Use of biomolecular emulator for characterizing flexible proteins by small-angle x-ray scattering.

Haitin Y, Manori B, Giladi M
Protein Sci 35(6):e70646 (2026 Jun)

PMID: 42178622 doi: 10.1002/pro.70646

Submitted to SASBDB: 2026 Mar 6 Published in SASBDB: 2026 Aug 27

SASDZB3 – Calcium binding domains 1 and 2 from sodium-calcium exchanger 1.4, apo state Sample: Calcium Binding Domains 1 and 2 from Sodium-Calcium Exchanger 1.4 monomer, 32 kDa Canis lupus familiaris protein Buffer: 20 mM Tris-HCl, 100 mM KCl, 20 mM -mercaptoethanol, 10 mM EDTA, pH: 7.2 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Apr 30 RgGuinier 2.9 nm

SASDZC3 – Calcium binding domains 1 and 2 from sodium-calcium exchanger 1.4, calcium-bound state Sample: Calcium Binding Domains 1 and 2 from Sodium-Calcium Exchanger 1.4 monomer, 32 kDa Canis lupus familiaris protein Buffer: 20 mM Tris-HCl, 100 mM KCl, 20 mM -mercaptoethanol, 10 mM CaCl2, pH: 7.2 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Apr 30 RgGuinier 3.2 nm

SASDZD3 – Double C2-like domain-containing protein beta (DOC2B) C2AB domains from , apo state Sample: Double C2-like domain-containing protein beta monomer, 33 kDa Rattus norvegicus protein Buffer: 20 mM Tris–HCl, 200 mM NaCl, 20 mM β-mercaptoethanol, 10 mM EDTA, pH: 6.8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Apr 30 RgGuinier 2.6 nm

SASDZE3 – Chloride intracellular channel protein 5 Sample: Chloride intracellular channel protein 5 monomer, 27 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 10 mM beta-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2012 Apr 30 RgGuinier 2.2 nm

SASDZF3 – Chloride intracellular channel protein 5, F34D mutant Sample: Chloride intracellular channel protein 5 monomer, 27 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 10 mM beta-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2023 Jul 13 RgGuinier 2.1 nm