The Paulinella chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold

Klimenko V, Reiners J, Applegate V, Reimann K, Popowicz G, Hoeppner A, Papadopoulos A, Smits S, Nowack E
Plant Physiology (2025 Oct 10)

doi: 10.1093/plphys/kiaf504

Submitted to SASBDB: 2024 Dec 4 Published in SASBDB: 2025 Oct 13

SASDWS3 – N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) Sample: N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) dimer, 97 kDa Paulinella chromatophora protein Buffer: 20 mM HEPES, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2023 Mar 10 RgGuinier 3.6 nm Dmax 13.1 nm VolumePorod 170 nm3

SASDWT3 – crTPpart2 of N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) Sample: CrTPpart2 of N-acetyl-gamma-glutamyl-phosphate reductase (ArgC) monomer, 14 kDa Paulinella chromatophora protein Buffer: 20 mM HEPES, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14 RgGuinier 1.7 nm Dmax 5.8 nm VolumePorod 27 nm3

SASDWU3 – RNA helicase (RnaH) Sample: RNA helicase (RnaH) monomer, 63 kDa Paulinella chromatophora protein Buffer: 20 mM HEPES, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2024 Oct 28 RgGuinier 3.6 nm Dmax 13.7 nm VolumePorod 132 nm3

SASDWV3 – crTPpart2 of RNA helicase (RnaH) Sample: CrTPpart2 of RNA helicase (RnaH) monomer, 21 kDa Paulinella chromatophora protein Buffer: 20 mM HEPES, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 15 RgGuinier 2.3 nm Dmax 9.2 nm VolumePorod 46 nm3

SASDWW3 – crTPpart2 of cysteine synthase A (CysK) Sample: CrTPpart2 of cysteine synthase A (CysK) monomer, 16 kDa Paulinella chromatophora protein Buffer: 20 mM HEPES, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14 RgGuinier 1.7 nm Dmax 6.2 nm VolumePorod 28 nm3