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15 hits found for Sethi

SASDV64 – Serine hydrolase FphH with short chain esterase activity (Apo FphH)

Alpha/beta fold hydrolase experimental SAS data
DAMFILT model
Sample: Alpha/beta fold hydrolase monomer, 28 kDa Staphylococcus aureus protein
Buffer: 100 mM NaCl, 10 mM HEPES, pH: 7.6
Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2024 Apr 11
Similar but Distinct—Biochemical Characterization of the Staphylococcus aureus Serine Hydrolases FphH  and FphI Proteins: Structure, Function, and Bioinformatics (2024)
...Sethi A, Jelinek R, Kass I
RgGuinier 2.1 nm
Dmax 6.5 nm
VolumePorod 41 nm3

SASDXD4 – Full-length human SFPQ dimer (protiated and deuterated)

Splicing factor, proline- and glutamine-rich experimental SAS data
Splicing factor, proline- and glutamine-rich Kratky plot
Sample: Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer: 150 mM KCl, 1.5% glycerol, 20 mM HEPES, 1 mM DTT (in 100% H2O), pH: 7.4
Experiment: SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2023 Jan 23
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
...Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 8.7 nm
Dmax 30.7 nm
VolumePorod 241000 nm3

SASDQG4 – Rabies virus Nishigahara strain Phosphoprotein Isoform 3 (P3)

Isoform P3 of Phosphoprotein experimental SAS data
Rabies virus Nishigahara strain Phosphoprotein Isoform 3 (P3) Rg histogram
Sample: Isoform P3 of Phosphoprotein dimer, 55 kDa Rabies virus (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 26
Structural insights into the multifunctionality of rabies virus P3 protein. Proc Natl Acad Sci U S A 120(14):e2217066120 (2023)
Sethi A, Rawlinson SM, Dubey A, Ang CS, Choi YH, Yan F, Okada K, Rozario AM, Brice AM, Ito N, Williamson NA, Hatters DM, Bell TDM, Arthanari H, Moseley GW, Gooley PR
RgGuinier 3.7 nm
Dmax 16.5 nm
VolumePorod 108 nm3

SASDQH4 – Attenuated Nishigahara Phosphoprotein Isoform 3 (Ni-CE P3)

Attenuated derivative P3 of Phosphoprotein experimental SAS data
Attenuated Nishigahara Phosphoprotein Isoform 3 (Ni-CE P3) Rg histogram
Sample: Attenuated derivative P3 of Phosphoprotein dimer, 55 kDa protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 26
Structural insights into the multifunctionality of rabies virus P3 protein. Proc Natl Acad Sci U S A 120(14):e2217066120 (2023)
Sethi A, Rawlinson SM, Dubey A, Ang CS, Choi YH, Yan F, Okada K, Rozario AM, Brice AM, Ito N, Williamson NA, Hatters DM, Bell TDM, Arthanari H, Moseley GW, Gooley PR
RgGuinier 4.0 nm
Dmax 17.5 nm
VolumePorod 127 nm3

SASDQJ4 – N226H Nishigahara Phosphoprotein Isoform 3 (N226H_P3)

Isoform P3 of Phosphoprotein Nish P3 N226H experimental SAS data
N226H Nishigahara Phosphoprotein Isoform 3 (N226H_P3) Rg histogram
Sample: Isoform P3 of Phosphoprotein Nish P3 N226H dimer, 55 kDa protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 26
Structural insights into the multifunctionality of rabies virus P3 protein. Proc Natl Acad Sci U S A 120(14):e2217066120 (2023)
Sethi A, Rawlinson SM, Dubey A, Ang CS, Choi YH, Yan F, Okada K, Rozario AM, Brice AM, Ito N, Williamson NA, Hatters DM, Bell TDM, Arthanari H, Moseley GW, Gooley PR
RgGuinier 4.3 nm
Dmax 19.0 nm
VolumePorod 150 nm3

SASDV57 – Full-length SFPQ in high salt buffer

Splicing factor, proline- and glutamine-rich experimental SAS data
Splicing factor, proline- and glutamine-rich Kratky plot
Sample: Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer: 500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Oct 22
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
...Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 7.9 nm
Dmax 42.5 nm
VolumePorod 485 nm3

SASDV67 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707)

Splicing Factor Proline/Glutamine rich (1-598) experimental SAS data
Splicing Factor Proline/Glutamine rich (1-598) Kratky plot
Sample: Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein
Buffer: 500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 13
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
...Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 7.6 nm
Dmax 34.3 nm
VolumePorod 400 nm3

SASDV77 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707) in low salt buffer

Splicing Factor Proline/Glutamine rich (1-598) experimental SAS data
Splicing Factor Proline/Glutamine rich (1-598) Kratky plot
Sample: Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein
Buffer: 150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 13
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
...Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 6.1 nm
Dmax 28.0 nm
VolumePorod 315 nm3

SASDLG7 – LDLa linker region of human Relaxin receptor 1 (RXFP1, 23-94)

Relaxin receptor 1 experimental SAS data
LDLa linker region of human Relaxin receptor 1 (RXFP1, 23-94) Rg histogram
Sample: Relaxin receptor 1 monomer, 8 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.1%NaN3, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 6
Structural Insights into the Unique Modes of Relaxin-Binding and Tethered-Agonist Mediated Activation of RXFP1 and RXFP2. J Mol Biol 433(21):167217 (2021)
Sethi A, Bruell S, Ryan T, Yan F, Tanipour MH, Mok YF, Draper-Joyce C, Khandokar Y, Metcalfe RD, Griffin MDW, Scott DJ, Hossain MA, Petrie EJ, Bathgate RAD, Gooley PR
RgGuinier 2.1 nm
Dmax 10.0 nm
VolumePorod 19 nm3

SASDLH7 – LDLa linker region of human Relaxin receptor 2 (RXFP2, 38-105)

Relaxin receptor 2 experimental SAS data
LDLa linker region of human Relaxin receptor 2 (RXFP2, 38-105) Rg histogram
Sample: Relaxin receptor 2 monomer, 7 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.1%NaN3, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 6
Structural Insights into the Unique Modes of Relaxin-Binding and Tethered-Agonist Mediated Activation of RXFP1 and RXFP2. J Mol Biol 433(21):167217 (2021)
Sethi A, Bruell S, Ryan T, Yan F, Tanipour MH, Mok YF, Draper-Joyce C, Khandokar Y, Metcalfe RD, Griffin MDW, Scott DJ, Hossain MA, Petrie EJ, Bathgate RAD, Gooley PR
RgGuinier 1.7 nm
Dmax 7.1 nm
VolumePorod 12 nm3

SASDV59 – Human Splicing factor, proline- and glutamine-rich protein 5% SFPQ and 95% dSFPQ at a D2O matchpoint of 95%

Splicing factor, proline- and glutamine-rich experimental SAS data
Splicing factor, proline- and glutamine-rich Kratky plot
Sample: Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer: 150 mM KCl, 0.075% glycerol, 20 mM HEPES, 0.5 mM DTT,, pH: 7.4
Experiment: SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2023 Jan 20
Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
...Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C
RgGuinier 6.1 nm
Dmax 22.0 nm
VolumePorod 181 nm3

SASDRB6 – Rabies CVS Phosphoprotein (P1)

Phosphoprotein experimental SAS data
Rabies CVS Phosphoprotein (P1) Rg histogram
Sample: Phosphoprotein dimer, 67 kDa Rabies virus (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Mar 7
Conformational dynamics, RNA binding, and phase separation regulate the multifunctionality of rabies virus P protein. Nat Commun 16(1):9491 (2025)
...Sethi A, David CT, Harrison AR, Bird LE, Rozario AM, Uthishtran S, Ardipradja K, Zhao T, Oksayan S, Jans DA, Ang CS, Lu ZH, Yan F, Williamson NA, Arumugam S, Sundaramoorthy V, Bell TDM, Gooley PR, Mos...
RgGuinier 4.7 nm
Dmax 25.0 nm
VolumePorod 230 nm3

SASDRX4 – Rabies virus CVS Phosphoprotein Isoform 3 (CVS P3)

Phosphoprotein (Isoform P3; C297S) experimental SAS data
Rabies virus CVS Phosphoprotein Isoform 3 (CVS P3) Rg histogram
Sample: Phosphoprotein (Isoform P3; C297S) dimer, 56 kDa Rabies virus (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Mar 7
Conformational dynamics, RNA binding, and phase separation regulate the multifunctionality of rabies virus P protein. Nat Commun 16(1):9491 (2025)
...Sethi A, David CT, Harrison AR, Bird LE, Rozario AM, Uthishtran S, Ardipradja K, Zhao T, Oksayan S, Jans DA, Ang CS, Lu ZH, Yan F, Williamson NA, Arumugam S, Sundaramoorthy V, Bell TDM, Gooley PR, Mos...
RgGuinier 4.2 nm
Dmax 17.0 nm
VolumePorod 141 nm3

SASDRZ4 – K214/R260 mutant of Rabies virus CVS Phosphoprotein Isoform 3 (KRm CVS P3)

Phosphoprotein (Isoform P3; K214A, R260A, D289N, C297S) experimental SAS data
EOM/RANCH model
Sample: Phosphoprotein (Isoform P3; K214A, R260A, D289N, C297S) dimer, 55 kDa Rabies virus (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Mar 7
Conformational dynamics, RNA binding, and phase separation regulate the multifunctionality of rabies virus P protein. Nat Commun 16(1):9491 (2025)
...Sethi A, David CT, Harrison AR, Bird LE, Rozario AM, Uthishtran S, Ardipradja K, Zhao T, Oksayan S, Jans DA, Ang CS, Lu ZH, Yan F, Williamson NA, Arumugam S, Sundaramoorthy V, Bell TDM, Gooley PR, Mos...
RgGuinier 4.6 nm
Dmax 22.5 nm
VolumePorod 180 nm3

SASDRY4 – D289N mutant of Rabies virus CVS Phosphoprotein Isoform 3 (CVS P3)

Phosphoprotein (Isoform P3; D289N, C297S) experimental SAS data
D289N mutant of Rabies virus CVS Phosphoprotein Isoform 3 (CVS P3) Rg histogram
Sample: Phosphoprotein (Isoform P3; D289N, C297S) dimer, 56 kDa Rabies virus (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Mar 7
Conformational dynamics, RNA binding, and phase separation regulate the multifunctionality of rabies virus P protein. Nat Commun 16(1):9491 (2025)
...Sethi A, David CT, Harrison AR, Bird LE, Rozario AM, Uthishtran S, Ardipradja K, Zhao T, Oksayan S, Jans DA, Ang CS, Lu ZH, Yan F, Williamson NA, Arumugam S, Sundaramoorthy V, Bell TDM, Gooley PR, Mos...
RgGuinier 3.9 nm
Dmax 17.0 nm
VolumePorod 128 nm3