Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy.
Hawkins WD,
Leary KA,
Andhare D,
Popelka H,
Klionsky DJ,
Ragusa MJ
Cell Rep
39(3):110702
(2022 Apr 19)
|
|
|
Sample: |
Autophagy-related protein 23 dimer, 104 kDa Saccharomyces cerevisiae (strain … protein
|
Buffer: |
20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4 |
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 3
|
|
RgGuinier |
7.4 |
nm |
Dmax |
29.3 |
nm |
VolumePorod |
378 |
nm3 |
|
|
|
|
|
Sample: |
Autophagy-related protein 23 LIL Mutant monomer, 52 kDa Saccharomyces cerevisiae (strain … protein
|
Buffer: |
20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4 |
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 8
|
|
RgGuinier |
4.4 |
nm |
Dmax |
17.1 |
nm |
VolumePorod |
98 |
nm3 |
|
|
|
|
|
Sample: |
Autophagy-related protein 23 RKK Mutant dimer, 104 kDa Saccharomyces cerevisiae (strain … protein
|
Buffer: |
20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4 |
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 8
|
|
RgGuinier |
7.6 |
nm |
Dmax |
34.5 |
nm |
VolumePorod |
365 |
nm3 |
|
|