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9 hits found for Hutin

SASDG44 – Adenovirus Ad3 fibre knob in complex with the EC2-EC3 fragment of desmoglein 2

Human adenovirus serotype 3 fibre knobHuman desmoglein 2 extracellular domain 2 and 3 experimental SAS data
OTHER model
Sample: Human adenovirus serotype 3 fibre knob trimer, 69 kDa Human adenovirus serotype … protein
Human desmoglein 2 extracellular domain 2 and 3 dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 3 mM CaCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Jan 26
Intermediate-resolution crystal structure of the human adenovirus B serotype 3 fibre knob in complex with the EC2-EC3 fragment of desmoglein 2. Acta Crystallogr F Struct Biol Commun 75(Pt 12):750-757 (2019)
...Hutin S, Fender P, Burmeister WP
RgGuinier 3.4 nm
Dmax 11.2 nm
VolumePorod 188 nm3

SASDBU4 – Vaccinia primase D5 protein fragment containing the D5N and helicase domain

Primase D5 protein fragment containing the D5N and helicase domain experimental SAS data
DAMMIF model
Sample: Primase D5 protein fragment containing the D5N and helicase domain hexamer, 321 kDa Vaccinia virus protein
Buffer: 20 mM Tris 150 mM NaCL 5% glycerol 1mM DTT, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 26
Domain Organization of Vaccinia Virus Helicase-Primase D5. J Virol 90(9):4604-4613 (2016)
Hutin S, Ling WL, Round A, Effantin G, Reich S, Iseni F, Tarbouriech N, Schoehn G, Burmeister WP
RgGuinier 4.8 nm
Dmax 14.5 nm
VolumePorod 570 nm3

SASDCM5 – Vaccinia virus binary E9 polymerase / DNA complex

DNA polymerase E9 exo- mutantDNA oligomer template-primer hairpin experimental SAS data
PYMOL model
Sample: DNA polymerase E9 exo- mutant monomer, 117 kDa Vaccinia virus protein
DNA oligomer template-primer hairpin monomer, 9 kDa synthetic construct DNA
Buffer: 20 mM Tris HCl, 100 mM NaCl, 4 mM DTT, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 12
The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding. Nat Commun 8(1):1455 (2017)
...Hutin S, Mas PJ, Man P, Forest E, Hart DJ, Peyrefitte CN, Burmeister WP, Iseni F
RgGuinier 3.5 nm
Dmax 12.0 nm
VolumePorod 210 nm3

SASDCN5 – Vaccinia virus DNA polymerase

DNA polymerase E9 experimental SAS data
PYMOL model
Sample: DNA polymerase E9 monomer, 117 kDa Vaccinia virus protein
Buffer: 20 mM Tris HCl, 100 mM NaCl, 4 mM DTT, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2014 Nov 27
The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding. Nat Commun 8(1):1455 (2017)
...Hutin S, Mas PJ, Man P, Forest E, Hart DJ, Peyrefitte CN, Burmeister WP, Iseni F
RgGuinier 3.9 nm
Dmax 12.0 nm
VolumePorod 202 nm3

SASDCP5 – Vaccinia virus DNA polymerase processivity factor component A20 (C-terminal fragment)

DNA polymerase processivity factor component A20 C-ter fragment experimental SAS data
DAMMIF model
Sample: DNA polymerase processivity factor component A20 C-ter fragment monomer, 17 kDa Vaccinia virus protein
Buffer: 25 mM Tris-HCl, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 5
The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding. Nat Commun 8(1):1455 (2017)
...Hutin S, Mas PJ, Man P, Forest E, Hart DJ, Peyrefitte CN, Burmeister WP, Iseni F
RgGuinier 2.2 nm
Dmax 7.4 nm
VolumePorod 31 nm3

SASDY37 – MADS transcription factor SEPALLATA 3 (SEP3)

Developmental protein SEPALLATA 3 experimental SAS data
Developmental protein SEPALLATA 3 Kratky plot
Sample: Developmental protein SEPALLATA 3 dimer, 24 kDa Arabidopsis thaliana protein
Buffer: 50 mM Tris, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Feb 23
The phytoplasma effector, phyllogen, structurally mimics host plant MADS transcription factors. J Biol Chem 302(10):113449 (2026)
...Hutin S, Le Hir S, Paul M, Albanese P, Kieffer-Jaquinod S, Guillotin A, Iwabuchi N, Maejima K, Nanao MH, Tully MD, Hugouvieux V, Zubieta C
RgGuinier 4.0 nm
Dmax 15.4 nm
VolumePorod 530 nm3

SASDY47 – Phytoplasmal effector causing phyllody 1 (Phyl1)

Phytoplasmal effector causing phyllody 1 experimental SAS data
Phytoplasmal effector causing phyllody 1 Kratky plot
Sample: Phytoplasmal effector causing phyllody 1 monomer, 11 kDa Onion yellows phytoplasma … protein
Buffer: 50 mM Tris, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Feb 23
The phytoplasma effector, phyllogen, structurally mimics host plant MADS transcription factors. J Biol Chem 302(10):113449 (2026)
...Hutin S, Le Hir S, Paul M, Albanese P, Kieffer-Jaquinod S, Guillotin A, Iwabuchi N, Maejima K, Nanao MH, Tully MD, Hugouvieux V, Zubieta C
RgGuinier 2.2 nm
Dmax 6.6 nm
VolumePorod 51 nm3

SASDY57 – MADS transcription factor SEPALLATA 3 (SEP3) bound to Phytoplasmal effector causing phyllody 1

Developmental protein SEPALLATA 3Phytoplasmal effector causing phyllody 1 experimental SAS data
Developmental protein SEPALLATA 3 Phytoplasmal effector causing phyllody 1 Kratky plot
Sample: Developmental protein SEPALLATA 3 dimer, 24 kDa Arabidopsis thaliana protein
Phytoplasmal effector causing phyllody 1 monomer, 11 kDa Onion yellows phytoplasma … protein
Buffer: 50 mM Tris, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Feb 23
The phytoplasma effector, phyllogen, structurally mimics host plant MADS transcription factors. J Biol Chem 302(10):113449 (2026)
...Hutin S, Le Hir S, Paul M, Albanese P, Kieffer-Jaquinod S, Guillotin A, Iwabuchi N, Maejima K, Nanao MH, Tully MD, Hugouvieux V, Zubieta C
RgGuinier 3.8 nm
Dmax 13.6 nm
VolumePorod 200 nm3

SASDSW8 – Apo form of vaccinia virus polymerase holoenzyme E9-A20-D4

DNA polymeraseDNA polymerase processivity factor component OPG148Uracil-DNA glycosylase experimental SAS data
DNA polymerase DNA polymerase processivity factor component OPG148 Uracil-DNA glycosylase Kratky plot
Sample: DNA polymerase monomer, 117 kDa Vaccinia virus (strain … protein
DNA polymerase processivity factor component OPG148 monomer, 49 kDa Vaccinia virus (strain … protein
Uracil-DNA glycosylase monomer, 27 kDa Vaccinia virus (strain … protein
Buffer: 200 mM NaCl, 20 mM Tris-HCl and 1 mM TCEP, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 25
Structure and flexibility of the DNA polymerase holoenzyme of vaccinia virus. PLoS Pathog 20(5):e1011652 (2024)
...Hutin S, Kraft C, Grimm C, Böttcher B, Fischer U, Tarbouriech N, Iseni F
RgGuinier 5.0 nm
Dmax 18.0 nm
VolumePorod 350 nm3