Mechanistic insights into RNA binding and RNA-regulated RIG-I ubiquitination by TRIM25

Kevin Haubrich

Submitted to SASBDB: 2021 Jan 28 Published in SASBDB: 2021 Nov 24

SASDK78 – E3 ubiquitin/ISG15 ligase TRIM25, apo form (TRIM25 apo) Sample: E3 ubiquitin/ISG15 ligase TRIM25 dimer, 100 kDa Homo sapiens protein Buffer: 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH: 6.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 19 RgGuinier 6.8 nm Dmax 30.2 nm

SASDK88 – E3 ubiquitin/ISG15 ligase TRIM25 bound to pre-let-7-a-1@1 RNA (TRIM25/pre-let-7) Sample: E3 ubiquitin/ISG15 ligase TRIM25 dimer, 100 kDa Homo sapiens protein Pre-let-7-a-1@1 monomer, 9 kDa synthetic construct RNA Buffer: 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH: 6.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 14 RgGuinier 5.7 nm Dmax 19.8 nm

SASDK98 – E3 ubiquitin/ISG15 ligase TRIM25 bound to pre-let-7-a-1@1 RNA (TRIM25/pre-let-7): SEC-SAXS Sample: E3 ubiquitin/ISG15 ligase TRIM25 dimer, 100 kDa Homo sapiens protein Pre-let-7-a-1@1 monomer, 9 kDa synthetic construct RNA Buffer: 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 4 RgGuinier 5.7 nm Dmax 23.0 nm

SASDKA8 – E3 ubiquitin/ISG15 ligase TRIM25 bound to lnczc3h7a_304-326 RNA (TRIM25/lnczc3h7a) Sample: E3 ubiquitin/ISG15 ligase TRIM25 dimer, 100 kDa Homo sapiens protein Lnczc3h7a_304-326 monomer, 8 kDa Homo sapiens RNA Buffer: 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH: 6.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 12 RgGuinier 5.8 nm Dmax 16.6 nm