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10 hits found for Witte

SASDK74 – Streptococcus agalactiae transcription factor BusR - RCK_C domain

Transcriptional repressor BusR RCK_C domain experimental SAS data
OTHER model
Sample: Transcriptional repressor BusR RCK_C domain dimer, 22 kDa Streptococcus agalactiae serotype … protein
Buffer: 100 mM NaCl, 30mM Hepes, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2
BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture. Nucleic Acids Res (2021)
...Witte G
RgGuinier 1.9 nm
Dmax 6.4 nm
VolumePorod 44 nm3

SASDK84 – Streptococcus agalactiae transcription factor BusR

Transcriptional repressor BusR experimental SAS data
Transcriptional repressor BusR Kratky plot
Sample: Transcriptional repressor BusR tetramer, 95 kDa Streptococcus agalactiae protein
Buffer: 20mM HEPES, pH6.5, 100mM NaCl, 3% glycerol (v/v), pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2
BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture. Nucleic Acids Res (2021)
...Witte G
RgGuinier 4.4 nm
Dmax 13.9 nm
VolumePorod 168 nm3

SASDK94 – Streptococcus agalactiae transcription factor BusR dsDNA complex

Transcriptional repressor BusRBusR Recognition sequence experimental SAS data
OTHER model
Sample: Transcriptional repressor BusR tetramer, 95 kDa Streptococcus agalactiae protein
BusR Recognition sequence monomer, 28 kDa synthetic construct DNA
Buffer: 20mM HEPES, pH6.5, 100mM NaCl, 3% glycerol (v/v), pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2
BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture. Nucleic Acids Res (2021)
...Witte G
RgGuinier 4.3 nm
Dmax 14.2 nm
VolumePorod 210 nm3

SASDH25 – Actinobacterial phosphodiesterase targeting cyclic di-AMP (AtaC)

Alkaline phosphodiesterase I or Nucleotide pyrophosphatase experimental SAS data
Alkaline phosphodiesterase I or Nucleotide pyrophosphatase Kratky plot
Sample: Alkaline phosphodiesterase I or Nucleotide pyrophosphatase monomer, 46 kDa Streptomyces venezuelae protein
Buffer: 20 mM HEPES, 200mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 17
c-di-AMP hydrolysis by a novel type of phosphodiesterase promotes differentiation of multicellular bacteria (2019)
...Witte G, Tschowri N
RgGuinier 2.4 nm
Dmax 7.8 nm
VolumePorod 74 nm3

SASDH35 – Soluble cyclic di-AMP binding protein CpeA (vnz_28055), regulator of CpeB

Cyclic di-AMP binding protein (Putative regulatory, ligand-binding protein) experimental SAS data
Cyclic di-AMP binding protein (Putative regulatory, ligand-binding protein) Kratky plot
Sample: Cyclic di-AMP binding protein (Putative regulatory, ligand-binding protein) dimer, 38 kDa Streptomyces venezuelae protein
Buffer: 200 mM NaCl, 30 mM NaPi, 5% (v/v) glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 4
c-di-AMP hydrolysis by a novel type of phosphodiesterase promotes differentiation of multicellular bacteria (2019)
...Witte G, Tschowri N
RgGuinier 2.8 nm
Dmax 9.2 nm
VolumePorod 69 nm3

SASDXD5 – S.aureus PstA in a ligand-free state (cyclic-di-AMP receptor)

Cyclic-di-AMP receptor experimental SAS data
Cyclic-di-AMP receptor Kratky plot
Sample: Cyclic-di-AMP receptor trimer, 42 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 11
Activation of a Secondary-Messenger Receptor via Allosteric Modulation of a Dynamic Conformational Ensemble. Angew Chem Int Ed Engl :e202509394 (2025)
...Witte G, Linser R
RgGuinier 2.7 nm
Dmax 11.0 nm
VolumePorod 91 nm3

SASDXE5 – S.aureus PstA with c-di-AMP (cyclic-di-AMP receptor)

Cyclic-di-AMP receptor experimental SAS data
Cyclic-di-AMP receptor Kratky plot
Sample: Cyclic-di-AMP receptor trimer, 42 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 11
Activation of a Secondary-Messenger Receptor via Allosteric Modulation of a Dynamic Conformational Ensemble. Angew Chem Int Ed Engl :e202509394 (2025)
...Witte G, Linser R
RgGuinier 2.6 nm
Dmax 11.0 nm
VolumePorod 86 nm3

SASDCB7 – Streptococcus pneumoniae phosphodiesterase Pde2

DHH subfamily 1 protein experimental SAS data
DHH subfamily 1 protein Kratky plot
Sample: DHH subfamily 1 protein dimer, 70 kDa Streptococcus pneumoniae serotype … protein
Buffer: 20mM Tris, 200 mM NaCl, 5%(v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 23
Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima. Structure 25(12):1887-1897.e4 (2017)
...Witte G
RgGuinier 2.7 nm
Dmax 7.7 nm
VolumePorod 87 nm3

SASDCC7 – Thermotoga martitima phosphodiesterase TM1595 D80N D154N (inactive mutant)

T.maritima PDE experimental SAS data
T.maritima PDE Kratky plot
Sample: T.maritima PDE dimer, 76 kDa Thermotoga maritima protein
Buffer: 25mM Tris 500mM NaCl 3% (v/v) glycerol 2mM MgCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 17
Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima. Structure 25(12):1887-1897.e4 (2017)
...Witte G
RgGuinier 2.8 nm
Dmax 7.9 nm
VolumePorod 115 nm3

SASDCD7 – Thermotoga maritima phosphodiesterase TM1595 (wildtype, TmPde)

Thermotoga maritima phosphodiesterase (wildtype, TmPDE, TM1595) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Thermotoga maritima phosphodiesterase (wildtype, TmPDE, TM1595) dimer, 76 kDa Thermotoga maritima protein
Buffer: 20mM Tris, 200 mM NaCl, 5%(v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 23
Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima. Structure 25(12):1887-1897.e4 (2017)
...Witte G
RgGuinier 2.7 nm
Dmax 7.8 nm
VolumePorod 106 nm3