Small Angle Scattering Biological Data Bank SASBDB


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23 hits found for Jill Trewhella

SASDCK2 – Glucose Isomerase - Streptomyces rubiginosus

Sample:	Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, ...

R_g^Guinier	3.3	nm
D_max	9.2	nm
Volume^Porod	229	nm³

SASDCQ2 – 4Ca2+-calmodulin - Xenopus laevis

4Ca2+-calmodulin - Xenopus laevis Rg histogram

Sample:	Calmodulin-1 monomer, 17 kDa Xenopus laevis protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

R_g^Guinier	2.2	nm
D_max	7.2	nm
Volume^Porod	25	nm³

SASDCA3 – Zinc finger and BTB domain-containing protein 38 (ZBTB38) complexed with methylated C-terminal ZBTB38 binding sequence

Zinc finger and BTB domain-containing protein 38methylated C-terminal ZBTB38 binding sequence experimental SAS data

Sample:	Zinc finger and BTB domain-containing protein 38 monomer, 17 kDa Homo sapiens protein methylated C-terminal ZBTB38 binding sequence monomer, 17 kDa DNA
Buffer:	10 mM Tris, 1 mM tris(2-carboxy-ethyl)phosphine (TCEP), 0.05% NaN3, 10% D2O, pH: 6.8
Experiment:	SAXS data collected at Anton Paar SAXSess, Department of Chemistry, University of Utah on 2016 Nov 11

The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. J Mol Biol 430(3):258-271 (2018)
...Trewhella J, Terooatea TW, Miller SA, Buck-Koehntop BA

R_g^Guinier	2.5	nm
D_max	8.2	nm
Volume^Porod	50	nm³

SASDCB3 – Methylated C-terminal ZBTB38 binding sequence (mCZ38BS)

methylated C-terminal ZBTB38 binding sequence experimental SAS data

Sample:	methylated C-terminal ZBTB38 binding sequence monomer, 17 kDa DNA
Buffer:	10 mM Tris, 1 mM tris(2-carboxy-ethyl)phosphine (TCEP), 0.05% NaN3, 10% D2O, pH: 6.8
Experiment:	SAXS data collected at Anton Paar SAXSess, Department of Chemistry, University of Utah on 2016 Oct 27

The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. J Mol Biol 430(3):258-271 (2018)
...Trewhella J, Terooatea TW, Miller SA, Buck-Koehntop BA

R_g^Guinier	2.5	nm
D_max	9.2	nm
Volume^Porod	23	nm³

SASDCJ3 – Bovine Serum Albumin

Sample:	Serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

R_g^Guinier	2.8	nm
D_max	8.7	nm
Volume^Porod	101	nm³

SASDKR3 – Deuterated calmodulin bound to the HIV-1 MA protein

Calmodulin-1calcium ionsGag-Pol polyprotein experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Xenopus laevis protein calcium ions tetramer, 0 kDa Gag-Pol polyprotein monomer, 15 kDa Human immunodeficiency virus … protein
Buffer:	50 mM MOPS, 5 mM CaCl2, 2 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at Bruker Nanostar, Australian Nuclear Science and Technology Organisation on 2010 Jun 23

Calmodulin binds a highly extended HIV-1 MA protein that refolds upon its release. Biophys J 103(3):541-549 (2012)
...Trewhella J

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	55	nm³

SASDPP4 – Consensus SAXS Profile - Ribonuclease A

Ribonuclease pancreatic experimental SAS data

Sample:	Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...

R_g^Guinier	1.5	nm
D_max	4.9	nm
Volume^Porod	18	nm³

SASDPQ4 – Consensus SAXS Profile - Urate Oxidase

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.2	nm
D_max	9.2	nm
Volume^Porod	220	nm³

SASDPR4 – Consensus SAXS Profile - Xylose Isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	ConsensusBuffer_50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.3	nm
D_max	10.1	nm
Volume^Porod	243	nm³

SASDPS4 – Consensus SAXS Profile - Xylanase

Endo-1,4-beta-xylanase experimental SAS data

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.6	nm
D_max	5.1	nm
Volume^Porod	27	nm³

SASDPT4 – Consensus SAXS Profile - Lysozyme

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.5	nm
D_max	4.8	nm
Volume^Porod	19	nm³

SASDPU4 – Consensus SANS Profile - Ribonuclease A in 100% v/v D2O buffer

Sample:	Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.4	nm
D_max	4.4	nm

SASDPV4 – Consensus SANS Profile - Lysozyme in 100% v/v D2O buffer

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.2	nm
D_max	3.8	nm

SASDPW4 – Consensus SANS Profile - Xylanase in 100% v/v D2O buffer

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.5	nm
D_max	4.4	nm

SASDPX4 – Consensus SANS Profile - Urate oxidase in 100% v/v D2O buffer

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.1	nm
D_max	9.3	nm

SASDPY4 – Consensus SANS Profile - Xylose isomerase in 100% v/v D2O buffer

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.1	nm
D_max	9.5	nm

SASDPZ4 – Consensus SANS Profile - Lysozyme in H2O buffer

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22

R_g^Guinier	1.4	nm
D_max	4.8	nm

SASDB25 – Cardiac myosin binding protein-C: domains C5-C6-C7

Cardiac myosin binding protein-C: domains C5-C6-C7 experimental SAS data

Cardiac myosin binding protein-C: domains C5-C6-C7 Kratky plot

Sample:	Cardiac myosin binding protein-C: domains C5-C6-C7 monomer, 36 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 250 mM NaCl, 2 mM TCEP, 0.02% sodium azide, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 18

Clinically Linked Mutations in the Central Domains of Cardiac Myosin-Binding Protein C with Distinct Phenotypes Show Differential Structural Effects. Structure 24(1):105-115 (2016)
...Trewhella J

R_g^Guinier	3.8	nm
D_max	14.1	nm
Volume^Porod	55	nm³

SASDP25 – Consensus SANS Profile - Ribonuclease A in H2O buffer

Sample:	Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22

R_g^Guinier	1.5	nm
D_max	4.1	nm

SASDP35 – Consensus SANS Profile - Xylanase in H2O buffer

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22

R_g^Guinier	1.6	nm
D_max	4.3	nm

SASDP45 – Consensus SANS Profile - Urate oxidase in H2O buffer

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24

R_g^Guinier	3.2	nm
D_max	9.1	nm

SASDP55 – Consensus SANS Profile - Xylose isomerase in H2O buffer

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24

R_g^Guinier	3.3	nm
D_max	9.7	nm

SASDDD9 – Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain

cardiac myosin binding protein C: tri-helix bundle-C2 experimental SAS data

Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain Rg histogram

Sample:	cardiac myosin binding protein C: tri-helix bundle-C2 monomer, 15 kDa human sequence obtained … protein
Buffer:	150 mM NaCl, 10 mM MES, 2 mM TCEP, 1 mM NaN3 at 4°C, pH: 6.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 18

A Highly Conserved Yet Flexible Linker Is Part of a Polymorphic Protein-Binding Domain in Myosin-Binding Protein C. Structure 24(11):2000-2007 (2016)
...Trewhella J

R_g^Guinier	1.9	nm
D_max	8.0	nm
Volume^Porod	20	nm³

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