Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments.

Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI
Nat Protoc 11(11):2122-2153 (2016 Nov)
PMID: 27711050
doi: 10.1038/nprot.2016.113
Submitted to SASBDB: 2016 Jan 25
Published in SASBDB:

SASDBJ3 – Bovine serum albumin, monomer from SEC-SAXS

Bovine serum albumin, monomer experimental SAS data
PDB model
Sample: Bovine serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 100 nm3

SASDBK3 – Bovine serum albumin, dimer from SEC-SAXS

Bovine serum albumin, dimer experimental SAS data
PDB model
Sample: Bovine serum albumin, dimer dimer, 133 kDa Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
RgGuinier 3.9 nm
Dmax 12.7 nm
VolumePorod 202 nm3