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51 hits found for Alex Plumridge

SASDC74 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 0ms

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.5 nm
Dmax 8.0 nm

SASDC94 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 10ms

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.3 nm
Dmax 7.2 nm

SASDCA4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 30ms

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.2 nm
Dmax 7.0 nm

SASDCB4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 100ms

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.3 nm
Dmax 7.1 nm

SASDCC4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 300ms

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.2 nm
Dmax 6.8 nm

SASDCD4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 1000ms

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.2 nm
Dmax 6.8 nm

SASDCE4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 3000ms

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.2 nm
Dmax 6.7 nm

SASDCF4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 20mM KCl

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 20mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.6 nm
Dmax 8.5 nm

SASDCG4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 100mM KCl

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 100mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.5 nm
Dmax 8.0 nm

SASDCH4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 200mm KCl

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 200mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.5 nm
Dmax 8.0 nm

SASDCJ4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 400mM KCl

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 400mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.4 nm
Dmax 8.0 nm

SASDCK4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 0.25mM MgCl2

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 20mM KCl 0.25mM MgCl2 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.4 nm
Dmax 7.6 nm

SASDCL4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 0.50mM MgCl2

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 0.5mM MgCl2 20mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.3 nm
Dmax 7.2 nm

SASDCM4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 1mM MgCl2

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 1mM MgCl2 20mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.2 nm
Dmax 7.2 nm

SASDBD6 – Single stranded poly-deoxythymidine DNA (30mer, dT30)

Poly-deoxythymidine (30mer) experimental SAS data
Single stranded poly-deoxythymidine DNA (30mer, dT30) Rg histogram
Sample: Poly-deoxythymidine (30mer) monomer, 9 kDa DNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Apr 1
Visualizing single-stranded nucleic acids in solution. Nucleic Acids Res 45(9):e66 (2017)
Plumridge A, Meisburger SP, Pollack L
RgGuinier 3.0 nm
Dmax 10.7 nm

SASDBE6 – Single stranded poly-deoxyadenosine DNA (30mer, dA30)

Poly-deoxyadenosine (30mer) experimental SAS data
Single stranded poly-deoxyadenosine DNA (30mer, dA30) Rg histogram
Sample: Poly-deoxyadenosine (30mer) monomer, 9 kDa DNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
Visualizing single-stranded nucleic acids in solution. Nucleic Acids Res 45(9):e66 (2017)
Plumridge A, Meisburger SP, Pollack L
RgGuinier 2.7 nm
Dmax 9.5 nm

SASDJ67 – SAM2 Riboswitch 20 mM NaCl

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 3.5 nm

SASDJ77 – SAM2 Riboswitch 50 mM NaCl

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 50 mM NaCl, 20 μM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 3.3 nm

SASDJ87 – SAM2 Riboswitch 100 mM NaCl

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 100 mM NaCl, 20 μM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 3.0 nm

SASDJ97 – SAM2 Riboswitch 200 mM NaCl

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 200 mM NaCl, 20μM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 3.0 nm

SASDJA7 – SAM2 Riboswitch 0.5 mM MgCl2

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 0.5 mM MgCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 3.2 nm

SASDJB7 – SAM2 Riboswitch 1 mM MgCl2

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 1mM MgCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.5 nm

SASDJC7 – SAM2 Riboswitch 2 mM MgCl2

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.2 nm

SASDJD7 – SAM2 Riboswitch 3 mM MgCl2

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3 mM MgCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.1 nm

SASDJE7 – SAM2 Riboswitch 3.75 mM MgCl2

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3.75 mM MgCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.1 nm

SASDJF7 – SAM2 Riboswitch 0.25 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 0.25 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.7 nm

SASDJG7 – SAM2 Riboswitch 1 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 1 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDJH7 – SAM2 Riboswitch 2 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 2 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDJJ7 – SAM2 Riboswitch 3 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDJK7 – SAM2 Riboswitch 3.75 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3.75 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDFA9 – Poly-adenosine rA30 in 20 mM NaCl

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.7 nm
Dmax 11.0 nm
VolumePorod 17 nm3

SASDFB9 – Poly-adenosine rA30 in 100 mM NaCl

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 100 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.4 nm
Dmax 10.7 nm
VolumePorod 14 nm3

SASDFC9 – Poly-adenosine rA30 in 200 mM NaCl

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 200 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.5 nm
Dmax 10.5 nm
VolumePorod 14 nm3

SASDFD9 – Poly-adenosine rA30 in 400 mM NaCl

Poly-adenosine experimental SAS data
Poly-adenosine Kratky plot
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 400 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 13 nm3

SASDFE9 – Poly-adenosine rA30 in 600 mM NaCl

Poly-adenosine experimental SAS data
Poly-adenosine Kratky plot
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 600 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.2 nm
Dmax 10.2 nm
VolumePorod 13 nm3

SASDFF9 – Poly-adenosine rA30 in 1 mM MgCl2

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 1 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Jul 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.5 nm
Dmax 10.4 nm
VolumePorod 12 nm3

SASDFG9 – Poly-adenosine rA30 in 2 mM MgCl2

Poly-adenosine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 2 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 12 nm3

SASDFH9 – Poly-adenosine rA30 in 5 mM MgCl2

Poly-adenosine experimental SAS data
Poly-adenosine Kratky plot
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.3 nm
Dmax 10.0 nm
VolumePorod 15 nm3

SASDFJ9 – Poly-uridine rU30 in 20 mM NaCl

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 3.0 nm
Dmax 12.0 nm
VolumePorod 29 nm3

SASDFK9 – Poly-uridine rU30 in 100 mM NaCl

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 100 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.7 nm
Dmax 12.7 nm
VolumePorod 22 nm3

SASDFL9 – Poly-uridine rU30 in 200 mM NaCl

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 200 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.5 nm
Dmax 11.0 nm
VolumePorod 15 nm3

SASDFM9 – Poly-uridine rU30 in 400 mM NaCl

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 400 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.3 nm
Dmax 10.5 nm
VolumePorod 14 nm3

SASDFN9 – Poly-uridine rU30 in 600 mM NaCl

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 600 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.3 nm
Dmax 10.0 nm
VolumePorod 14 nm3

SASDFP9 – Poly-uridine rU30 in 1 mM MgCl2

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 1 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.7 nm
Dmax 11.0 nm
VolumePorod 17 nm3

SASDFQ9 – Poly-uridine rU30 in 2 mM MgCl2

Poly-uridine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 2 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.6 nm
Dmax 10.5 nm
VolumePorod 16 nm3

SASDFR9 – Poly-uridine rU30 in 5 mM MgCl2

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 15 nm3

SASDFS9 – Poly-uridine rU30 in 10 mM MgCl2

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 10 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 14 nm3

SASDB39 – Single stranded poly-deoxythymidine DNA (30mer, dT30) in 100 mM NaCl

Poly-deoxythymidine (30mer) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-deoxythymidine (30mer) monomer, 9 kDa DNA
Buffer: 1mM Na MOPS, 100mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Res 45(7):3932-3943 (2017)
Plumridge A, Meisburger SP, Andresen K, Pollack L
RgGuinier 2.8 nm

SASDB29 – Single stranded poly-deoxyadenosine DNA (30mer, dA30) in 200 mM NaCl

Poly-deoxyadenosine (30mer) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-deoxyadenosine (30mer) monomer, 9 kDa DNA
Buffer: 1mM Na MOPS, 200mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Res 45(7):3932-3943 (2017)
Plumridge A, Meisburger SP, Andresen K, Pollack L
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 15 nm3

SASDB49 – Single stranded poly-deoxythymidine DNA (30mer, dT30) in 200 mM NaCl

Poly-deoxythymidine (30mer) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-deoxythymidine (30mer) monomer, 9 kDa DNA
Buffer: 1mM Na MOPS, 200mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Res 45(7):3932-3943 (2017)
Plumridge A, Meisburger SP, Andresen K, Pollack L
RgGuinier 2.7 nm

SASDBZ8 – Single stranded poly-deoxyadenosine DNA (30mer, dA30) in 100 mM NaCl

Poly-deoxyadenosine (30mer) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Poly-deoxyadenosine (30mer) monomer, 9 kDa DNA
Buffer: 1mM Na MOPS, 100mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Res 45(7):3932-3943 (2017)
Plumridge A, Meisburger SP, Andresen K, Pollack L
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 16 nm3