Chromochloris zofingiensis Hexokinase-1 (HXK-1)

Estella Yee

Submitted to SASBDB: 2024 Apr 29 Published in SASBDB: 2026 Apr 29

SASDU38 – Chromochloris zofingiensis Hexokinase-1 (37-499) wild-type Sample: Chromochloris zofingiensis Hexokinase-1 monomer, 55 kDa Chromochloris zofingiensis protein Buffer: 50 mM HEPES, 150 mM NaCl, 10 mM MgCl2, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Mar 25 RgGuinier 2.6 nm Dmax 7.9 nm VolumePorod 72 nm3

SASDU48 – Chromochloris zofingiensis Hexokinase-1 (37-499) wild-type with 200 mM glucose Sample: Chromochloris zofingiensis Hexokinase-1 monomer, 55 kDa Chromochloris zofingiensis protein Buffer: 50 mM HEPES, 150 mM NaCl, 10 mM MgCl2, 1 mM TCEP, 200 mM glucose, pH: 8 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Mar 22 RgGuinier 2.5 nm Dmax 7.6 nm VolumePorod 74 nm3

SASDU58 – Chromochloris zofingiensis Hexokinase-1 (37-499) E291W variant with 200 mM glucose Sample: Chromochloris zofingiensis Hexokinase-1 (E291W) monomer, 55 kDa Chromochloris zofingiensis protein Buffer: 50 mM HEPES, 150 mM NaCl, 10 mM MgCl2, 1 mM TCEP, 200 mM glucose, pH: 8 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Mar 22 RgGuinier 2.7 nm Dmax 8.6 nm VolumePorod 87 nm3

SASDU68 – Chromochloris zofingiensis Hexokinase-1 (37-499) E291W variant Sample: Chromochloris zofingiensis Hexokinase-1 (E291W) monomer, 55 kDa Chromochloris zofingiensis protein Buffer: 50 mM HEPES, 150 mM NaCl, 10 mM MgCl2, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Feb 10 RgGuinier 2.6 nm Dmax 8.3 nm VolumePorod 75 nm3