Structural role of essential light chains in the apicomplexan glideosome.

Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C, Commun Biol 3(1):568 (2020) Europe PMC

SASDHD4 – Trimeric complex of myosin A with myosin light chain (MLC1, residues 66-210) and essential light chain (TgELC2) from Toxoplasma gondii

Myosin essential light chain 2
Myosin light chain TgMLC1, residues 66-210
Myosin A
MWexperimental 35 kDa
MWexpected 38 kDa
VPorod 50 nm3
log I(s) 4.97×10-2 4.97×10-3 4.97×10-4 4.97×10-5
Myosin essential light chain 2 Myosin light chain TgMLC1, residues 66-210 Myosin A small angle scattering data  s, nm-1
ln I(s)
Myosin essential light chain 2 Myosin light chain TgMLC1, residues 66-210 Myosin A Guinier plot ln 4.97×10-2 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Myosin essential light chain 2 Myosin light chain TgMLC1, residues 66-210 Myosin A Kratky plot 1.104 0 3 sRg
p(r)
Myosin essential light chain 2 Myosin light chain TgMLC1, residues 66-210 Myosin A pair distance distribution function Rg: 2.7 nm 0 Dmax: 10 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Myosin essential light chain 2 Myosin light chain TgMLC1, residues 66-210 Myosin A PYMOL model

Synchrotron SAXS data from solutions of the trimeric complex between myosin A, the myosin light chain (MLC1, residues 66-210) and the essential light chain (TgELC2) from Toxoplasma gondii in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 85.00 μl sample at 15.5 mg/ml was injected at a 0.75 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20.2°C. 1800 successive 0.995 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Myosin essential light chain 2
Mol. type   Protein
Organism   Toxoplasma gondii
Olig. state   Monomer
Mon. MW   15.5 kDa
 
UniProt   B9PZ33 (1-133)
Sequence   FASTA
 
Myosin light chain TgMLC1, residues 66-210 (MLC1)
Mol. type   Protein
Organism   Toxoplasma gondii
Olig. state   Monomer
Mon. MW   17.2 kDa
 
UniProt   Q95UJ7 (66-210)
Sequence   FASTA
 
Myosin A (TgMyoA-C)
Mol. type   Protein
Organism   Toxoplasma gondii
Olig. state   Monomer
Mon. MW   5.0 kDa
 
UniProt   S8G527 (777-818)
Sequence   FASTA
 
PDB code   6TJ7