Dynamic RNA stem-loops Drive dimerization-assisted higher-order assemblies of SARS-CoV-2 Nsp9

Jill Trewhella

Submitted to SASBDB: 2026 Feb 6 Published in SASBDB: 2026 Oct 9

SASDYQ6 – SARS-CoV-2 Non-structural protein 9 (Nsp9) at 6 mg/mL Sample: SARS-CoV-2 non-structural protein 9 (Nsp9) dimer, 25 kDa Severe acute respiratory … protein Buffer: 25 mM NaPO4, 150 mM NaCl, 1 mM DTT with 5% glycerol, pH: 6 Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2025 Nov 13 RgGuinier 2.1 nm Dmax 8.1 nm VolumePorod 36 nm3

SASDYR6 – SARS-CoV-2 non-structural protein 9 (Nsp9) with the synthetic RNA 4.5Stet Sample: Synthetic RNA 4.5Stet, 5 kDa RNA SARS-CoV-2 non-structural protein 9 (Nsp9) dimer, 25 kDa Severe acute respiratory … protein Buffer: 25 mM NaPO4 pH 6.0, 150 mM NaCl, 1 mM DTT with 5% glycerol, pH: 6 Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2025 Nov 13 RgGuinier 2.1 nm Dmax 8.5 nm VolumePorod 40 nm3

SASDYS6 – SARS-CoV-2 non-structural protein 9 (Nsp9) with the modified synthetic RNA dSSO(PS-m) Sample: SARS-CoV-2 non-structural protein 9 (Nsp9) dimer, 25 kDa Severe acute respiratory … protein Modified synthetic RNA dSSO(PS-m) monomer, 6 kDa RNA Buffer: 25 mM NaPO4, 150 mM NaCl, 1 mM DTT with 5% glycerol, pH: 6 Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2025 Nov 13 RgGuinier 2.9 nm Dmax 12.5 nm VolumePorod 52 nm3