Structural role of essential light chains in the apicomplexan glideosome.

Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C, Commun Biol 3(1):568 (2020) Europe PMC

SASDHA4 – Trimeric complex of myosin A with myosin light chain (MLC1) and essential light chain (TgELC1) from Toxoplasma gondii

Toxoplasma gondii essential light chain 1
Myosin A
Toxoplasma gondii myosin light chain, full length
MWexperimental 38 kDa
MWexpected 44 kDa
VPorod 69 nm3
log I(s) 1.06×10-2 1.06×10-3 1.06×10-4 1.06×10-5
Toxoplasma gondii essential light chain 1 Myosin A Toxoplasma gondii myosin light chain, full length small angle scattering data  s, nm-1
ln I(s)
Toxoplasma gondii essential light chain 1 Myosin A Toxoplasma gondii myosin light chain, full length Guinier plot ln 1.07×10-2 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Toxoplasma gondii essential light chain 1 Myosin A Toxoplasma gondii myosin light chain, full length Kratky plot 1.104 0 3 sRg
p(r)
Toxoplasma gondii essential light chain 1 Myosin A Toxoplasma gondii myosin light chain, full length pair distance distribution function Rg: 3.5 nm 0 Dmax: 14 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Toxoplasma gondii essential light chain 1 Myosin A Toxoplasma gondii myosin light chain, full length PYMOL model

Synchrotron SAXS data from solutions of the trimeric complex between myosin A, the myosin light chain (MLC1) and the essential light chain (TgELC1) from Toxoplasma gondii in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 90.00 μl sample at 3.9 mg/ml was injected at a 0.80 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20.2°C. 1500 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Toxoplasma gondii essential light chain 1 (TgELC1)
Mol. type   Protein
Organism   Toxoplasma gondii
Olig. state   Monomer
Mon. MW   14.9 kDa
Sequence   FASTA
 
Myosin A (TgMyoA-C)
Mol. type   Protein
Organism   Toxoplasma gondii
Olig. state   Monomer
Mon. MW   5.0 kDa
 
UniProt   S8G527 (777-818)
Sequence   FASTA
 
Toxoplasma gondii myosin light chain, full length (TGMyoA)
Mol. type   Protein
Organism   Toxoplasma gondii
Olig. state   Monomer
Mon. MW   24.3 kDa
 
UniProt   Q95UJ7 (1-213)
Sequence   FASTA
 
PDB code   6TJ6