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44 hits found for Adriana Erica Miele

SASDN24 – Heparin oligosaccharide DP10

Heparine oligosaccharide of 10 sugar units (5 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 10 sugar units (5 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 10 sugar units (5 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 3 kDa Sus scrofa domesticus
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 May 6
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.3 nm
Dmax 5.7 nm

SASDN34 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, pH 7.4

Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.5 nm
Dmax 8.5 nm

SASDN44 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4

Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.5 nm
Dmax 6.8 nm

SASDN54 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4

Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.4 nm
Dmax 8.6 nm

SASDN64 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4

Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.5 nm
Dmax 6.8 nm

SASDN74 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.8 nm
Dmax 6.3 nm

SASDN84 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.8 nm
Dmax 7.7 nm

SASDN94 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.8 nm
Dmax 7.9 nm

SASDNA4 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.8 nm
Dmax 7.5 nm

SASDNB4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 1.25 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 4.8 nm
Dmax 29.7 nm

SASDNC4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 2.5 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 4.8 nm
Dmax 23.9 nm

SASDND4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 5mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.7 nm
Dmax 23.7 nm

SASDNE4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 10 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 2.4 nm
Dmax 20.8 nm

SASDNF4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 20 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.4 nm
Dmax 22.2 nm

SASDNG4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 40 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.0 nm
Dmax 22.1 nm

SASDNH4 – Heparin oligosaccharide in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 4.6 nm
Dmax 23.9 nm

SASDNJ4 – Heparin oligosaccharide in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 5.1 nm
Dmax 27.5 nm

SASDNK4 – Heparin oligosaccharide in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 5.1 nm
Dmax 30.8 nm

SASDNL4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl pH 7.4, 1.25 mg/mL

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.2 nm
Dmax 29.6 nm

SASDNM4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl pH 7.4, 2.5 mg/mL

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.2 nm
Dmax 26.2 nm

SASDNN4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl pH 7.4, 5 mg/mL

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.1 nm
Dmax 19.8 nm

SASDNP4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.9 nm
Dmax 22.2 nm

SASDNQ4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.8 nm
Dmax 22.7 nm

SASDNR4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.8 nm
Dmax 20.6 nm

SASDJ46 – Fep1-rec-GATA

GATA-type iron responsive transcription factor Fep1 reconstituted24-mer double strand DNA from the GATA promoter experimental SAS data
GATA-type iron responsive transcription factor Fep1 reconstituted 24-mer double strand DNA from the GATA promoter Kratky plot
Sample: GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein
24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.5 nm
Dmax 13.1 nm
VolumePorod 73 nm3

SASDJ56 – Fep1-GATA

GATA-type iron responsive transcription factor Fep124-mer double strand DNA from the GATA promoter experimental SAS data
GATA-type iron responsive transcription factor Fep1 24-mer double strand DNA from the GATA promoter Kratky plot
Sample: GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein
24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 85 nm3

SASDJ66 – GATA double strand DNA

24-mer double strand DNA from the GATA promoter experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 10 nm3

SASDJ76 – Fep1 wild type with [2Fe2S] cluster reconstituted

GATA-type iron responsive transcription factor Fep1 reconstituted experimental SAS data
Fep1 wild type with [2Fe2S] cluster reconstituted Rg histogram
Sample: GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.8 nm
Dmax 16.5 nm
VolumePorod 53 nm3

SASDJ86 – Fep1 wild type

GATA-type iron responsive transcription factor Fep1 experimental SAS data
Fep1 wild type Rg histogram
Sample: GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.5 nm
Dmax 11.8 nm
VolumePorod 41 nm3

SASDZA8 – Polyclonal antibody anti-SmVAL11

Polyclonal antibody anti-SmVAL11 experimental SAS data
Polyclonal antibody anti-SmVAL11 Kratky plot
Sample: Polyclonal antibody anti-SmVAL11 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) in complex with cognate polyclonal antibody (pAb anti-SmVAL11)
Adriana Erica Miele
RgGuinier 4.7 nm
Dmax 16.4 nm
VolumePorod 193 nm3

SASDZB8 – Schistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) in complex with pAb anti-SmVAL11, ratio 2:1 (2nd peak)

Venom Allergen-like protein 11Polyclonal antibody anti-SmVAL11 experimental SAS data
DAMMIF model
Sample: Venom Allergen-like protein 11 , 47 kDa Schistosoma mansoni protein
Polyclonal antibody anti-SmVAL11 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) in complex with cognate polyclonal antibody (pAb anti-SmVAL11)
Adriana Erica Miele
RgGuinier 4.9 nm
Dmax 17.2 nm
VolumePorod 206 nm3

SASDZC8 – Schistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) in complex with pAb anti-SmVAL11, ratio 2:1 (1st peak)

Venom Allergen-like protein 11Polyclonal antibody anti-SmVAL11 experimental SAS data
Venom Allergen-like protein 11 Polyclonal antibody anti-SmVAL11 Kratky plot
Sample: Venom Allergen-like protein 11 , 47 kDa Schistosoma mansoni protein
Polyclonal antibody anti-SmVAL11 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) in complex with cognate polyclonal antibody (pAb anti-SmVAL11)
Adriana Erica Miele
RgGuinier 7.9 nm
Dmax 33.2 nm
VolumePorod 688 nm3

SASDZD8 – Schistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) in complex with pAb anti-SmVAL11, ratio 1:1 (2nd peak)

Venom Allergen-like protein 11Polyclonal antibody anti-SmVAL11 experimental SAS data
Venom Allergen-like protein 11 Polyclonal antibody anti-SmVAL11 Kratky plot
Sample: Venom Allergen-like protein 11 , 47 kDa Schistosoma mansoni protein
Polyclonal antibody anti-SmVAL11 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) in complex with cognate polyclonal antibody (pAb anti-SmVAL11)
Adriana Erica Miele
RgGuinier 4.9 nm
Dmax 16.4 nm
VolumePorod 204 nm3

SASDZE8 – Schistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) in complex with pAb anti-SmVAL11, ratio 1:1 (1st peak)

Venom Allergen-like protein 11Polyclonal antibody anti-SmVAL11 experimental SAS data
Venom Allergen-like protein 11 Polyclonal antibody anti-SmVAL11 Kratky plot
Sample: Venom Allergen-like protein 11 , 47 kDa Schistosoma mansoni protein
Polyclonal antibody anti-SmVAL11 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) in complex with cognate polyclonal antibody (pAb anti-SmVAL11)
Adriana Erica Miele
RgGuinier 8.2 nm
Dmax 35.6 nm
VolumePorod 640 nm3

SASDZF8 – Schistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) in complex with pAb anti-SmVAL11, ratio 0.5:1 (2nd peak)

Venom Allergen-like protein 11Polyclonal antibody anti-SmVAL11 experimental SAS data
Venom Allergen-like protein 11 Polyclonal antibody anti-SmVAL11 Kratky plot
Sample: Venom Allergen-like protein 11 , 47 kDa Schistosoma mansoni protein
Polyclonal antibody anti-SmVAL11 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) in complex with cognate polyclonal antibody (pAb anti-SmVAL11)
Adriana Erica Miele
RgGuinier 4.8 nm
Dmax 16.1 nm
VolumePorod 194 nm3

SASDZG8 – Schistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) in complex with pAb anti-SmVAL11, ratio 0.5:1 (1st peak)

Venom Allergen-like protein 11Polyclonal antibody anti-SmVAL11 experimental SAS data
Venom Allergen-like protein 11 Polyclonal antibody anti-SmVAL11 Kratky plot
Sample: Venom Allergen-like protein 11 , 47 kDa Schistosoma mansoni protein
Polyclonal antibody anti-SmVAL11 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) in complex with cognate polyclonal antibody (pAb anti-SmVAL11)
Adriana Erica Miele
RgGuinier 8.6 nm
Dmax 42.7 nm
VolumePorod 573 nm3

SASDZJ8 – Schistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) - Cleaved domains

Venom Allergen-like protein 11 experimental SAS data
Sample: Venom Allergen-like protein 11 , 47 kDa Schistosoma mansoni protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2024 Jul 11
Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) in complex with cognate polyclonal antibody (pAb anti-SmVAL11)
Adriana Erica Miele
RgGuinier 2.2 nm
Dmax 7.0 nm
VolumePorod 20 nm3

SASDZK8 – Schistosoma mansoni Venom Allergen-like protein isoform 13, alone

Venom allergen-like protein 13 experimental SAS data
Sample: Venom allergen-like protein 13 monomer, 27 kDa Schistosoma mansoni protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2024 Apr 17
Schistosoma mansoni Venom Allergen-like protein 13 (SmVAL13) in complex with cognate polyclonal antibody (pAb anti-SmVAL13)
Adriana Erica Miele
RgGuinier 2.5 nm
Dmax 15.1 nm
VolumePorod 32 nm3

SASDZL8 – Schistosoma mansoni Venom Allergen-like protein isoform 13 (SmVAL13) in complex with pAb anti-SmVAL13 in ratio 2:1 (2nd peak)

Venom allergen-like protein 13Polyclonal antibody anti-SmVAL13 experimental SAS data
DAMMIF model
Sample: Venom allergen-like protein 13 monomer, 27 kDa Schistosoma mansoni protein
Polyclonal antibody anti-SmVAL13 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 13 (SmVAL13) in complex with cognate polyclonal antibody (pAb anti-SmVAL13)
Adriana Erica Miele
RgGuinier 4.7 nm
Dmax 16.0 nm
VolumePorod 185 nm3

SASDZM8 – Schistosoma mansoni Venom Allergen-like protein isoform 13 (SmVAL13) in complex with pAb anti-SmVAL13 in ratio 2:1 (1st peak)

Venom allergen-like protein 13Polyclonal antibody anti-SmVAL13 experimental SAS data
Venom allergen-like protein 13 Polyclonal antibody anti-SmVAL13 Kratky plot
Sample: Venom allergen-like protein 13 monomer, 27 kDa Schistosoma mansoni protein
Polyclonal antibody anti-SmVAL13 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 13 (SmVAL13) in complex with cognate polyclonal antibody (pAb anti-SmVAL13)
Adriana Erica Miele
RgGuinier 9.0 nm
Dmax 36.9 nm
VolumePorod 955 nm3

SASDZN8 – Schistosoma mansoni Venom Allergen-like protein isoform 13 (SmVAL13 ) in complex with pAb anti-SmVAL13 in ratio 1:1 (1st peak)

Venom allergen-like protein 13Polyclonal antibody anti-SmVAL13 experimental SAS data
Venom allergen-like protein 13 Polyclonal antibody anti-SmVAL13 Kratky plot
Sample: Venom allergen-like protein 13 monomer, 27 kDa Schistosoma mansoni protein
Polyclonal antibody anti-SmVAL13 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 13 (SmVAL13) in complex with cognate polyclonal antibody (pAb anti-SmVAL13)
Adriana Erica Miele
RgGuinier 8.6 nm
Dmax 32.4 nm
VolumePorod 760 nm3

SASDZP8 – Schistosoma mansoni Venom Allergen-like protein isoform 13 (SmVAL13) in complex with pAb anti-SmVAL13 in ratio 1:1 (2nd peak)

Venom allergen-like protein 13Polyclonal antibody anti-SmVAL13 experimental SAS data
Venom allergen-like protein 13 Polyclonal antibody anti-SmVAL13 Kratky plot
Sample: Venom allergen-like protein 13 monomer, 27 kDa Schistosoma mansoni protein
Polyclonal antibody anti-SmVAL13 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 13 (SmVAL13) in complex with cognate polyclonal antibody (pAb anti-SmVAL13)
Adriana Erica Miele
RgGuinier 4.4 nm
Dmax 14.7 nm
VolumePorod 159 nm3

SASDZQ8 – Schistosoma mansoni Venom Allergen-like protein isoform 13 (SmVAL13) in complex with pAb anti-SmVAL13 in ratio 0.5:1 (2nd peak)

Venom allergen-like protein 13Polyclonal antibody anti-SmVAL13 experimental SAS data
Venom allergen-like protein 13 Polyclonal antibody anti-SmVAL13 Kratky plot
Sample: Venom allergen-like protein 13 monomer, 27 kDa Schistosoma mansoni protein
Polyclonal antibody anti-SmVAL13 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 13 (SmVAL13) in complex with cognate polyclonal antibody (pAb anti-SmVAL13)
Adriana Erica Miele
RgGuinier 4.6 nm
Dmax 14.4 nm
VolumePorod 163 nm3

SASDZR8 – Polyclonal antibody anti-SmVAL13

Polyclonal antibody anti-SmVAL13 experimental SAS data
DAMMIF model
Sample: Polyclonal antibody anti-SmVAL13 monomer, 140 kDa Lagomorph protein
Buffer: 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH: 6.8
Experiment: SAXS data collected at SWING, SOLEIL on 2025 Jan 22
Schistosoma mansoni Venom Allergen-like protein 13 (SmVAL13) in complex with cognate polyclonal antibody (pAb anti-SmVAL13)
Adriana Erica Miele
RgGuinier 5.2 nm
Dmax 16.2 nm
VolumePorod 214 nm3