Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures

Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M, Molecules 27(21):7628 (2022) DOI

SASDQV7 – Bicelles formed by POPC POPG and CHAPS

CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine
POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol)
Temperature 20.0
log I(s)
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) small angle scattering data  s, nm-1
s2I(s)
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) Kratky plot 0 s

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Bicelles formed by POPC POPG and CHAPS in Tris buffered saline, pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 20°C. 20 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Data was collected on bicelles formed by POPC/POPG (80/20 Mol%) and CHAPS. The curve shown has a lipid-to-detergent ratio of 5 in Mol%, other ratios are available in the full entry.

Tags: softmatter
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate
Mol. type   Lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine
Mol. type   Lipid
POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol)
Mol. type   Lipid