Small Angle Scattering Biological Data Bank SASBDB


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16 hits found for Gag polyprotein

SASDNH3 – HIV-1 reverse transcriptase L289K mutation at 1 mg/mL

Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) experimental SAS data

Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) Kratky plot

Sample:	Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer:	50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20

Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R

R_g^Guinier	3.2	nm
D_max	10.7	nm
Volume^Porod	91	nm³

SASD2H3 – ...Gag Polyprotein at 8mg/ml

Sample:	Gag polyprotein trimer, 150 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris, 500 mM NaCl, 1 mM phenylmethylsulfonyl fluoride, 10 mM beta-mercaproethanol, 1 µM ZnCl2, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 24

...Gag polyprotein in solution. J Struct Biol 218(4):108372 (2026)
Shtykova EV, Petoukhov MV, Dadinova LA, Denieva ZG, Batishchev OV

R_g^Guinier	4.8	nm
D_max	18.0	nm
Volume^Porod	202	nm³

SASDNJ3 – HIV-1 reverse transcriptase L289K mutation at 2 mg/mL

Sample:	Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer:	50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20

Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R

R_g^Guinier	3.2	nm
D_max	10.8	nm
Volume^Porod	91	nm³

SASDNK3 – HIV-1 reverse transcriptase L289K mutation at 4.3 mg/mL

Sample:	Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer:	50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20

Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R

R_g^Guinier	3.2	nm
D_max	10.7	nm
Volume^Porod	93	nm³

SASDNL3 – HIV-1 reverse transcriptase L289K mutation (extrapolated to zero concentration)

Sample:	Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer:	50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 20

Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R

R_g^Guinier	3.2	nm
D_max	11.1	nm
Volume^Porod	90	nm³

SASDKR3 – Deuterated calmodulin bound to the HIV-1 MA protein

Calmodulin-1calcium ionsGag-Pol polyprotein experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Xenopus laevis protein calcium ions tetramer, 0 kDa Gag-Pol polyprotein monomer, 15 kDa Human immunodeficiency virus … protein
Buffer:	50 mM MOPS, 5 mM CaCl2, 2 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at Bruker Nanostar, Australian Nuclear Science and Technology Organisation on 2010 Jun 23

Calmodulin binds a highly extended HIV-1 MA protein that refolds upon its release. Biophys J 103(3):541-549 (2012)
Taylor JE, Chow JYH, Jeffries CM, Kwan AH, Duff AP, Hamilton WA, Trewhella J

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	55	nm³

SASDSZ4 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) unliganded form

Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66)Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) experimental SAS data

Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) Kratky plot

Sample:	Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K

R_g^Guinier	3.3	nm
D_max	9.9	nm
Volume^Porod	153	nm³

SASDS25 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) bound with Nevirapine

Sample:	Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	3.5	nm
D_max	10.9	nm
Volume^Porod	161	nm³

SASDS35 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) unliganded form

Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) experimental SAS data

Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) Kratky plot

Sample:	Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	3.1	nm
D_max	10.7	nm
Volume^Porod	98	nm³

SASDS45 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) bound with Efavirenz

Sample:	Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	3.7	nm
D_max	11.3	nm
Volume^Porod	178	nm³

SASDS55 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) bound with Nevirapine

Sample:	Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	3.5	nm
D_max	11.3	nm
Volume^Porod	134	nm³

SASDS65 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) bound with Efavirenz

Sample:	Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	4.6	nm
D_max	15.0	nm
Volume^Porod	270	nm³

SASDS75 – HIV-1 Reverse Transcriptase monomer (p51 subunit with W401A mutation) unliganded form

Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) experimental SAS data

Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) Kratky plot

Sample:	Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	2.8	nm
D_max	9.8	nm
Volume^Porod	84	nm³

SASDS85 – HIV-1 Reverse Transcriptase monomer (p51 subunit with W401A mutation) bound with Nevirapine

Sample:	Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	3.1	nm
D_max	11.1	nm
Volume^Porod	103	nm³

SASDS95 – HIV-1 Reverse Transcriptase monomer (p51 subunit with W401A mutation) bound with Efavirenz

Sample:	Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	4.2	nm
D_max	16.7	nm
Volume^Porod	226	nm³

SASD2E6 – ...Gag Polyprotein at 2 mg/ml

Human immunodeficiency virus type 1 (HIV-1) Gag Polyprotein at 2 mg/ml Rg histogram

Sample:	Gag polyprotein monomer, 50 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris, 500 mM NaCl, 1 mM phenylmethylsulfonyl fluoride, 10 mM beta-mercaproethanol, 1 µM ZnCl2, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 24

...Gag polyprotein in solution. J Struct Biol 218(4):108372 (2026)
Shtykova EV, Petoukhov MV, Dadinova LA, Denieva ZG, Batishchev OV

R_g^Guinier	4.1	nm
D_max	16.0	nm
Volume^Porod	64	nm³

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