Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy.

Hawkins WD, Leary KA, Andhare D, Popelka H, Klionsky DJ, Ragusa MJ
Cell Rep 39(3):110702 (2022 Apr 19)
PMID: 35443167
doi: 10.1016/j.celrep.2022.110702
Submitted to SASBDB: 2021 Jul 6
Published in SASBDB:

SASDL39 – Autophagy-related protein 23

Autophagy-related protein 23 experimental SAS data
Autophagy-related protein 23 Kratky plot
Sample: Autophagy-related protein 23 dimer, 104 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 3
RgGuinier 7.4 nm
Dmax 29.3 nm
VolumePorod 378 nm3

SASDL49 – Autophagy-related protein 23 L171A,I182A,L189A mutant

Autophagy-related protein 23 LIL Mutant experimental SAS data
Autophagy-related protein 23 LIL Mutant Kratky plot
Sample: Autophagy-related protein 23 LIL Mutant monomer, 52 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 8
RgGuinier 4.4 nm
Dmax 17.1 nm
VolumePorod 98 nm3

SASDL59 – Autophagy-related protein 23 R190A,K193A,K194A mutant

Autophagy-related protein 23 RKK Mutant experimental SAS data
Autophagy-related protein 23 RKK Mutant Kratky plot
Sample: Autophagy-related protein 23 RKK Mutant dimer, 104 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 8
RgGuinier 7.6 nm
Dmax 34.5 nm
VolumePorod 365 nm3