The BAM7 gene in Zea mays encodes a protein with similar structural and catalytic properties to Arabidopsis BAM2

Ravenburg C, Riney M, Monroe J, Berndsen C
Acta Crystallographica Section D Structural Biology 78(5) (2022 Apr 08)

doi: 10.1107/S2059798322002169

Submitted to SASBDB: 2021 Oct 11 Published in SASBDB: 2022 Apr 8

SASDMX5 – Zea mays beta-amylase (ZmBAM7) short Sample: Beta-amylase tetramer, 231 kDa Zea mays protein Buffer: 50 mM HEPES, 25 mM NaCl, and 0.2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 7 RgGuinier 5.3 nm Dmax 16.3 nm VolumePorod 615 nm3

SASDMY5 – Truncated Zea mays beta-amylase (ZmBAM7) short Sample: Beta-amylase tetramer, 231 kDa Zea mays protein Buffer: 50 mM HEPES, 25 mM NaCl, and 0.2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 7 RgGuinier 5.1 nm Dmax 13.9 nm VolumePorod 467 nm3

SASDMZ5 – Arabidopsis thaliana beta-amylase 1 Sample: Beta-amylase 1, chloroplastic monomer, 65 kDa Arabidopsis thaliana protein Buffer: 50 mM MES, 100 mM NaCl, 1 mM DTT, pH: 6.7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 22 RgGuinier 2.6 nm Dmax 9.8 nm VolumePorod 88 nm3

SASDM26 – Sweet potato beta-amylase 5 Sample: Beta-amylase tetramer, 224 kDa Ipomoea batatas protein Buffer: 20 mM HEPES, 150 mM NaCl, and 0.2 mM TCEP, pH: 7.3 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 7 RgGuinier 4.4 nm Dmax 14.1 nm VolumePorod 296 nm3