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16 hits found for Aupič

SASDKQ2 – TET12SN(2CC)

TET12(1.10)SN-f5(2CC) experimental SAS data
DAMMIF model
Sample: TET12(1.10)SN-f5(2CC) monomer, 55 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 13
Designed folding pathway of modular coiled-coil-based proteins. Nat Commun 12(1):940 (2021)
Aupič J, Strmšek Ž, Lapenta F, Pahovnik D, Pisanski T, Drobnak I, Ljubetič A, Jerala R
RgGuinier 3.4 nm
Dmax 9.6 nm
VolumePorod 158 nm3

SASDKR2 – TET12SN(22CC)

TET12(1.10)SN-f5(22CC) experimental SAS data
DAMMIF model
Sample: TET12(1.10)SN-f5(22CC) monomer, 55 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 5
Designed folding pathway of modular coiled-coil-based proteins. Nat Commun 12(1):940 (2021)
Aupič J, Strmšek Ž, Lapenta F, Pahovnik D, Pisanski T, Drobnak I, Ljubetič A, Jerala R
RgGuinier 3.4 nm
Dmax 10.4 nm
VolumePorod 165 nm3

SASDKS2 – TET12SN(222CC)

TET12(1.10)SN-f5(222CC) experimental SAS data
MODELLER model
Sample: TET12(1.10)SN-f5(222CC) monomer, 55 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 15
Designed folding pathway of modular coiled-coil-based proteins. Nat Commun 12(1):940 (2021)
Aupič J, Strmšek Ž, Lapenta F, Pahovnik D, Pisanski T, Drobnak I, Ljubetič A, Jerala R
RgGuinier 3.4 nm
Dmax 10.6 nm
VolumePorod 170 nm3

SASDXJ3 – Human major vault protein

Major vault protein experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Major vault protein , 7747 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 8
Structural flexibility of the human vault particle revealed by high-resolution cryo-EM and molecular dynamics simulations. Nat Commun (2026)
...Aupič J, Marechal N, Durand A, Moussaoui D, Covaceuszach S, Gangupam B, D'Ercole C, Parra C, Cotugno D, Tomaino G, Tortora P, de Marco A, Cassetta A, Magistrato A, Hummer G
RgGuinier 16.3 nm
Dmax 65.0 nm
VolumePorod 16650 nm3

SASDJU5 – BIP18SN

BIP18SN experimental SAS data
MODELLER model
Sample: BIP18SN monomer, 80 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Nov 28
Self-assembly and regulation of protein cages from pre-organised coiled-coil modules. Nat Commun 12(1):939 (2021)
...Aupič J, Vezzoli M, Strmšek Ž, Da Vela S, Svergun DI, Carazo JM, Melero R, Jerala R
RgGuinier 4.6 nm
Dmax 12.4 nm

SASDJV5 – SBP12(9.b)

SBP1(9.b)SBP2(9.b) experimental SAS data
MODELLER model
Sample: SBP1(9.b) monomer, 40 kDa synthetic construct protein
SBP2(9.b) monomer, 40 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 19
Self-assembly and regulation of protein cages from pre-organised coiled-coil modules. Nat Commun 12(1):939 (2021)
...Aupič J, Vezzoli M, Strmšek Ž, Da Vela S, Svergun DI, Carazo JM, Melero R, Jerala R
RgGuinier 4.0 nm
Dmax 11.8 nm

SASDBS9 – TET12SN

TET12(1.10)SN-f5 experimental SAS data
CHIMERA model
Sample: TET12(1.10)SN-f5 monomer, 53 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Oct 28
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.5 nm
Dmax 12.0 nm
VolumePorod 107 nm3

SASDCT7 – PYR16(4.6)SN-f5

PYR16(4.6)SN-f5 experimental SAS data
MODELLER model
Sample: PYR16(4.6)SN-f5 monomer, 72 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 16
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.8 nm
Dmax 11.3 nm
VolumePorod 136 nm3

SASDCU7 – TRIP18(7.5R)SN-f5

TRIP18(7.5R)SN-f5 experimental SAS data
MODELLER model
Sample: TRIP18(7.5R)SN-f5 monomer, 82 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 4.2 nm
Dmax 14.5 nm
VolumePorod 210 nm3

SASDCR7 – TET12(1.10)SN-c6

TET12(1.10)SN-c6 experimental SAS data
MODELLER model
Sample: TET12(1.10)SN-c6 monomer, 54 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.6 nm
Dmax 11.5 nm
VolumePorod 145 nm3

SASDCS7 – TET12(1.10)SN-f9

TET12(1.10)SN-f9 experimental SAS data
MODELLER model
Sample: TET12(1.10)SN-f9 monomer, 57 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.9 nm
Dmax 12.9 nm
VolumePorod 153 nm3

SASDCV7 – TET12(2.3)SN-f5b

TET12(2.3)SN-f5b experimental SAS data
MODELLER model
Sample: TET12(2.3)SN-f5b monomer, 57 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.5 nm
Dmax 11.5 nm
VolumePorod 105 nm3

SASDCW7 – TET12(1.10)S-f5

TET12(1.10)S-f5 experimental SAS data
MODELLER model
Sample: TET12(1.10)S-f5 monomer, 53 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 7
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.6 nm
Dmax 13.5 nm
VolumePorod 210 nm3

SASDCX7 – TET12(1.10)S-f5b

TET12(1.10)S-f5b experimental SAS data
MODELLER model
Sample: TET12(1.10)S-f5b monomer, 53 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 7
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.4 nm
Dmax 11.2 nm
VolumePorod 127 nm3

SASDCY7 – TET12(1.10)S-c6

TET12(1.10)S-c6 experimental SAS data
MODELLER model
Sample: TET12(1.10)S-c6 monomer, 55 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 7
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.8 nm
Dmax 13.0 nm
VolumePorod 107 nm3

SASDCZ7 – TET12(1.6)S-c6b

TET12(1.6)S-c6b experimental SAS data
MODELLER model
Sample: TET12(1.6)S-c6b monomer, 54 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Apr 7
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.5 nm
Dmax 11.5 nm
VolumePorod 114 nm3