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15 hits found for Aupič

SASDKQ2 – TET12SN(2CC)

TET12(1.10)SN-f5(2CC) experimental SAS data
DAMMIF model
Sample: TET12(1.10)SN-f5(2CC) monomer, 55 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 13
Designed folding pathway of modular coiled-coil-based proteins. Nat Commun 12(1):940 (2021)
Aupič J, Strmšek Ž, Lapenta F, Pahovnik D, Pisanski T, Drobnak I, Ljubetič A, Jerala R
RgGuinier 3.4 nm
Dmax 9.6 nm
VolumePorod 158 nm3

SASDKR2 – TET12SN(22CC)

TET12(1.10)SN-f5(22CC) experimental SAS data
DAMMIF model
Sample: TET12(1.10)SN-f5(22CC) monomer, 55 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 5
Designed folding pathway of modular coiled-coil-based proteins. Nat Commun 12(1):940 (2021)
Aupič J, Strmšek Ž, Lapenta F, Pahovnik D, Pisanski T, Drobnak I, Ljubetič A, Jerala R
RgGuinier 3.4 nm
Dmax 10.4 nm
VolumePorod 165 nm3

SASDKS2 – TET12SN(222CC)

TET12(1.10)SN-f5(222CC) experimental SAS data
MODELLER model
Sample: TET12(1.10)SN-f5(222CC) monomer, 55 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 15
Designed folding pathway of modular coiled-coil-based proteins. Nat Commun 12(1):940 (2021)
Aupič J, Strmšek Ž, Lapenta F, Pahovnik D, Pisanski T, Drobnak I, Ljubetič A, Jerala R
RgGuinier 3.4 nm
Dmax 10.6 nm
VolumePorod 170 nm3

SASDJU5 – BIP18SN

BIP18SN experimental SAS data
MODELLER model
Sample: BIP18SN monomer, 80 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Nov 28
Self-assembly and regulation of protein cages from pre-organised coiled-coil modules. Nat Commun 12(1):939 (2021)
...Aupič J, Vezzoli M, Strmšek Ž, Da Vela S, Svergun DI, Carazo JM, Melero R, Jerala R
RgGuinier 4.6 nm
Dmax 12.4 nm

SASDJV5 – SBP12(9.b)

SBP1(9.b)SBP2(9.b) experimental SAS data
MODELLER model
Sample: SBP1(9.b) monomer, 40 kDa synthetic construct protein
SBP2(9.b) monomer, 40 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 19
Self-assembly and regulation of protein cages from pre-organised coiled-coil modules. Nat Commun 12(1):939 (2021)
...Aupič J, Vezzoli M, Strmšek Ž, Da Vela S, Svergun DI, Carazo JM, Melero R, Jerala R
RgGuinier 4.0 nm
Dmax 11.8 nm

SASDBS9 – TET12SN

TET12(1.10)SN-f5 experimental SAS data
CHIMERA model
Sample: TET12(1.10)SN-f5 monomer, 53 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Oct 28
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.5 nm
Dmax 12.0 nm
VolumePorod 107 nm3

SASDCT7 – PYR16(4.6)SN-f5

PYR16(4.6)SN-f5 experimental SAS data
MODELLER model
Sample: PYR16(4.6)SN-f5 monomer, 72 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 16
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.8 nm
Dmax 11.3 nm
VolumePorod 136 nm3

SASDCU7 – TRIP18(7.5R)SN-f5

TRIP18(7.5R)SN-f5 experimental SAS data
MODELLER model
Sample: TRIP18(7.5R)SN-f5 monomer, 82 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 4.2 nm
Dmax 14.5 nm
VolumePorod 210 nm3

SASDCR7 – TET12(1.10)SN-c6

TET12(1.10)SN-c6 experimental SAS data
MODELLER model
Sample: TET12(1.10)SN-c6 monomer, 54 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.6 nm
Dmax 11.5 nm
VolumePorod 145 nm3

SASDCS7 – TET12(1.10)SN-f9

TET12(1.10)SN-f9 experimental SAS data
MODELLER model
Sample: TET12(1.10)SN-f9 monomer, 57 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.9 nm
Dmax 12.9 nm
VolumePorod 153 nm3

SASDCV7 – TET12(2.3)SN-f5b

TET12(2.3)SN-f5b experimental SAS data
MODELLER model
Sample: TET12(2.3)SN-f5b monomer, 57 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.5 nm
Dmax 11.5 nm
VolumePorod 105 nm3

SASDCW7 – TET12(1.10)S-f5

TET12(1.10)S-f5 experimental SAS data
MODELLER model
Sample: TET12(1.10)S-f5 monomer, 53 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 7
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.6 nm
Dmax 13.5 nm
VolumePorod 210 nm3

SASDCX7 – TET12(1.10)S-f5b

TET12(1.10)S-f5b experimental SAS data
MODELLER model
Sample: TET12(1.10)S-f5b monomer, 53 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 7
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.4 nm
Dmax 11.2 nm
VolumePorod 127 nm3

SASDCY7 – TET12(1.10)S-c6

TET12(1.10)S-c6 experimental SAS data
MODELLER model
Sample: TET12(1.10)S-c6 monomer, 55 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 7
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.8 nm
Dmax 13.0 nm
VolumePorod 107 nm3

SASDCZ7 – TET12(1.6)S-c6b

TET12(1.6)S-c6b experimental SAS data
MODELLER model
Sample: TET12(1.6)S-c6b monomer, 54 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Apr 7
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
...Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
RgGuinier 3.5 nm
Dmax 11.5 nm
VolumePorod 114 nm3