Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle.

Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
Nature 568(7753):571-575 (2019 Apr)

PMID: 30944476 doi: 10.1038/s41586-019-1095-5

Submitted to SASBDB: 2018 Oct 10 Published in SASBDB: 2019 Apr 2

SASDFA3 – Human ATP-citrate synthase (ACLY) full length in HBS Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4 RgGuinier 6.1 nm Dmax 19.0 nm VolumePorod 765 nm3

SASDFB3 – Human ATP-citrate synthers (ACLY) full length in HBS + Citrate Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4 RgGuinier 6.2 nm Dmax 19.0 nm VolumePorod 787 nm3

SASDFC3 – Human ATP-citrate synthase (ACLY) full length in HBS + Citrate + Coenzyme-A Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4 RgGuinier 5.9 nm Dmax 17.0 nm VolumePorod 775 nm3

SASDE36 – Human ATP-citrate synthase (ACLY) in HBS Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 6 RgGuinier 6.0 nm Dmax 17.5 nm VolumePorod 738 nm3

SASDE46 – Human ATP-citrate synthers (ACLY) in HBS + Citrate Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 6 RgGuinier 6.1 nm Dmax 17.5 nm VolumePorod 747 nm3

SASDE56 – Human ATP-citrate synthase (ACLY) in HBS + Citrate + Coenzyme-A Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 5 RgGuinier 5.8 nm Dmax 16.5 nm VolumePorod 709 nm3

SASDE66 – C. limicola ATP-citrate lyase (ACL) in HBS Sample: ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4 RgGuinier 6.1 nm Dmax 20.0 nm VolumePorod 666 nm3

SASDE76 – C. limicola ATP-citrate lyase (ACL) in HBS + Citrate Sample: ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4 RgGuinier 6.0 nm Dmax 20.0 nm VolumePorod 672 nm3

SASDE86 – C. limicola ATP-citrate lyase (ACL) in HBS + Citrate + Coenzyme-A Sample: ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4 RgGuinier 5.7 nm Dmax 17.5 nm VolumePorod 791 nm3