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29 hits found for Adriana Erica Miele

SASDN24 – Heparin oligosaccharide DP10

Heparine oligosaccharide of 10 sugar units (5 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 10 sugar units (5 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 10 sugar units (5 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 3 kDa Sus scrofa domesticus
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 May 6
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.3 nm
Dmax 5.7 nm

SASDN34 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, pH 7.4

Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.5 nm
Dmax 8.5 nm

SASDN44 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4

Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.5 nm
Dmax 6.8 nm

SASDN54 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4

Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.4 nm
Dmax 8.6 nm

SASDN64 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4

Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.5 nm
Dmax 6.8 nm

SASDN74 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.8 nm
Dmax 6.3 nm

SASDN84 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.8 nm
Dmax 7.7 nm

SASDN94 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.8 nm
Dmax 7.9 nm

SASDNA4 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.8 nm
Dmax 7.5 nm

SASDNB4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 1.25 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 4.8 nm
Dmax 29.7 nm

SASDNC4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 2.5 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 4.8 nm
Dmax 23.9 nm

SASDND4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 5mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.7 nm
Dmax 23.7 nm

SASDNE4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 10 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 2.4 nm
Dmax 20.8 nm

SASDNF4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 20 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.4 nm
Dmax 22.2 nm

SASDNG4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 40 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.0 nm
Dmax 22.1 nm

SASDNH4 – Heparin oligosaccharide in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 4.6 nm
Dmax 23.9 nm

SASDNJ4 – Heparin oligosaccharide in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 5.1 nm
Dmax 27.5 nm

SASDNK4 – Heparin oligosaccharide in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 5.1 nm
Dmax 30.8 nm

SASDNL4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl pH 7.4, 1.25 mg/mL

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.2 nm
Dmax 29.6 nm

SASDNM4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl pH 7.4, 2.5 mg/mL

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.2 nm
Dmax 26.2 nm

SASDNN4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl pH 7.4, 5 mg/mL

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.1 nm
Dmax 19.8 nm

SASDNP4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.9 nm
Dmax 22.2 nm

SASDNQ4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.8 nm
Dmax 22.7 nm

SASDNR4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.8 nm
Dmax 20.6 nm

SASDJ46 – Fep1-rec-GATA

GATA-type iron responsive transcription factor Fep1 reconstituted24-mer double strand DNA from the GATA promoter experimental SAS data
GATA-type iron responsive transcription factor Fep1 reconstituted 24-mer double strand DNA from the GATA promoter Kratky plot
Sample: GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein
24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.5 nm
Dmax 13.1 nm
VolumePorod 73 nm3

SASDJ56 – Fep1-GATA

GATA-type iron responsive transcription factor Fep124-mer double strand DNA from the GATA promoter experimental SAS data
GATA-type iron responsive transcription factor Fep1 24-mer double strand DNA from the GATA promoter Kratky plot
Sample: GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein
24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 85 nm3

SASDJ66 – GATA double strand DNA

24-mer double strand DNA from the GATA promoter experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 10 nm3

SASDJ76 – Fep1 wild type with [2Fe2S] cluster reconstituted

GATA-type iron responsive transcription factor Fep1 reconstituted experimental SAS data
Fep1 wild type with [2Fe2S] cluster reconstituted Rg histogram
Sample: GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.8 nm
Dmax 16.5 nm
VolumePorod 53 nm3

SASDJ86 – Fep1 wild type

GATA-type iron responsive transcription factor Fep1 experimental SAS data
Fep1 wild type Rg histogram
Sample: GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.5 nm
Dmax 11.8 nm
VolumePorod 41 nm3