Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES.

Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA
Nucleic Acids Res (2020 Jun 18)

PMID: 32556302 doi: 10.1093/nar/gkaa519

Submitted to SASBDB: 2020 Jan 21 Published in SASBDB: 2020 Jun 23

SASDH55 – Modified RNA stem loop IV poliovirus IRES (SLIVm) Sample: Modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Dec 2 RgGuinier 3.5 nm Dmax 12.0 nm VolumePorod 94 nm3

SASDH65 – Poly(rC)-binding protein 2 (PCBP2) Sample: Poly(rC)-binding protein 2 monomer, 40 kDa Homo sapiens protein Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jul 16 RgGuinier 3.3 nm Dmax 13.2 nm VolumePorod 76 nm3

SASDH75 – Poly(rC)-binding protein 2 (PCBP2) with bound modified RNA stem loop IV poliovirus IRES (SLIVm) - PCBP2/SLIVm complex Sample: Poly(rC)-binding protein 2 monomer, 40 kDa Homo sapiens protein Modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Dec 16 RgGuinier 3.7 nm Dmax 11.5 nm VolumePorod 162 nm3

SASDH85 – Truncated poly(rC)-binding protein 2 (PCBP2-ΔKH3) with bound modified RNA stem loop IV poliovirus IRES (SLIVm) Sample: Modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA Truncated poly(rC)-binding protein 2 (ΔKH3) monomer, 28 kDa Homo sapiens protein Truncated poly(rC)-binding protein 2 (ΔKH3) monomer, 28 kDa Homo sapiens protein Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jul 1 RgGuinier 3.8 nm Dmax 12.2 nm VolumePorod 165 nm3

SASDH95 – Truncated Poly(rC)-binding protein 2 (PCBP2-ΔKH3) Sample: Truncated poly(rC)-binding protein 2 (ΔKH3) monomer, 28 kDa Homo sapiens protein Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jan 1 RgGuinier 2.6 nm Dmax 9.2 nm VolumePorod 66 nm3

SASDHA5 – Truncated poly(rC)-binding protein 2 (PCBP2-ΔKH1-KH2) with bound modified RNA stem loop IV poliovirus IRES (SLIVm) Sample: Modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA Truncated poly(rC)-binding protein 2 (ΔKH1-KH2) monomer, 18 kDa Homo sapiens protein Buffer: 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jul 1 RgGuinier 3.5 nm Dmax 12.2 nm VolumePorod 126 nm3