Small Angle Scattering Biological Data Bank SASBDB


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11 hits found for Sethi

SASDV64 – Serine hydrolase FphH with short chain esterase activity (Apo FphH)

Alpha/beta fold hydrolase experimental SAS data

Sample:	Alpha/beta fold hydrolase monomer, 28 kDa Staphylococcus aureus protein
Buffer:	100 mM NaCl, 10 mM HEPES, pH: 7.6
Experiment:	SAXS data collected at BioSAXS, Australian Synchrotron on 2024 Apr 11

Similar but Distinct—Biochemical Characterization of the Staphylococcus aureus Serine Hydrolases FphH and FphI Proteins: Structure, Function, and Bioinformatics (2024)
...Sethi A, Jelinek R, Kass I

R_g^Guinier	2.1	nm
D_max	6.5	nm
Volume^Porod	41	nm³

SASDXD4 – Full-length human SFPQ dimer (protiated and deuterated)

Splicing factor, proline- and glutamine-rich experimental SAS data

Splicing factor, proline- and glutamine-rich Kratky plot

Sample:	Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer:	150 mM KCl, 1.5% glycerol, 20 mM HEPES, 1 mM DTT (in 100% H2O), pH: 7.4
Experiment:	SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2023 Jan 23

Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
...Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C

R_g^Guinier	8.7	nm
D_max	30.7	nm
Volume^Porod	241000	nm³

SASDQG4 – Rabies virus Nishigahara strain Phosphoprotein Isoform 3 (P3)

Isoform P3 of Phosphoprotein experimental SAS data

Rabies virus Nishigahara strain Phosphoprotein Isoform 3 (P3) Rg histogram

Sample:	Isoform P3 of Phosphoprotein dimer, 55 kDa Rabies virus (strain … protein
Buffer:	25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 26

Structural insights into the multifunctionality of rabies virus P3 protein. Proc Natl Acad Sci U S A 120(14):e2217066120 (2023)
Sethi A, Rawlinson SM, Dubey A, Ang CS, Choi YH, Yan F, Okada K, Rozario AM, Brice AM, Ito N, Williamson NA, Hatters DM, Bell TDM, Arthanari H, Moseley GW, Gooley PR

R_g^Guinier	3.7	nm
D_max	16.5	nm
Volume^Porod	108	nm³

SASDQH4 – Attenuated Nishigahara Phosphoprotein Isoform 3 (Ni-CE P3)

Attenuated derivative P3 of Phosphoprotein experimental SAS data

Attenuated Nishigahara Phosphoprotein Isoform 3 (Ni-CE P3) Rg histogram

Sample:	Attenuated derivative P3 of Phosphoprotein dimer, 55 kDa protein
Buffer:	25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 26

R_g^Guinier	4.0	nm
D_max	17.5	nm
Volume^Porod	127	nm³

SASDQJ4 – N226H Nishigahara Phosphoprotein Isoform 3 (N226H_P3)

Isoform P3 of Phosphoprotein Nish P3 N226H experimental SAS data

N226H Nishigahara Phosphoprotein Isoform 3 (N226H_P3) Rg histogram

Sample:	Isoform P3 of Phosphoprotein Nish P3 N226H dimer, 55 kDa protein
Buffer:	25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 26

R_g^Guinier	4.3	nm
D_max	19.0	nm
Volume^Porod	150	nm³

SASDV57 – Full-length SFPQ in high salt buffer

Sample:	Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer:	500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Oct 22

R_g^Guinier	7.9	nm
D_max	42.5	nm
Volume^Porod	485	nm³

SASDV67 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707)

Splicing Factor Proline/Glutamine rich (1-598) experimental SAS data

Splicing Factor Proline/Glutamine rich (1-598) Kratky plot

Sample:	Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein
Buffer:	500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 13

R_g^Guinier	7.6	nm
D_max	34.3	nm
Volume^Porod	400	nm³

SASDV77 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707) in low salt buffer

Sample:	Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein
Buffer:	150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 13

R_g^Guinier	6.1	nm
D_max	28.0	nm
Volume^Porod	315	nm³

SASDLG7 – LDLa linker region of human Relaxin receptor 1 (RXFP1, 23-94)

Relaxin receptor 1 experimental SAS data

LDLa linker region of human Relaxin receptor 1 (RXFP1, 23-94) Rg histogram

Sample:	Relaxin receptor 1 monomer, 8 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.1%NaN3, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 6

Structural Insights into the Unique Modes of Relaxin-Binding and Tethered-Agonist Mediated Activation of RXFP1 and RXFP2. J Mol Biol 433(21):167217 (2021)
Sethi A, Bruell S, Ryan T, Yan F, Tanipour MH, Mok YF, Draper-Joyce C, Khandokar Y, Metcalfe RD, Griffin MDW, Scott DJ, Hossain MA, Petrie EJ, Bathgate RAD, Gooley PR

R_g^Guinier	2.1	nm
D_max	10.0	nm
Volume^Porod	19	nm³

SASDLH7 – LDLa linker region of human Relaxin receptor 2 (RXFP2, 38-105)

Relaxin receptor 2 experimental SAS data

LDLa linker region of human Relaxin receptor 2 (RXFP2, 38-105) Rg histogram

Sample:	Relaxin receptor 2 monomer, 7 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.1%NaN3, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 6

R_g^Guinier	1.7	nm
D_max	7.1	nm
Volume^Porod	12	nm³

SASDV59 – Human Splicing factor, proline- and glutamine-rich protein 5% SFPQ and 95% dSFPQ at a D2O matchpoint of 95%

Sample:	Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer:	150 mM KCl, 0.075% glycerol, 20 mM HEPES, 0.5 mM DTT,, pH: 7.4
Experiment:	SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2023 Jan 20

R_g^Guinier	6.1	nm
D_max	22.0	nm
Volume^Porod	181	nm³

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