Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase.

Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME
Biophys J (2022 Apr 20)

PMID: 35443926 doi: 10.1016/j.bpj.2022.04.021

Submitted to SASBDB: 2021 Nov 9 Published in SASBDB: 2021 Nov 10

SASDMJ8 – Sulfite reductase dodecamer Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3 RgGuinier 11.0 nm Dmax 32.5 nm VolumePorod 1720 nm3

SASDMK8 – Sulfite reductase flavoprotein octamer Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3 RgGuinier 8.6 nm Dmax 26.0 nm VolumePorod 830 nm3

SASDML8 – Sulfite reductase hemoprotein Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2018 Jul 13 RgGuinier 2.3 nm Dmax 7.2 nm VolumePorod 71 nm3

SASDMM8 – Reconstituted sulfite reductase dodecamer with partially deuterated hemoprotein in 0% D2O Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3 RgGuinier 11.2 nm Dmax 30.5 nm

SASDMN8 – Reconstituted sulfite reductase dodecamer with partially deuterated hemoprotein in 20% D2O Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3 RgGuinier 11.3 nm Dmax 29.5 nm

SASDMP8 – Reconstituted sulfite reductase dodecamer with partially deuterated hemoprotein in 41% D2O Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3 RgGuinier 11.4 nm Dmax 28.5 nm VolumePorod 417 nm3

SASDMQ8 – Reconstituted sulfite reductase dodecamer with partially deuterated hemoprotein in 86% D2O Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3 RgGuinier 6.8 nm Dmax 22.0 nm VolumePorod 460 nm3

SASDMR8 – Reconstituted sulfite reductase dodecamer with partially deuterated hemoprotein in 100% D2O Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) octamer, 565 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3 RgGuinier 8.5 nm Dmax 25.1 nm

SASDMS8 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein heterodimer in 0% D2O Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3 RgGuinier 3.4 nm Dmax 13.0 nm

SASDMT8 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein heterodimer in 41% D2O Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3 RgGuinier 2.2 nm Dmax 6.6 nm

SASDMU8 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein heterodimer in 86% D2O Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3 RgGuinier 3.1 nm Dmax 12.4 nm

SASDMV8 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein heterodimer in 100% D2O Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2021 Apr 3 RgGuinier 4.0 nm Dmax 13.9 nm