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107 hits found for Pabit

SASDKT2 – Wild type double Element for Nuclear Expression (dENE)

Wild type dENE experimental SAS data
Wild type dENE Kratky plot
Sample: Wild type dENE monomer, 23 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.8 nm
Dmax 10.8 nm
VolumePorod 31 nm3

SASDKU2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)20

Wild type dENE + Poly(A)20 experimental SAS data
Wild type dENE + Poly(A)20 Kratky plot
Sample: Wild type dENE + Poly(A)20 monomer, 30 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.8 nm
Dmax 10.7 nm

SASDKV2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)25

Wild type dENE + Poly(A)25 experimental SAS data
Wild type dENE + Poly(A)25 Kratky plot
Sample: Wild type dENE + Poly(A)25 monomer, 31 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.4 nm

SASDKW2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)30

Wild type dENE + Poly(A)30 experimental SAS data
Wild type dENE + Poly(A)30 Kratky plot
Sample: Wild type dENE + Poly(A)30 monomer, 33 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.6 nm

SASDKX2 – double Element for Nuclear Expression (dENE) Mutant A

A dENE experimental SAS data
A dENE Kratky plot
Sample: A dENE monomer, 27 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 10.9 nm

SASDKY2 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)20

A dENE + Poly(A)20 experimental SAS data
A dENE + Poly(A)20 Kratky plot
Sample: A dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.5 nm

SASDK23 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)30

A dENE + Poly(A)30 experimental SAS data
A dENE + Poly(A)30 Kratky plot
Sample: A dENE + Poly(A)30 monomer, 37 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 13.3 nm

SASDK33 – double Element for Nuclear Expression (dENE) Mutant B

B dENE experimental SAS data
B dENE Kratky plot
Sample: B dENE monomer, 24 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.8 nm

SASDK43 – double Element for Nuclear Expression (dENE) Mutant B + Poly(A)30

B dENE + Poly(A)30 experimental SAS data
B dENE + Poly(A)30 Kratky plot
Sample: B dENE + Poly(A)30 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.8 nm

SASDK63 – double Element for Nuclear Expression (dENE) Mutant C

C dENE experimental SAS data
C dENE Kratky plot
Sample: C dENE monomer, 29 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.4 nm
Dmax 12.4 nm

SASDK73 – double Element for Nuclear Expression (dENE) Crystal Structure

Xtal dENE experimental SAS data
Xtal dENE Kratky plot
Sample: Xtal dENE monomer, 28 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.1 nm
Dmax 11.0 nm

SASDK83 – double Element for Nuclear Expression (dENE) Mutant C + Poly(A)30

C dENE + Poly(A)30 experimental SAS data
C dENE + Poly(A)30 Kratky plot
Sample: C dENE + Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.6 nm
Dmax 14.8 nm

SASDKA3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)25

Xtal dENE + Poly(A)25 experimental SAS data
Xtal dENE + Poly(A)25 Kratky plot
Sample: Xtal dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKB3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)30

Xtal dENE + Poly(A)30 experimental SAS data
Xtal dENE + Poly(A)30 Kratky plot
Sample: Xtal dENE + Poly(A)30 monomer, 38 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 13.2 nm

SASDKC3 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)25

A dENE + Poly(A)25 experimental SAS data
A dENE + Poly(A)25 Kratky plot
Sample: A dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKD3 – double Element for Nuclear Expression (dENE) Mutant B + Hairpin - Poly(A)30

B dENE + Hairpin - Poly(A)30 experimental SAS data
B dENE + Hairpin - Poly(A)30 Kratky plot
Sample: B dENE + Hairpin - Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 14.0 nm

SASDKE3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)20

Xtal dENE + Poly(A)20 experimental SAS data
Xtal dENE + Poly(A)20 Kratky plot
Sample: Xtal dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.8 nm

SASDRE3 – Tissue Transglutaminase + Ca: Time-resolved 0ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.1 nm
Dmax 16.0 nm
VolumePorod 150 nm3

SASDRF3 – Tissue Transglutaminase + Ca: Time-resolved 32 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.1 nm
Dmax 17.0 nm
VolumePorod 170 nm3

SASDRG3 – Tissue Transglutaminase + Ca: Time-resolved 63 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.1 nm
Dmax 18.0 nm
VolumePorod 155 nm3

SASDRH3 – Tissue Transglutaminase + Ca: Time-resolved 100 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.1 nm
Dmax 20.0 nm
VolumePorod 190 nm3

SASDRJ3 – Tissue Transglutaminase + Ca: Time-resolved 316 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.5 nm
Dmax 24.0 nm
VolumePorod 200 nm3

SASDRK3 – Tissue Transglutaminase + Ca: Time-resolved 631 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.6 nm
Dmax 26.0 nm
VolumePorod 215 nm3

SASDRL3 – Tissue Transglutaminase + Ca: Time-resolved 1500 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 4.6 nm
Dmax 28.0 nm
VolumePorod 230 nm3

SASDTL3 – Tissue Transglutaminase: Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.2 nm
Dmax 20.0 nm
VolumePorod 148 nm3

SASDRM3 – Tissue Transglutaminase + Ca: 5min Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Sep 29
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 5.0 nm
Dmax 27.0 nm
VolumePorod 241 nm3

SASDTM3 – Tissue Transglutaminase + 25 µM GTP

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.8 nm
Dmax 17.5 nm
VolumePorod 132 nm3

SASDRN3 – Tissue Transglutaminase + Ca: 10min Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 5.5 nm
Dmax 26.0 nm
VolumePorod 300 nm3

SASDTN3 – Tissue Transglutaminase + 50 µM GTP

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.7 nm
Dmax 17.5 nm
VolumePorod 124 nm3

SASDRP3 – Tissue Transglutaminase + Ca: 30min Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Sep 29
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 6.9 nm
Dmax 31.5 nm
VolumePorod 490 nm3

SASDTP3 – Tissue Transglutaminase + 100 µM GTP

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.6 nm
Dmax 18.0 nm
VolumePorod 121 nm3

SASDRQ3 – Trypsin + Aprotinin: Time-Resolved 10 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 23 nm3

SASDTQ3 – Tissue Transglutaminase + 250 µM GTP

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.5 nm
Dmax 17.5 nm
VolumePorod 120 nm3

SASDRR3 – Trypsin + Aprotinin: Time-Resolved 30 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 28 nm3

SASDTR3 – Tissue Transglutaminase + 500 µM GTP

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.4 nm
Dmax 18.0 nm
VolumePorod 118 nm3

SASDRS3 – Trypsin + Aprotinin: Time-Resolved 100 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.8 nm
Dmax 5.3 nm
VolumePorod 30 nm3

SASDTS3 – Tissue Transglutaminase + 1 mM GTP

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.4 nm
Dmax 17.0 nm
VolumePorod 115 nm3

SASDRT3 – Trypsin + Aprotinin: Time-Resolved 400 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 27 nm3

SASDTT3 – Tissue Transglutaminase + 2 mM GTP

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.3 nm
Dmax 17.0 nm
VolumePorod 111 nm3

SASDRU3 – Trypsin + Aprotinin: Time-Resolved 630 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 25 nm3

SASDTU3 – Tissue Transglutaminase + 5 mM GTP: Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 5 mM GTP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.2 nm
Dmax 14.0 nm
VolumePorod 108 nm3

SASDRV3 – Trypsin + Aprotinin: Time-Resolved 1000 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.8 nm
Dmax 5.3 nm
VolumePorod 28 nm3

SASDTV3 – Tissue Transglutaminase + 5 mM GDP

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.5 nm
Dmax 17.5 nm
VolumePorod 120 nm3

SASDRW3 – Trypsin + Aprotinin: Time-Resolved 2000 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.0 nm
Dmax 5.3 nm
VolumePorod 29 nm3

SASDTW3 – Tissue Transglutaminase + 250 µM CaCl2

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.3 nm
Dmax 21.0 nm
VolumePorod 160 nm3

SASDRX3 – Trypsin + Aprotinin: Complex equilibrium

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 31 nm3

SASDTX3 – Tissue Transglutaminase + 500 µM CaCl2

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.3 nm
Dmax 20.0 nm
VolumePorod 158 nm3

SASDRY3 – Kenics GAC rRNA + Mg: Time-Resolved 10 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 10.0 nm
VolumePorod 28 nm3

SASDTY3 – Tissue Transglutaminase + 1 mM CaCl2

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.5 nm
Dmax 24.0 nm
VolumePorod 180 nm3

SASDRZ3 – Kenics GAC rRNA + Mg: Time-Resolved 32 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 10.0 nm
VolumePorod 27 nm3

SASDTZ3 – Tissue Transglutaminase + 2 mM CaCl2

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.3 nm
Dmax 25.5 nm
VolumePorod 234 nm3

SASDR24 – Kenics GAC rRNA + Mg: Time-Resolved 63 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 9.5 nm
VolumePorod 27 nm3

SASDT24 – Tissue Transglutaminase + 50 µM inhibitor LM11

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.3 nm
Dmax 20.0 nm
VolumePorod 158 nm3

SASDR34 – Kenics GAC rRNA + Mg: Time-Resolved 100 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 10.0 nm
VolumePorod 28 nm3

SASDT34 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 250 µM CaCl2

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.2 nm
Dmax 19.0 nm
VolumePorod 155 nm3

SASDR44 – Kenics GAC rRNA + Mg: Time-Resolved 316 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 27 nm3

SASDT44 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 500 µM CaCl2

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.9 nm
Dmax 25.0 nm
VolumePorod 191 nm3

SASDR54 – Kenics GAC rRNA + Mg: Time-Resolved 631 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.3 nm
Dmax 9.8 nm
VolumePorod 26 nm3

SASDT54 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 1 mM CaCl2

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.7 nm
Dmax 28.0 nm
VolumePorod 198 nm3

SASDR64 – Kenics GAC rRNA + Mg: Time-Resolved 1000 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.3 nm
Dmax 11.0 nm
VolumePorod 26 nm3

SASDT64 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 2 mM CaCl2

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.2 nm
Dmax 25.0 nm
VolumePorod 236 nm3

SASDR74 – Kenics GAC rRNA + Mg: Equilibrium initial

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 32 nm3

SASDT74 – Tissue Transglutaminase + CaCl2 + inhibitor LM11: Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM LM11, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 6.0 nm
Dmax 36.0 nm
VolumePorod 431 nm3

SASDR84 – Kenics GAC rRNA + Mg: Equilibrium final

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 10.0 nm
VolumePorod 26 nm3

SASDT84 – Tissue Transglutaminase + inhibitor LM11 + CaCl2: Time-resolved 100 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.6 nm
Dmax 32.0 nm
VolumePorod 242 nm3

SASDR94 – GAC rRNA + L11 Protein: Time-Resolved 30 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 12.0 nm
VolumePorod 34 nm3

SASDT94 – Tissue Transglutaminase + inhibitor LM11 + CaCl2: Time-resolved 316 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.8 nm
Dmax 25.0 nm
VolumePorod 203 nm3

SASDRA4 – GAC rRNA + L11 Protein: Time-Resolved 50 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 13.5 nm
VolumePorod 36 nm3

SASDTA4 – Tissue Transglutaminase + inhibitor LM11 + CaCl2: Time-resolved 631 ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.7 nm
Dmax 33.0 nm
VolumePorod 305 nm3

SASDRB4 – GAC rRNA + L11 Protein: Time-Resolved 63 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 11.5 nm
VolumePorod 33 nm3

SASDTB4 – Tissue Transglutaminase + CaCl2 + inhibitor LM11 + GTP: Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM LM11, 5 mM GTP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 6.2 nm
Dmax 33.0 nm
VolumePorod 277 nm3

SASDRC4 – GAC rRNA + L11 Protein: Time-Resolved 100 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 11.5 nm
VolumePorod 35 nm3

SASDTC4 – Tissue Transglutaminase + 50 µM inhibitor 5826 (1% DMSO)

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.1 nm
Dmax 19.0 nm
VolumePorod 144 nm3

SASDRD4 – GAC rRNA + L11 Protein: Time-Resolved 200 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 12.5 nm
VolumePorod 37 nm3

SASDTD4 – Tissue Transglutaminase + 50 µM inhibitor 5826 (2% DMSO)

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.9 nm
Dmax 18.5 nm
VolumePorod 134 nm3

SASDRE4 – GAC rRNA + L11 Protein: Time-Resolved 316 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 38 nm3

SASDTE4 – Tissue Transglutaminase + 100 µM inhibitor 5826 (2% DMSO)

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.8 nm
Dmax 17.6 nm
VolumePorod 130 nm3

SASDRF4 – GAC rRNA + L11 Protein: Time-Resolved 631 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 13.0 nm
VolumePorod 38 nm3

SASDTF4 – Tissue Transglutaminase + CaCl2 + inhibitor 5826: Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM 5826 inhibitor, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 6.7 nm
Dmax 39.0 nm
VolumePorod 450 nm3

SASDRG4 – GAC rRNA + L11 Protein: Time-Resolved 1000 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 12.5 nm
VolumePorod 40 nm3

SASDTG4 – Tissue Transglutaminase + CaCl2 + inhibitor 5826 + GTP: Equilibrium

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM 5826, 5 mM GTP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 6.0 nm
Dmax 29.0 nm
VolumePorod 248 nm3

SASDRH4 – GAC rRNA + L11 Protein: Time-Resolved 2000 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.6 nm
Dmax 12.8 nm
VolumePorod 39 nm3

SASDTH4 – Tissue Transglutaminase + 1% DMSO

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.0 nm
Dmax 18.5 nm
VolumePorod 139 nm3

SASDRJ4 – GAC rRNA + L11 Protein: SEC-SAXS final

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.6 nm
Dmax 13.0 nm
VolumePorod 48 nm3

SASDTJ4 – Tissue Transglutaminase + 2% DMSO

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.9 nm
Dmax 18.5 nm
VolumePorod 133 nm3

SASDRK4 – Diffusive GAC rRNA + Mg: Time-Resolved 10 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 29 nm3

SASDTK4 – Tissue Transglutaminase + 5% DMSO

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.9 nm
Dmax 18.5 nm
VolumePorod 133 nm3

SASDRL4 – Diffusive GAC rRNA + Mg: Time-Resolved 30 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 29 nm3

SASDTL4 – Tissue Transglutaminase + 10% DMSO

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.1 nm
Dmax 21.5 nm
VolumePorod 148 nm3

SASDRM4 – Diffusive GAC rRNA + Mg: Time-Resolved 100 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 9.0 nm
VolumePorod 29 nm3

SASDTM4 – Tissue Transglutaminase R580K mutant

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 6.1 nm
Dmax 32.0 nm
VolumePorod 488 nm3

SASDRN4 – Diffusive GAC rRNA + Mg: Time-Resolved 300 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 29 nm3

SASDTN4 – Tissue Transglutaminase R580K mutant + 100 µM GTP

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.9 nm
Dmax 27.0 nm
VolumePorod 381 nm3

SASDRP4 – Diffusive GAC rRNA + Mg: Time-Resolved 1000 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 13.0 nm
VolumePorod 30 nm3

SASDTP4 – Tissue Transglutaminase R580K mutant + 1 mM GTP

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.7 nm
Dmax 30.0 nm
VolumePorod 283 nm3

SASDRQ4 – Diffusive GAC rRNA + Mg: Equilibrium initial

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 34 nm3

SASDTQ4 – Tissue Transglutaminase R580K mutant + 2 mM GTP

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.3 nm
Dmax 25.0 nm
VolumePorod 264 nm3

SASDRR4 – Diffusive GAC rRNA + Mg: Equilibrium final

50S ribosomal protein L11 experimental SAS data
50S ribosomal protein L11 Kratky plot
Sample: 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 11.0 nm
VolumePorod 27 nm3

SASDTR4 – Tissue Transglutaminase R580K mutant + 5 mM GTP

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.2 nm
Dmax 25.0 nm
VolumePorod 243 nm3

SASDPU5 – Murine hepatitis virus (MHV) coronavirus putative packaging signal RNA

MHV coronavirus putative packaging signal RNA experimental SAS data
MHV coronavirus putative packaging signal RNA Kratky plot
Sample: MHV coronavirus putative packaging signal RNA monomer, 31 kDa Murine coronavirus RNA
Buffer: 70 mM KCl, 50 μM EDTA, 25 mM HEPES, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2021 Jun 10
MHV Coronavirus Packaging Signal RNA and N protein
Suzette Pabit
RgGuinier 3.9 nm
Dmax 12.0 nm
VolumePorod 58 nm3