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335 hits found for Svergun

SASDD32 – DNA-(adenine N6)-methyltransferase from Acinetobacter baumannii ATCC 17978

DNA-(adenine N6)-methyltransferase experimental SAS data
DAMMIN model
Sample: DNA-(adenine N6)-methyltransferase monomer, 49 kDa Acinetobacter baumannii protein
Buffer: 150mM NaCl, 10mM Tris, 1mM DTT, 5% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Sep 30
Recombinant production of A1S_0222 from Acinetobacter baumannii ATCC 17978 and confirmation of its DNA-(adenine N6)-methyltransferase activity. Protein Expr Purif 151:78-85 (2018)
...Svergun DI, Wilharm G
RgGuinier 2.9 nm
Dmax 11.1 nm
VolumePorod 96 nm3

SASDD42 – Synechocystis fluorescence recovery protein SynFRP.8-109

Fluorescence recovery protein experimental SAS data
GASBOR model
Sample: Fluorescence recovery protein dimer, 23 kDa Synechocystis sp. PCC … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 1
Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP. Biochim Biophys Acta 1859(5):382-393 (2018)
...Svergun DI, Friedrich T, Sluchanko NN
RgGuinier 2.8 nm
Dmax 10.5 nm
VolumePorod 36 nm3

SASDB52 – Death associated protein kinase (wild-type)

Death associated protein kinase wild-type experimental SAS data
NONE model
Sample: Death associated protein kinase wild-type , 37 kDa Homo sapiens protein
Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
...Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
RgGuinier 2.7 nm
Dmax 8.1 nm
VolumePorod 101 nm3

SASDC52 – Bruton tyrosine kinase

Tyrosine-protein kinase BTK (R28C mutant) experimental SAS data
GASBOR model
Sample: Tyrosine-protein kinase BTK (R28C mutant) monomer, 76 kDa Homo sapiens protein
Buffer: 20 mM HEPES 200 mM NaCl, 2 mM DTT and 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2002 Apr 2
Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering. EMBO J 22(18):4616-24 (2003)
...Svergun DI
RgGuinier 5.0 nm
Dmax 20.0 nm
VolumePorod 130 nm3

SASDD52 – Arthrospira fluorescence recovery protein AmaxFRP.1-106

Uncharacterized fluorescence recovery protein experimental SAS data
GASBOR model
Sample: Uncharacterized fluorescence recovery protein dimer, 24 kDa Arthrospira maxima CS-328 protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 1
Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP. Biochim Biophys Acta 1859(5):382-393 (2018)
...Svergun DI, Friedrich T, Sluchanko NN
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDB62 – Death associated protein kinase (D220K mutant)

Death associated protein kinase (D220K mutant) experimental SAS data
NONE model
Sample: Death associated protein kinase (D220K mutant) , 37 kDa Homo sapiens protein
Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
...Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 89 nm3

SASDB72 – Death associated protein kinase (Basic Loop mutant)

Death associated protein kinase (Basic Loop mutant) experimental SAS data
NONE model
Sample: Death associated protein kinase (Basic Loop mutant) , 37 kDa Homo sapiens protein
Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
...Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
RgGuinier 2.4 nm
Dmax 7.5 nm
VolumePorod 78 nm3

SASDH92 – Plasmodium falciparum lipocalin (PF3D7_0925900): Dimer-tetramer equilibrium through a concentration series (combined batch and SEC-SAXS measurements)

Plasmodium falciparum Lipocalin experimental SAS data
SASREF MX model
Sample: Plasmodium falciparum Lipocalin tetramer, 89 kDa Plasmodium falciparum protein
Buffer: 20 mM Tris pH7.5, 150 mM NaCl, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 8
Structure-Based Identification and Functional Characterization of a Lipocalin in the Malaria Parasite Plasmodium falciparum Cell Reports 31(12):107817 (2020)
...Svergun D, Wilson D, Wilmanns M, Gilberger T
RgGuinier 3.2 nm
Dmax 10.3 nm
VolumePorod 126 nm3

SASDED2 – Polyglutamine tract-binding protein 1 (PQBP-1)

Polyglutamine-binding protein 1 experimental SAS data
Polyglutamine tract-binding protein 1 (PQBP-1) Rg histogram
Sample: Polyglutamine-binding protein 1 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 1mM DTT,, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III on 2009 Nov 18
Solution model of the intrinsically disordered polyglutamine tract-binding protein-1. Biophys J 102(7):1608-16 (2012)
...Svergun D, Chen YW
RgGuinier 3.7 nm
Dmax 13.0 nm
VolumePorod 51 nm3

SASDGD2 – Putative mitochondrial transferase CAF17: human IBA57

Putative transferase CAF17, mitochondrial experimental SAS data
PDB model
Sample: Putative transferase CAF17, mitochondrial monomer, 33 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 10
Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery Scientific Reports 9(1) (2019)
...Svergun D, Banci L
RgGuinier 2.2 nm
Dmax 7.1 nm
VolumePorod 50 nm3

SASDGE2 – Human mitochondrial iron-sulfur cluster assembly ISCA2 protein

Iron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data
SASREF model
Sample: Iron-sulfur cluster assembly 2 homolog, mitochondrial dimer, 24 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 16
Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery Scientific Reports 9(1) (2019)
...Svergun D, Banci L
RgGuinier 2.0 nm
Dmax 6.7 nm
VolumePorod 31 nm3

SASDGF2 – Putative mitochondrial transferase CAF17 bound to mitochondrial iron-sulfur cluster assembly ISCA2 protein (human IBA57-ISCA2 complex)

Putative transferase CAF17, mitochondrialIron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data
SASREF CV model
Sample: Putative transferase CAF17, mitochondrial monomer, 33 kDa Homo sapiens protein
Iron-sulfur cluster assembly 2 homolog, mitochondrial dimer, 24 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 22
Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery Scientific Reports 9(1) (2019)
...Svergun D, Banci L
RgGuinier 4.0 nm
Dmax 16.4 nm
VolumePorod 114 nm3

SASDEH2 – Unlabeled nucleoporin NUP49/NSP49 (N49) without denaturant

Nucleoporin NUP49/NSP49 experimental SAS data
Unlabeled nucleoporin NUP49/NSP49 (N49) without denaturant Rg histogram
Sample: Nucleoporin NUP49/NSP49 monomer, 4 kDa Saccharomyces cerevisiae protein
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 4 nm3

SASDEJ2 – Labeled nucleoporin NUP49/NSP49 (N49-Alexa488/Alexa594) without denaturant

Nucleoporin NUP49/NSP49Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Nucleoporin NUP49/NSP49 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Nucleoporin NUP49/NSP49 monomer, 4 kDa Saccharomyces cerevisiae protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 1.9 nm
Dmax 6.5 nm
VolumePorod 12 nm3

SASDCK2 – Glucose Isomerase - Streptomyces rubiginosus

Xylose isomerase experimental SAS data
DAMMIN model
Sample: Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
...Svergun DI, Sugiyama M, Tainer JA, Vachette P, Westbrook J, Whitten AE
RgGuinier 3.3 nm
Dmax 9.2 nm
VolumePorod 229 nm3

SASDEK2 – Unlabeled nucleoporin NUP49/NSP49 (N49) with denaturant

Nucleoporin NUP49/NSP49 experimental SAS data
Unlabeled nucleoporin NUP49/NSP49 (N49) with denaturant Rg histogram
Sample: Nucleoporin NUP49/NSP49 monomer, 4 kDa Saccharomyces cerevisiae protein
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 1.7 nm
Dmax 6.5 nm
VolumePorod 4 nm3

SASDEL2 – Labeled nucleoporin NUP49/NSP49 (N49-Alexa488/Alexa594) with denaturant

Nucleoporin NUP49/NSP49Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Nucleoporin NUP49/NSP49 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Nucleoporin NUP49/NSP49 monomer, 4 kDa Saccharomyces cerevisiae protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.1 nm
Dmax 7.7 nm
VolumePorod 7 nm3

SASDEM2 – Unlabeled Nuclear Localization Signal (NLS) from the inner nuclear membrane protein HEH2 without denaturant

Inner nuclear membrane protein HEH2 experimental SAS data
Unlabeled Nuclear Localization Signal (NLS) from the inner nuclear membrane protein HEH2 without denaturant Rg histogram
Sample: Inner nuclear membrane protein HEH2 monomer, 5 kDa Saccharomyces cerevisiae protein
Buffer: 25 mM HEPES, 150 mM NaCl, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 25
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.4 nm
Dmax 11.6 nm
VolumePorod 16 nm3

SASDEN2 – Labeled Nuclear Localization Signal from the inner nuclear membrane protein HEH2 (NLS-Alexa488/Alexa594) without denaturant

Inner nuclear membrane protein HEH2Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Inner nuclear membrane protein HEH2 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Inner nuclear membrane protein HEH2 monomer, 5 kDa Saccharomyces cerevisiae protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: 25 mM HEPES, 150 mM NaCl, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 25
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.0 nm
Dmax 7.3 nm
VolumePorod 10 nm3

SASDEP2 – Unlabeled Nuclear Localization Signal (NLS) from the inner nuclear membrane protein HEH2 with denaturant

Inner nuclear membrane protein HEH2 experimental SAS data
Unlabeled Nuclear Localization Signal (NLS) from the inner nuclear membrane protein HEH2 with denaturant Rg histogram
Sample: Inner nuclear membrane protein HEH2 monomer, 5 kDa Saccharomyces cerevisiae protein
Buffer: 25 mM HEPES, 150 mM NaCl, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 25
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.3 nm
Dmax 7.1 nm
VolumePorod 10 nm3

SASDCQ2 – 4Ca2+-calmodulin - Xenopus laevis

Calmodulin experimental SAS data
4Ca2+-calmodulin - Xenopus laevis Rg histogram
Sample: Calmodulin monomer, 17 kDa Xenopus laevis protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
...Svergun DI, Sugiyama M, Tainer JA, Vachette P, Westbrook J, Whitten AE
RgGuinier 2.2 nm
Dmax 7.2 nm
VolumePorod 25 nm3

SASDEQ2 – Labeled Nuclear Localization Signal from the inner nuclear membrane protein HEH2 (NLS-Alexa488/Alexa594) with denaturant

Inner nuclear membrane protein HEH2Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Inner nuclear membrane protein HEH2 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Inner nuclear membrane protein HEH2 monomer, 5 kDa Saccharomyces cerevisiae protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: 25 mM HEPES, 150 mM NaCl, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 25
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.4 nm
Dmax 8.1 nm
VolumePorod 12 nm3

SASDER2 – Unlabeled Importin Beta Binding domain (IBB) from importin subunit alpha-1 without denaturant

Importin subunit alpha-1 experimental SAS data
Unlabeled Importin Beta Binding domain (IBB) from importin subunit alpha-1 without denaturant Rg histogram
Sample: Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 30 nm3

SASDES2 – Labeled Importin Beta Binding Domain (IBB-Alexa488/Alexa594) from importin subunit alpha-1 without denaturant

Importin subunit alpha-1Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Importin subunit alpha-1 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 9
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.9 nm
Dmax 13.7 nm
VolumePorod 29 nm3

SASDET2 – Unlabeled Importin Beta Binding Domain (IBB) from importin subunit alpha-1 with denaturant

Importin subunit alpha-1 experimental SAS data
Unlabeled Importin Beta Binding Domain (IBB) from importin subunit alpha-1 with denaturant Rg histogram
Sample: Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 3.1 nm
Dmax 15.3 nm
VolumePorod 34 nm3

SASDEU2 – Labeled Importin Beta Binding Domain from importin subunit alpha-1 (IBB-Alexa488/Alexa594) with denaturant

Importin subunit alpha-1Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Importin subunit alpha-1 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 3.2 nm
Dmax 13.3 nm
VolumePorod 29 nm3

SASDEV2 – Unlabeled nuclear pore complex protein Nup153 (NUS) without denaturant

Nuclear pore complex protein Nup153 experimental SAS data
Unlabeled nuclear pore complex protein Nup153 (NUS) without denaturant Rg histogram
Sample: Nuclear pore complex protein Nup153 monomer, 8 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.5 nm
Dmax 9.2 nm
VolumePorod 15 nm3

SASDEW2 – Labeled nuclear pore complex protein Nup153 (NUS-Alexa488/Alexa594) without denaturant

Nuclear pore complex protein Nup153Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Nuclear pore complex protein Nup153 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Nuclear pore complex protein Nup153 monomer, 8 kDa Homo sapiens protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.5 nm
Dmax 9.7 nm
VolumePorod 17 nm3

SASDAX2 – Pyruvate decarboxylase (PDC) from Z. mobilis

Pyruvate decarboxylase experimental SAS data
CRYSOL model
Sample: Pyruvate decarboxylase tetramer, 244 kDa Zymomonas mobilis protein
Buffer: 100 mM Sodium Citrate, 17% Glycerol, 22.5% PEG 1500, pH: 6
Experiment: SAXS data collected at EMBL X33, DORIS III on 1998 Nov 3
Crystal versus solution structures of thiamine diphosphate-dependent enzymes. J Biol Chem 275(1):297-302 (2000)
Svergun DI, Petoukhov MV, Koch MH, König S
RgGuinier 3.9 nm
Dmax 11.0 nm

SASDEX2 – Unlabeled nuclear pore complex protein Nup153 (NUS) with denaturant

Nuclear pore complex protein Nup153 experimental SAS data
Unlabeled nuclear pore complex protein Nup153 (NUS) with denaturant Rg histogram
Sample: Nuclear pore complex protein Nup153 monomer, 8 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 3.1 nm
Dmax 11.0 nm
VolumePorod 26 nm3

SASDBY2 – Inorganic pyrophosphatase (PPase) from E. coli

Inorganic pyrophosphatase (PPase) from E. coli experimental SAS data
DAMMIN model
Sample: Inorganic pyrophosphatase (PPase) from E. coli hexamer, 117 kDa Escherichia coli protein
Buffer: 50 mM Tris 10 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 30
X-Ray Solution Scattering Study of Four Escherichia coli Enzymes Involved in Stationary-Phase Metabolism. PLoS One 11(5):e0156105 (2016)
...Svergun DI
RgGuinier 3.0 nm
Dmax 9.0 nm
VolumePorod 166 nm3

SASDEY2 – Labeled nuclear pore complex protein Nup153 (NUS-Alexa488/Alexa594) with denaturant

Nuclear pore complex protein Nup153Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Nuclear pore complex protein Nup153 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Nuclear pore complex protein Nup153 monomer, 8 kDa Homo sapiens protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.9 nm
Dmax 10.8 nm
VolumePorod 19 nm3

SASDBZ2 – Class I fructose-1,6-bisphosphate aldolase (FbaB) from E. coli

Class I fructose-1,6-bisphosphate aldolase (FbaB) from E. coli experimental SAS data
DAMMIN model
Sample: Class I fructose-1,6-bisphosphate aldolase (FbaB) from E. coli decamer, 381 kDa Escherichia coli protein
Buffer: 50 mM Tris 10 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 30
X-Ray Solution Scattering Study of Four Escherichia coli Enzymes Involved in Stationary-Phase Metabolism. PLoS One 11(5):e0156105 (2016)
...Svergun DI
RgGuinier 4.4 nm
Dmax 12.7 nm
VolumePorod 484 nm3

SASDEZ2 – Unlabeled nuclear pore complex protein Nup153 (NUL) without denaturant

Nuclear pore complex protein Nup153 experimental SAS data
Unlabeled nuclear pore complex protein Nup153 (NUL) without denaturant Rg histogram
Sample: Nuclear pore complex protein Nup153 monomer, 12 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 3.0 nm
Dmax 10.9 nm
VolumePorod 32 nm3

SASDB23 – 5-keto-4-deoxyuronate isomerase (KduI) from E. coli

5-keto-4-deoxyuronate isomerase (KduI) from E. coli experimental SAS data
NONE model
Sample: 5-keto-4-deoxyuronate isomerase (KduI) from E. coli None, Escherichia coli protein
Buffer: 50 mM Tris 10 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 20
X-Ray Solution Scattering Study of Four Escherichia coli Enzymes Involved in Stationary-Phase Metabolism. PLoS One 11(5):e0156105 (2016)
...Svergun DI
RgGuinier 4.5 nm

SASDE23 – Labeled nuclear pore complex protein Nup153 (NUL-Alexa488/Alexa594) without denaturant

Nuclear pore complex protein Nup153Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Nuclear pore complex protein Nup153 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Nuclear pore complex protein Nup153 monomer, 12 kDa Homo sapiens protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 3.1 nm
Dmax 12.0 nm
VolumePorod 24 nm3

SASDB33 – Glutamate decarboxylase alpha (GadA) from E. coli

Glutamate decarboxylase alpha (GadA) from E. coli experimental SAS data
SASREF MX model
Sample: Glutamate decarboxylase alpha (GadA) from E. coli monomer, 53 kDa Escherichia coli protein
Buffer: 50 mM Tris 10 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 20
X-Ray Solution Scattering Study of Four Escherichia coli Enzymes Involved in Stationary-Phase Metabolism. PLoS One 11(5):e0156105 (2016)
...Svergun DI
RgGuinier 4.8 nm
VolumePorod 410 nm3

SASDD33 – Toxin/Antitoxin complex from M. tuberculosis

Mycobacterial cidal toxinMycobacterial cidal antitoxin experimental SAS data
PDB model
Sample: Mycobacterial cidal toxin hexamer, 121 kDa Mycobacterium tuberculosis protein
Mycobacterial cidal antitoxin hexamer, 76 kDa Mycobacterium tuberculosis protein
Buffer: 100 mM HEPES, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
An NAD+ Phosphorylase Toxin Triggers Mycobacterium tuberculosis Cell Death. Mol Cell (2019)
...Svergun DI, Schnappinger D, Schneider TR, Genevaux P, de Carvalho LPS, Wilmanns M, Parret AHA, Neyrolles O
RgGuinier 4.1 nm
Dmax 11.4 nm
VolumePorod 262 nm3

SASDE33 – Unlabeled nuclear pore complex protein Nup153 (NUL) with denaturant

Nuclear pore complex protein Nup153 experimental SAS data
Unlabeled nuclear pore complex protein Nup153 (NUL) with denaturant Rg histogram
Sample: Nuclear pore complex protein Nup153 monomer, 12 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 3.5 nm
Dmax 16.0 nm
VolumePorod 53 nm3

SASDE43 – Labeled nuclear pore complex protein Nup153 (NUL-Alexa488/Alexa594) with denaturant

Nuclear pore complex protein Nup153Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Nuclear pore complex protein Nup153 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Nuclear pore complex protein Nup153 monomer, 12 kDa Homo sapiens protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 27 nm3

SASDE53 – Unlabeled dihydrolipoyllysine-residue succinyltransferase component (BBL) with denaturant

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex experimental SAS data
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex Kratky plot
Sample: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex monomer, 4 kDa Escherichia coli protein
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.1 nm
Dmax 8.3 nm
VolumePorod 41 nm3

SASDF53 – Bruton's Tyrosine Kinase - kinase domain

Bruton's tyrosine kinase, kinase domain experimental SAS data
DAMMIN model
Sample: Bruton's tyrosine kinase, kinase domain monomer, 32 kDa Homo sapiens protein
Buffer: 20mM Tris, 150mM NaCl, 1mM TCEP, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 11
Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms Nature Communications 11(1) (2020)
...Svergun D, Kim H, Dal Peraro M, Hantschel O
RgGuinier 2.1 nm
Dmax 6.8 nm
VolumePorod 52 nm3

SASDE63 – Labeled dihydrolipoyllysine-residue succinyltransferase component (BBL-Alexa488/Alexa594) with denaturant

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complexAlexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex monomer, 4 kDa Escherichia coli protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.3 nm
Dmax 10.7 nm
VolumePorod 13 nm3

SASDF63 – Bruton's Tyrosine Kinase - SH2-kinase domain

Bruton's tyrosine kinase - Src homolgy domain 2 - kinase domain experimental SAS data
Bruton's Tyrosine Kinase - SH2-kinase domain Rg histogram
Sample: Bruton's tyrosine kinase - Src homolgy domain 2 - kinase domain monomer, 46 kDa Homo sapiens protein
Buffer: 20mM Tris, 150mM NaCl, 1mM TCEP, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Nov 3
Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms Nature Communications 11(1) (2020)
...Svergun D, Kim H, Dal Peraro M, Hantschel O
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 64 nm3

SASDE73 – Unlabeled cold shock protein (CSP) with denaturant

Cold shock-like protein experimental SAS data
Cold shock-like protein Kratky plot
Sample: Cold shock-like protein monomer, 7 kDa Thermotoga maritima protein
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 16
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.5 nm
Dmax 11.5 nm
VolumePorod 17 nm3

SASDF73 – Bruton's Tyrosine Kinase - SH3-SH2-kinase domain

Bruton's tyrosine kinase - Src homology 3-2 kinase domain experimental SAS data
Bruton's Tyrosine Kinase - SH3-SH2-kinase domain Rg histogram
Sample: Bruton's tyrosine kinase - Src homology 3-2 kinase domain monomer, 52 kDa Homo sapiens protein
Buffer: 20mM Tris, 150mM NaCl, 1mM TCEP, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Nov 2
Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms Nature Communications 11(1) (2020)
...Svergun D, Kim H, Dal Peraro M, Hantschel O
RgGuinier 2.6 nm
Dmax 8.3 nm
VolumePorod 72 nm3

SASDE83 – Labeled cold shock protein (CSP-Alexa488/Alexa594) with denaturant

Cold shock-like proteinAlexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Cold shock-like protein Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Cold shock-like protein monomer, 7 kDa Thermotoga maritima protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.2 nm
Dmax 8.3 nm
VolumePorod 9 nm3

SASDF83 – Bruton's Tyrosine Kinase - Full-length

Bruton's tyrosine kinase - full length experimental SAS data
Bruton's Tyrosine Kinase - Full-length Rg histogram
Sample: Bruton's tyrosine kinase - full length monomer, 77 kDa Homo sapiens protein
Buffer: 20mM Tris, 150mM NaCl, 1mM TCEP, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Nov 3
Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms Nature Communications 11(1) (2020)
...Svergun D, Kim H, Dal Peraro M, Hantschel O
RgGuinier 4.0 nm
Dmax 15.6 nm
VolumePorod 114 nm3

SASDE93 – Unlabeled thioredoxin (TRX) with denaturant

Thioredoxin 1 experimental SAS data
Thioredoxin 1 Kratky plot
Sample: Thioredoxin 1 monomer, 12 kDa Escherichia coli protein
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 3.6 nm
Dmax 13.0 nm
VolumePorod 34 nm3

SASDEA3 – Labeled thioredoxin (TRX-Alexa488/Alexa594) with denaturant

Thioredoxin 1Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Thioredoxin 1 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Thioredoxin 1 monomer, 12 kDa Escherichia coli protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 3.2 nm
Dmax 13.9 nm
VolumePorod 31 nm3

SASDFA3 – Human ATP-citrate synthase (ACLY) full length in HBS

ATP-citrate synthase experimental SAS data
ATP-citrate synthase Kratky plot
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.1 nm
Dmax 19.0 nm
VolumePorod 765 nm3

SASDEB3 – Unlabeled nuclear pore complex protein Nup98-Nup96 (N98) without denaturant

Nuclear pore complex protein Nup98-Nup96 experimental SAS data
Nuclear pore complex protein Nup98-Nup96 Kratky plot
Sample: Nuclear pore complex protein Nup98-Nup96 monomer, 15 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 24
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 2.9 nm
Dmax 10.4 nm
VolumePorod 27 nm3

SASDFB3 – Human ATP-citrate synthers (ACLY) full length in HBS + Citrate

ATP-citrate synthase experimental SAS data
ATP-citrate synthase Kratky plot
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.2 nm
Dmax 19.0 nm
VolumePorod 787 nm3

SASDBC3 – RepB, the replication initiator protein of a promiscuous Streptococcal plasmid pMV158

Replication initiator protein of a promiscuous streptococcal plasmid pMV158. experimental SAS data
NONE model
Sample: Replication initiator protein of a promiscuous streptococcal plasmid pMV158. hexamer, Streptococcus sp. protein
Buffer: 10 mM TRIS 5 mM EDTA 1.0 M KCl, pH: 8.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2007 Jun 16
Conformational plasticity of RepB, the replication initiator protein of promiscuous streptococcal plasmid pMV158. Sci Rep 6:20915 (2016)
...Svergun DI, Orozco M, del Solar G, Coll M
RgGuinier 3.8 nm
Dmax 10.6 nm
VolumePorod 282 nm3

SASDEC3 – Unlabeled nucleoporin NSP1 (NSP) without denaturant

Nucleoporin NSP1 experimental SAS data
Nucleoporin NSP1 Kratky plot
Sample: Nucleoporin NSP1 monomer, 18 kDa Saccharomyces cerevisiae protein
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 24
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
...Svergun DI, Lemke EA
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 45 nm3

SASDFC3 – Human ATP-citrate synthase (ACLY) full length in HBS + Citrate + Coenzyme-A

ATP-citrate synthase experimental SAS data
MULTIFOXS model
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 5.9 nm
Dmax 17.0 nm
VolumePorod 775 nm3

SASDBJ3 – Bovine serum albumin, monomer from SEC-SAXS

Bovine serum albumin, monomer experimental SAS data
PDB model
Sample: Bovine serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
...Svergun DI
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 100 nm3

SASDCJ3 – Bovine Serum Albumin

Serum albumin experimental SAS data
DAMMIN model
Sample: Serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
...Svergun DI, Sugiyama M, Tainer JA, Vachette P, Westbrook J, Whitten AE
RgGuinier 2.8 nm
Dmax 8.7 nm
VolumePorod 101 nm3

SASDDJ3 – Candida antarctica lipase B - with guanidine-HCl unfolding series

Lipase B from Pseudozyma antarctica experimental SAS data
Lipase B from Pseudozyma antarctica Kratky plot
Sample: Lipase B from Pseudozyma antarctica , 33 kDa Moesziomyces antarcticus protein
Buffer: 100 mM NaCl, 20 mM Na2HPO4, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
...Svergun DI
RgGuinier 2.4 nm

SASDBK3 – Bovine serum albumin, dimer from SEC-SAXS

Bovine serum albumin, dimer experimental SAS data
PDB model
Sample: Bovine serum albumin, dimer dimer, 133 kDa Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
...Svergun DI
RgGuinier 3.9 nm
Dmax 12.7 nm
VolumePorod 202 nm3

SASDDK3 – Candida antarctica lipase B - with guanidine-HCl unfolding series, in the presence of dithiothreitol

Lipase B from Pseudozyma antarctica experimental SAS data
Lipase B from Pseudozyma antarctica Kratky plot
Sample: Lipase B from Pseudozyma antarctica , 33 kDa Moesziomyces antarcticus protein
Buffer: 100 mM NaCl, 20 mM Na2HPO4, 10 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
...Svergun DI
RgGuinier 2.4 nm

SASDDL3 – Folded ribonuclease A (RNAse)

Ribonuclease pancreatic experimental SAS data
PDB model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: phosphate buffered saline (PBS), pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
...Svergun DI
RgGuinier 1.6 nm
Dmax 5.6 nm
VolumePorod 16 nm3

SASDDM3 – Carboyxamidomethylated ribonuclease A (unfolded RNAse) - with and without urea

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: 10 mM HCl, pH: 1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
...Svergun DI
RgGuinier 2.3 nm
Dmax 9.0 nm

SASDAN3 – MutS dimer

DNA mismatch repair protein MutS experimental SAS data
DAMMIF model
Sample: DNA mismatch repair protein MutS dimer, 191 kDa Escherichia coli protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Feb 28
Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation. Nucleic Acids Res 41(17):8166-81 (2013)
...Svergun DI, Friedhoff P, Sixma TK
RgGuinier 4.7 nm
Dmax 15.5 nm
VolumePorod 307 nm3

SASDDN3 – Bovine serum albumin mixture: averaged and individual data frames (subtracted and unsubtracted test sets)

Bovine serum albumin experimental SAS data
Bovine serum albumin Kratky plot
Sample: Bovine serum albumin , 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Sep 25
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
...Svergun DI
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 117 nm3

SASDAQ3 – MutS tetramer

DNA mismatch repair protein MutS experimental SAS data
DAMMIF model
Sample: DNA mismatch repair protein MutS tetramer, 381 kDa Escherichia coli protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 May 12
Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation. Nucleic Acids Res 41(17):8166-81 (2013)
...Svergun DI, Friedhoff P, Sixma TK
RgGuinier 7.8 nm
Dmax 28.0 nm
VolumePorod 700 nm3

SASDAR3 – PsrP functional binding region

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein experimental SAS data
PsrP functional binding region Rg histogram
Sample: Functional binding region (187-385) of the pneumococcal serine-rich repeat protein monomer, 22 kDa Streptococcus pneumoniae protein
Buffer: 20 mM sodium citrate 250 mM NaCl 2.5 % Glycerol, pH: 5.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 Jul 2
The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold. Open Biol 4:130090 (2014)
...Svergun D, Henriques-Normark B, Achour A
RgGuinier 2.3 nm
Dmax 7.7 nm
VolumePorod 37 nm3

SASDAS3 – Fila16-17

immunoglobulin- like filamin two-domain fragment 16-17 experimental SAS data
GASBOR model
Sample: immunoglobulin- like filamin two-domain fragment 16-17 monomer, 19 kDa Escherichia coli protein
Buffer: 100 mM NaCl 10 mM dithiothreitol 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2007 Jun 18
Atomic structures of two novel immunoglobulin-like domain pairs in the actin cross-linking protein filamin. J Biol Chem 284(37):25450-8 (2009)
...Svergun DI, Iivanainen T, Heikkinen SM, Permi P, Koskela H, Kilpeläinen I, Ylänne J
RgGuinier 1.9 nm
Dmax 6.0 nm
VolumePorod 33 nm3

SASDAT3 – Fila18-19

immunoglobulin- like filamin two-domain fragment 18-19 experimental SAS data
GASBOR model
Sample: immunoglobulin- like filamin two-domain fragment 18-19 monomer, 20 kDa Escherichia coli protein
Buffer: 100 mM NaCl 10 mM dithiothreitol 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2007 Jun 18
Atomic structures of two novel immunoglobulin-like domain pairs in the actin cross-linking protein filamin. J Biol Chem 284(37):25450-8 (2009)
...Svergun DI, Iivanainen T, Heikkinen SM, Permi P, Koskela H, Kilpeläinen I, Ylänne J
RgGuinier 2.1 nm
Dmax 6.5 nm
VolumePorod 34 nm3

SASDAU3 – Fila22-23

immunoglobulin- like filamin two-domain fragment 22-23 experimental SAS data
GASBOR model
Sample: immunoglobulin- like filamin two-domain fragment 22-23 monomer, 19 kDa Escherichia coli protein
Buffer: 100 mM NaCl 10 mM dithiothreitol 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2007 Jun 18
Atomic structures of two novel immunoglobulin-like domain pairs in the actin cross-linking protein filamin. J Biol Chem 284(37):25450-8 (2009)
...Svergun DI, Iivanainen T, Heikkinen SM, Permi P, Koskela H, Kilpeläinen I, Ylänne J
RgGuinier 2.8 nm
Dmax 9.0 nm
VolumePorod 32 nm3

SASDAV3 – Geminin:Cdt1 2:1 heterotrimer

GemininDNA replication factor Cdt1 experimental SAS data
CRYSOL model
Sample: Geminin dimer, 47 kDa Homo sapiens protein
DNA replication factor Cdt1 monomer, 60 kDa Homo sapiens protein
Buffer: 25 mM Tris75 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2006 Aug 13
Quaternary structure of the human Cdt1-Geminin complex regulates DNA replication licensing. Proc Natl Acad Sci U S A 106(47):19807-12 (2009)
...Svergun DI, Taraviras S, Perrakis A
RgGuinier 2.9 nm
Dmax 10.0 nm
VolumePorod 70 nm3

SASDAW3 – Geminin:Cdt1 4:2 heterohexamer

GemininDNA replication factor Cdt1 experimental SAS data
CRYSOL model
Sample: Geminin dimer, 47 kDa Homo sapiens protein
DNA replication factor Cdt1 monomer, 60 kDa Homo sapiens protein
Buffer: 25 mM Tris75 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2006 Oct 5
Quaternary structure of the human Cdt1-Geminin complex regulates DNA replication licensing. Proc Natl Acad Sci U S A 106(47):19807-12 (2009)
...Svergun DI, Taraviras S, Perrakis A
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 120 nm3

SASDAX3 – MutS tetramer

DNA mismatch repair protein MutS experimental SAS data
DNA mismatch repair protein MutS Kratky plot
Sample: DNA mismatch repair protein MutS tetramer, 381 kDa Escherichia coli protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 May 12
Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation. Nucleic Acids Res 41(17):8166-81 (2013)
...Svergun DI, Friedhoff P, Sixma TK
RgGuinier 8.5 nm
Dmax 29.0 nm
VolumePorod 750 nm3

SASDAY3 – MutS tetramer

DNA mismatch repair protein MutS experimental SAS data
DNA mismatch repair protein MutS Kratky plot
Sample: DNA mismatch repair protein MutS tetramer, 381 kDa Escherichia coli protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 May 12
Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation. Nucleic Acids Res 41(17):8166-81 (2013)
...Svergun DI, Friedhoff P, Sixma TK
RgGuinier 8.3 nm
Dmax 29.0 nm
VolumePorod 720 nm3

SASDAZ3 – MutS tetramer

DNA mismatch repair protein MutS experimental SAS data
DNA mismatch repair protein MutS Kratky plot
Sample: DNA mismatch repair protein MutS tetramer, 381 kDa Escherichia coli protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 May 12
Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation. Nucleic Acids Res 41(17):8166-81 (2013)
...Svergun DI, Friedhoff P, Sixma TK
RgGuinier 8.0 nm

SASDA24 – MutS tetramer

DNA mismatch repair protein MutS experimental SAS data
DNA mismatch repair protein MutS Kratky plot
Sample: DNA mismatch repair protein MutS tetramer, 381 kDa Escherichia coli protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 May 12
Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation. Nucleic Acids Res 41(17):8166-81 (2013)
...Svergun DI, Friedhoff P, Sixma TK
RgGuinier 7.8 nm
Dmax 27.0 nm

SASDF24 – Full-length X-chromosome linked inhibitor of apoptosis protein (XIAP)

E3 ubiquitin-protein ligase XIAP experimental SAS data
HADDOCK model
Sample: E3 ubiquitin-protein ligase XIAP dimer, 113 kDa Homo sapiens protein
Buffer: Xiap buffer, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 15
Conformational characterization of full-length X-chromosome-linked inhibitor of apoptosis protein (XIAP) through an integrated approach. IUCrJ 6(Pt 5):948-957 (2019)
...Svergun DI, Banci L
RgGuinier 3.9 nm
Dmax 12.8 nm
VolumePorod 207 nm3

SASDA54 – RNA Aptamer

SRB2m experimental SAS data
DAMMIN model
Sample: SRB2m dimer, 33 kDa RNA
Buffer: Hepes, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2007 Oct 24
Characterization of a fluorophore binding RNA aptamer by fluorescence correlation spectroscopy and small angle X-ray scattering. Anal Biochem 389(1):52-62 (2009)
...Svergun DI, Hahn U
RgGuinier 2.8 nm
Dmax 10.0 nm
VolumePorod 38 nm3

SASDA74 – RNA Aptamer

SRB2m experimental SAS data
DAMMIN model
Sample: SRB2m dimer, 33 kDa RNA
Buffer: Hepes, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2007 Oct 24
Characterization of a fluorophore binding RNA aptamer by fluorescence correlation spectroscopy and small angle X-ray scattering. Anal Biochem 389(1):52-62 (2009)
...Svergun DI, Hahn U
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 31 nm3

SASDB74 – CyaA Block I-V

Adenylate cyclase toxin Block I-V experimental SAS data
DAMMIF model
Sample: Adenylate cyclase toxin Block I-V monomer, 70 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl 150 mM NaCl 10 mM CaCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Calcium-Driven Folding of RTX Domain β-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts. Mol Cell 62(1):47-62 (2016)
...Svergun DI, Barinka C, Sebo P
RgGuinier 5.6 nm
Dmax 17.2 nm
VolumePorod 275 nm3

SASDA84 – RNA Aptamer

SRB2m experimental SAS data
DAMMIN model
Sample: SRB2m dimer, 33 kDa RNA
Buffer: Hepes, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2007 Oct 24
Characterization of a fluorophore binding RNA aptamer by fluorescence correlation spectroscopy and small angle X-ray scattering. Anal Biochem 389(1):52-62 (2009)
...Svergun DI, Hahn U
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDB84 – CyaA Block V

Adenylate cyclase toxin Block V experimental SAS data
DAMMIF model
Sample: Adenylate cyclase toxin Block V monomer, 16 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl 150 mM NaCl 10 mM CaCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Calcium-Driven Folding of RTX Domain β-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts. Mol Cell 62(1):47-62 (2016)
...Svergun DI, Barinka C, Sebo P
RgGuinier 1.8 nm
Dmax 5.9 nm
VolumePorod 24 nm3

SASDA94 – Der p21

Der p21, allegren experimental SAS data
DAMMIN model
Sample: Der p21, allegren dimer, 26 kDa Escherichia coli protein
Buffer: 10 mM Hepes , pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III on 2006 Jul 24
Characterization of Der p 21, a new important allergen derived from the gut of house dust mites. Allergy 63(6):758-67 (2008)
...Svergun DI, Konarev PV, Valent P, Thalhamer J, Keller W, Valenta R, Vrtala S
RgGuinier 2.7 nm
Dmax 8.5 nm
VolumePorod 50 nm3

SASDF94 – Insulin glulisine (Apidra), oligomeric composition

Insulin glulisine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Insulin glulisine hexamer, 35 kDa protein
Buffer: Apidra formulation (per ml: 5 mg Sodium chloride, 3.15 mg m-Cresol, 6 mg Trometamol, 0.01 mg Polysorbate 20), pH: 7.3
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 20
The quaternary structure of insulin glargine and glulisine under formulation conditions. Biophys Chem 253:106226 (2019)
...Svergun D, Berchtold H
RgGuinier 2.3 nm
Dmax 7.6 nm

SASDAA4 – Full length GbpA

Full length GbpA experimental SAS data
GASBOR model
Sample: Full length GbpA monomer, 54 kDa Vibrio cholerae protein
Buffer: 25 mM Tris/HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2007 Oct 16
The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces. PLoS Pathog 8(1):e1002373 (2012)
...Svergun DI, Eijsink VG, Chatterjee NS, van Aalten DM
RgGuinier 3.9 nm
Dmax 14.5 nm
VolumePorod 100 nm3

SASDFA4 – Insulin glargine (Toujeo®), oligomeric composition

Insulin glargine (Toujeo®) experimental SAS data
PDB model
Sample: Insulin glargine (Toujeo®) hexamer, 36 kDa protein
Buffer: Toujeo Fromulation (190 ug Zinc chloride, 2.7 mg m-Cresol, 20 mg glycerol 85%), pH: 4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 5
The quaternary structure of insulin glargine and glulisine under formulation conditions. Biophys Chem 253:106226 (2019)
...Svergun D, Berchtold H
RgGuinier 1.8 nm
Dmax 6.2 nm

SASDAB4 – Truncated GbpA

Truncated GbpA experimental SAS data
GASBOR model
Sample: Truncated GbpA monomer, 44 kDa Vibrio cholerae protein
Buffer: 25 mM Tris/HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2007 Oct 16
The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces. PLoS Pathog 8(1):e1002373 (2012)
...Svergun DI, Eijsink VG, Chatterjee NS, van Aalten DM
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 80 nm3

SASDFB4 – Insulin glargine (Lantus ®), oligomeric mixture

Insulin glargine (Lantus ®) experimental SAS data
Insulin glargine (Lantus ®) Kratky plot
Sample: Insulin glargine (Lantus ®) hexamer, 36 kDa protein
Buffer: Lantus Formulation (30 µg Zinc cloride, 2.7 mg m-Cresol, 20 mg glycerol 85%), pH: 4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 23
The quaternary structure of insulin glargine and glulisine under formulation conditions. Biophys Chem 253:106226 (2019)
...Svergun D, Berchtold H
RgGuinier 1.8 nm
Dmax 5.3 nm

SASDAC4 – FilaminC 23-24

Filamin C 23-24 experimental SAS data
DAMMIN model
Sample: Filamin C 23-24 dimer, 40 kDa Escherichia coli protein
Buffer: 20 mM Tris-HCl 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2005 Jun 17
Crystal structure of human filamin C domain 23 and small angle scattering model for filamin C 23-24 dimer. J Mol Biol 368(4):1011-23 (2007)
...Svergun D, Ylänne J, Djinović Carugo K
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 75 nm3

SASDFC4 – Condensin complex subunit 3-like protein, Ycg1, extrapolated to infinite dilution

Condensin complex subunit 3-like protein experimental SAS data
DAMMIF model
Sample: Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
...Svergun DI
RgGuinier 4.6 nm
Dmax 15.6 nm
VolumePorod 236 nm3

SASDAD4 – Full length GtBP3

full length CtBP3 experimental SAS data
DAMMIN model
Sample: full length CtBP3 tetramer, 170 kDa protein
Buffer: 25 mM Tris/HCl 250 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2004 Nov 18
The C-terminal domain of the transcriptional corepressor CtBP is intrinsically unstructured. Protein Sci 15(5):1042-50 (2006)
...Svergun D, Konarev PV, Spanò S, Fasano M, Bracco C, Pesce A, Donadini A, Cericola C, Secundo F, Luini A, Corda D, Bolognesi M
RgGuinier 5.1 nm
Dmax 19.0 nm
VolumePorod 330 nm3

SASDFD4 – Condensin complex subunit 3-like protein,Ycg1, bound to condensin complex subunit 2, Brn1 (Ycg1-Brn1)

Condensin complex subunit 3-like proteinCondensin complex subunit 2 experimental SAS data
DAMMIF model
Sample: Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Condensin complex subunit 2 monomer, 17 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
...Svergun DI
RgGuinier 4.3 nm
Dmax 13.7 nm
VolumePorod 230 nm3

SASDAE4 – C-terminal CtBP3

C-term part CtBP3 experimental SAS data
DAMMIN model
Sample: C-term part CtBP3 dimer, 52 kDa protein
Buffer: 25 mM Tris/HCl 250 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2004 Nov 18
The C-terminal domain of the transcriptional corepressor CtBP is intrinsically unstructured. Protein Sci 15(5):1042-50 (2006)
...Svergun D, Konarev PV, Spanò S, Fasano M, Bracco C, Pesce A, Donadini A, Cericola C, Secundo F, Luini A, Corda D, Bolognesi M
RgGuinier 5.5 nm
Dmax 20.0 nm
VolumePorod 126 nm3

SASDFE4 – Condensin complex subunit 3-like protein, Ycg1, at 10mg/ml (tetramer)

Condensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein tetramer, 433 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
...Svergun DI
RgGuinier 8.3 nm
Dmax 32.7 nm
VolumePorod 1080 nm3

SASDAF4 – DmMfe2

Peroxisomal multifunctional enzyme type 2  experimental SAS data
Peroxisomal multifunctional enzyme type 2  Kratky plot
Sample: Peroxisomal multifunctional enzyme type 2 dimer, 128 kDa Drosophila melanogaster protein
Buffer: 20 mM Sodium Phosphate 200 mM NaCl 5% (v/v) Glycerol 1mM Na2EDTA 1 mM NaN3, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Jun 12
Quaternary structure of human, Drosophila melanogaster and Caenorhabditis elegans MFE-2 in solution from synchrotron small-angle X-ray scattering. FEBS Lett 587(4):305-10 (2013)
...Svergun DI, Glumoff T
RgGuinier 3.6 nm
Dmax 12.0 nm

SASDBF4 – Wild-type LytA (N-His6) with choline

Lytic Amidase with choline experimental SAS data
SASREF model
Sample: Lytic Amidase with choline dimer, 75 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 Jul 2
Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
...Svergun D, Henriques-Normark B, Achour A
RgGuinier 4.9 nm
Dmax 15.0 nm
VolumePorod 115 nm3

SASDFF4 – Condensin complex subunit 3-like protein, Ycg1, at 5mg/ml

Condensin complex subunit 3-like proteinCondensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein tetramer, 433 kDa Chaetomium thermophilum protein
Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
...Svergun DI
RgGuinier 6.8 nm
Dmax 29.8 nm
VolumePorod 720 nm3

SASDJF4 – Synthetic nanobody Sybody 23 (Sy23)

Synthetic nanobody Sybody 23 experimental SAS data
CORAL model
Sample: Synthetic nanobody Sybody 23 monomer, 16 kDa synthetic construct protein
Buffer: 50 mM Tris 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 May 5
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 (2020)
...Svergun D, Dobrev N, Remans K, Seeger M, McInerney G, Murrell B, Hällberg B, Löw C
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 22 nm3

SASDAG4 – HsMfe2

Peroxisomal multifunctional enzyme type 2 experimental SAS data
Peroxisomal multifunctional enzyme type 2 Kratky plot
Sample: Peroxisomal multifunctional enzyme type 2 dimer, 159 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 200 mM NaCl 5% (v/v) Glycerol 1mM Na2EDTA 1 mM NaN3, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 Mar 22
Quaternary structure of human, Drosophila melanogaster and Caenorhabditis elegans MFE-2 in solution from synchrotron small-angle X-ray scattering. FEBS Lett 587(4):305-10 (2013)
...Svergun DI, Glumoff T
RgGuinier 4.6 nm
Dmax 15.0 nm

SASDBG4 – Prophage LytA with choline

Prophage Lytic Amidase with choline experimental SAS data
SASREF model
Sample: Prophage Lytic Amidase with choline dimer, 73 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 Jul 2
Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
...Svergun D, Henriques-Normark B, Achour A
RgGuinier 5.9 nm
Dmax 17.0 nm
VolumePorod 130 nm3