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4 hits found for Tsutakawa

SASDT55 – Non-structural protein 1 (NS1B, C-terminal) with 5' PPP hairpin RNA

Non-structural protein 15’ppp ds10 HP RNA experimental SAS data
HADDOCK model
Sample: Non-structural protein 1 monomer, 16 kDa Influenza B virus protein
5’ppp ds10 HP RNA monomer, 8 kDa RNA
Buffer: 40 mM ammonium acetate, pH 5.5, 225 mM NaCl, 5 mM CaCl2, 0.02% NaN3, 50 mM arginine, pH: 5.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Aug 6
The NS1 protein of influenza B virus binds 5’-triphosphorylated dsRNA to suppress RIG-I activation and the host antiviral response (2024)
...Tsutakawa S, Zhao C, Ma L, Shurina B, Hura G, John R, Vorobiev S, Swapna G, Solotchi M, Tainer J, Krug R, Patel S, Montelione G
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 28 nm3

SASDT65 – Non-structural protein 1 (NS1B, C-terminal) plus double stranded RNA

Non-structural protein 1RNA top strandRNA bottom strand experimental SAS data
HADDOCK model
Sample: Non-structural protein 1 monomer, 16 kDa Influenza B virus protein
RNA top strand monomer, 5 kDa RNA
RNA bottom strand monomer, 5 kDa RNA
Buffer: 40 mM ammonium acetate, pH 5.5, 450 mM NaCl, 5 mM CaCl2, 0.02% NaN3, 50 mM arginine, pH: 5.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 18
The NS1 protein of influenza B virus binds 5’-triphosphorylated dsRNA to suppress RIG-I activation and the host antiviral response (2024)
...Tsutakawa S, Zhao C, Ma L, Shurina B, Hura G, John R, Vorobiev S, Swapna G, Solotchi M, Tainer J, Krug R, Patel S, Montelione G
RgGuinier 2.8 nm
Dmax 10.3 nm
VolumePorod 23 nm3

SASDJ95 – E3 ubiquitin-protein ligase BRE1 complexed with E2 ubiquitin conjugating enzyme RAD6

E3 ubiquitin-protein ligase BRE1Ubiquitin-conjugating enzyme E2 2 experimental SAS data
SREFLEX model
Sample: E3 ubiquitin-protein ligase BRE1 dimer, 50 kDa Saccharomyces cerevisiae (strain … protein
Ubiquitin-conjugating enzyme E2 2 monomer, 20 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Feb 11
Structural basis for the role of C-terminus acidic tail of Saccharomyces cerevisiae ubiquitin-conjugating enzyme (Rad6) in E3 ligase (Bre1) mediated recognition of histones International Journal of Biological Macromolecules :127717 (2023)
...Tsutakawa S, Kamthan M, Kumar P
RgGuinier 4.1 nm
Dmax 10.4 nm
VolumePorod 105 nm3

SASDJA5 – E2 ubiquitin conjugating enzyme RAD6

Ubiquitin-conjugating enzyme E2 2 experimental SAS data
SREFLEX model
Sample: Ubiquitin-conjugating enzyme E2 2 monomer, 20 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Feb 11
Structural basis for the role of C-terminus acidic tail of Saccharomyces cerevisiae ubiquitin-conjugating enzyme (Rad6) in E3 ligase (Bre1) mediated recognition of histones International Journal of Biological Macromolecules :127717 (2023)
...Tsutakawa S, Kamthan M, Kumar P
RgGuinier 2.3 nm
Dmax 6.6 nm
VolumePorod 31 nm3