribution function Rg: 1.7 nm 0 Dmax: 5.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Myosin essential light chain 2 Myosin A PYMOL model

Synchrotron SAXS data from solutions of Toxoplasma gondii myosin essential light chain 2 (TgELC2) bound to myosin A C-terminus in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 90.00 μl sample at 10 mg/ml was injected at a 0.75 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20.1°C. 1500 successive 0.995 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Myosin essential light chain 2
Mol. type   Protein
Organism   Toxoplasma gondii
Olig. state   Monomer
Mon. MW   15.5 kDa
 
UniProt   B9PZ33 (1-133)
Sequence   FASTA
 
Myosin A (TgMyoA)
Mol. type   Protein
Organism   Toxoplasma gondii
Olig. state   Monomer
Mon. MW   2.6 kDa
 
UniProt   S8G527 (777-798)
Sequence   FASTA
 
PDB code   6TJ6