Structure and dynamics of UBA5-UFM1 complex formation showing new insights in the UBA5 activation mechanism

Fuchs S, Kikhney A, Schubert R, Kaiser C, Liebau E, Svergun D, Betzel C, Perbandt M
Journal of Structural Biology :107796 (2021 Sep)

doi: 10.1016/j.jsb.2021.107796

Submitted to SASBDB: 2021 Apr 11 Published in SASBDB: 2021 Jul 13

SASDL73 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 2 mg/mL + UFM1 0.5 mg/mL) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29 RgGuinier 2.9 nm Dmax 11.0 nm

SASDL83 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 3 mg/mL + UFM1 0.75 mg/ml) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29 RgGuinier 3.0 nm Dmax 12.0 nm

SASDL93 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 4 mg/mL + UFM1 1.0 mg/ml) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29 RgGuinier 3.1 nm Dmax 13.0 nm

SASDLA3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 5 mg/mL + UFM1 1.25 mg/ml) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29 RgGuinier 3.1 nm Dmax 13.0 nm VolumePorod 100 nm3

SASDLB3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 6 mg/mL + UFM1 1.5 mg/mL) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29 RgGuinier 3.1 nm Dmax 13.0 nm VolumePorod 103 nm3

SASDLC3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 7 mg/mL + UFM1 1.75 mg/mL) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29 RgGuinier 3.1 nm Dmax 13.0 nm VolumePorod 104 nm3

SASDLD3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 8 mg/mL + UFM1 2 mg/mL) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29 RgGuinier 3.1 nm Dmax 13.0 nm VolumePorod 106 nm3

SASDLE3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 9 mg/mL + UFM1 2.25 mg/mL) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29 RgGuinier 3.2 nm Dmax 14.5 nm VolumePorod 111 nm3

SASDGG6 – Ubiquitin activating enzyme 5 (0.3 mg/ml) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13 RgGuinier 2.8 nm Dmax 9.0 nm VolumePorod 74 nm3

SASDGH6 – Ubiquitin activating enzyme 5 (1.0 mg/ml) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13 RgGuinier 2.9 nm Dmax 10.0 nm VolumePorod 82 nm3

SASDGM6 – Ubiquitin activating enzyme 5 (1.9 mg/ml) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13 RgGuinier 2.9 nm Dmax 9.8 nm VolumePorod 94 nm3

SASDGL6 – Ubiquitin activating enzyme 5 (3.6 mg/ml) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13 RgGuinier 3.0 nm Dmax 11.0 nm VolumePorod 97 nm3

SASDGK6 – Ubiquitin activating enzyme 5 (4.4 mg/ml) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13 RgGuinier 3.1 nm Dmax 12.0 nm VolumePorod 104 nm3

SASDGJ6 – Ubiquitin activating enzyme 5 (6.8 mg/ml) Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13 RgGuinier 3.2 nm Dmax 12.0 nm VolumePorod 108 nm3

SASDM82 – Ubiquitin-fold modifier 1 Sample: Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13 RgGuinier 1.5 nm Dmax 5.1 nm VolumePorod 16 nm3